Biochemistry Techniques Quiz
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Questions and Answers

What is a contrast-enhancing optical technique used to produce high-contrast images of transparent specimens such as living cells and microorganisms?

  • Fluorescence
  • Confocal
  • Differential Interference Contrast (DIC)
  • Phase Contrast (correct)
  • Which microscopy technique utilizes the difference in optical path lengths in transparent specimens to render contrast?

  • Electrophoresis
  • Phase Contrast
  • Differential Interference Contrast (DIC) (correct)
  • ELISA
  • Which of the following is not a type of microscopy mentioned in the text?

  • Cryo-electron
  • Multiphoton
  • Light Sheet
  • PCR (correct)
  • Which microscopy technique involves the use of laser scanning to create detailed images of biological specimens?

    <p>Confocal</p> Signup and view all the answers

    What is an excellent mechanism for rendering contrast in transparent specimens based on the difference in optical path lengths?

    <p>Differential Interference Contrast (DIC)</p> Signup and view all the answers

    What is the purpose of confocal laser scanning microscopy (CLSM) in the life sciences?

    <p>To capture multiple two-dimensional images at different depths in a sample</p> Signup and view all the answers

    In phase contrast microscopy, what is the distinctive feature surrounding the cells?

    <p>Halo artifacts</p> Signup and view all the answers

    What does Polymerase Chain Reaction (PCR) aim to achieve?

    <p>Replicating DNA to generate thousands to millions of copies of a particular DNA sequence</p> Signup and view all the answers

    What technique is commonly used for separating a mixture of chemical substances into its individual components?

    <p>Chromatography</p> Signup and view all the answers

    What variation in temperature is used in Polymerase Chain Reaction (PCR) for controlling the replication process?

    <p>~95ºC for 1 min</p> Signup and view all the answers

    What is the distinctive feature of phase contrast microscopy?

    <p>Enhances contrast in transparent specimens using a special mechanism</p> Signup and view all the answers

    Which microscopy technique involves beams passing through different parts of the specimen with different lengths, resulting in regions appearing brighter or darker?

    <p>Confocal microscopy</p> Signup and view all the answers

    What is an application of phase contrast microscopy?

    <p>Rendering contrast in transparent specimens based on differences in optical path lengths</p> Signup and view all the answers

    In which technique do regions of the specimen appear brighter or darker based on differences in optical path lengths?

    <p>Multiphoton microscopy</p> Signup and view all the answers

    Which microscopy technique utilizes a special mechanism to enhance contrast in transparent specimens such as living cells and microorganisms?

    <p>Fluorescence microscopy</p> Signup and view all the answers

    What is the primary purpose of Differential Interference Contrast (DIC) microscopy?

    <p>Renders contrast in transparent specimens based on differences in optical path lengths</p> Signup and view all the answers

    What is the purpose of high performance liquid chromatography (HPLC)?

    <p>Analyzing individual components of a chemical mixture</p> Signup and view all the answers

    What is the distinctive feature surrounding cells in phase contrast microscopy?

    <p>Halo artifacts</p> Signup and view all the answers

    What does confocal laser scanning microscopy (CLSM) primarily aim to achieve?

    <p>Imaging living specimens and capturing 3D-data</p> Signup and view all the answers

    What is the main objective of polymerase chain reaction (PCR)?

    <p>Amplifying specific segments of DNA to generate millions of copies</p> Signup and view all the answers

    In DIC microscopy, what is imaged more clearly than with phase contrast?

    <p>Periphery of cellular membranes</p> Signup and view all the answers

    Which technique uses variations in temperature to control the replication process?

    <p>Polymerase Chain Reaction (PCR)</p> Signup and view all the answers

    What is the distinctive feature of pseudo three-dimensional relief shading in microscopy?

    <p>Shadow-cast appearance</p> Signup and view all the answers

    What does column chromatography primarily aim to achieve?

    <p>Separating a mixture of chemical substances into individual components</p> Signup and view all the answers

    What is the main function of gas chromatography (GC)?

    <p>Separating a mixture of chemical substances into individual components</p> Signup and view all the answers

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