Biochemistry Techniques Quiz
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Questions and Answers

What is a contrast-enhancing optical technique used to produce high-contrast images of transparent specimens such as living cells and microorganisms?

  • Fluorescence
  • Confocal
  • Differential Interference Contrast (DIC)
  • Phase Contrast (correct)

Which microscopy technique utilizes the difference in optical path lengths in transparent specimens to render contrast?

  • Electrophoresis
  • Phase Contrast
  • Differential Interference Contrast (DIC) (correct)
  • ELISA

Which of the following is not a type of microscopy mentioned in the text?

  • Cryo-electron
  • Multiphoton
  • Light Sheet
  • PCR (correct)

Which microscopy technique involves the use of laser scanning to create detailed images of biological specimens?

<p>Confocal (D)</p> Signup and view all the answers

What is an excellent mechanism for rendering contrast in transparent specimens based on the difference in optical path lengths?

<p>Differential Interference Contrast (DIC) (A)</p> Signup and view all the answers

What is the purpose of confocal laser scanning microscopy (CLSM) in the life sciences?

<p>To capture multiple two-dimensional images at different depths in a sample (A)</p> Signup and view all the answers

In phase contrast microscopy, what is the distinctive feature surrounding the cells?

<p>Halo artifacts (D)</p> Signup and view all the answers

What does Polymerase Chain Reaction (PCR) aim to achieve?

<p>Replicating DNA to generate thousands to millions of copies of a particular DNA sequence (D)</p> Signup and view all the answers

What technique is commonly used for separating a mixture of chemical substances into its individual components?

<p>Chromatography (D)</p> Signup and view all the answers

What variation in temperature is used in Polymerase Chain Reaction (PCR) for controlling the replication process?

<p>~95ºC for 1 min (B)</p> Signup and view all the answers

What is the distinctive feature of phase contrast microscopy?

<p>Enhances contrast in transparent specimens using a special mechanism (D)</p> Signup and view all the answers

Which microscopy technique involves beams passing through different parts of the specimen with different lengths, resulting in regions appearing brighter or darker?

<p>Confocal microscopy (A)</p> Signup and view all the answers

What is an application of phase contrast microscopy?

<p>Rendering contrast in transparent specimens based on differences in optical path lengths (C)</p> Signup and view all the answers

In which technique do regions of the specimen appear brighter or darker based on differences in optical path lengths?

<p>Multiphoton microscopy (C)</p> Signup and view all the answers

Which microscopy technique utilizes a special mechanism to enhance contrast in transparent specimens such as living cells and microorganisms?

<p>Fluorescence microscopy (C)</p> Signup and view all the answers

What is the primary purpose of Differential Interference Contrast (DIC) microscopy?

<p>Renders contrast in transparent specimens based on differences in optical path lengths (B)</p> Signup and view all the answers

What is the purpose of high performance liquid chromatography (HPLC)?

<p>Analyzing individual components of a chemical mixture (A)</p> Signup and view all the answers

What is the distinctive feature surrounding cells in phase contrast microscopy?

<p>Halo artifacts (D)</p> Signup and view all the answers

What does confocal laser scanning microscopy (CLSM) primarily aim to achieve?

<p>Imaging living specimens and capturing 3D-data (A)</p> Signup and view all the answers

What is the main objective of polymerase chain reaction (PCR)?

<p>Amplifying specific segments of DNA to generate millions of copies (B)</p> Signup and view all the answers

In DIC microscopy, what is imaged more clearly than with phase contrast?

<p>Periphery of cellular membranes (A)</p> Signup and view all the answers

Which technique uses variations in temperature to control the replication process?

<p>Polymerase Chain Reaction (PCR) (D)</p> Signup and view all the answers

What is the distinctive feature of pseudo three-dimensional relief shading in microscopy?

<p>Shadow-cast appearance (C)</p> Signup and view all the answers

What does column chromatography primarily aim to achieve?

<p>Separating a mixture of chemical substances into individual components (C)</p> Signup and view all the answers

What is the main function of gas chromatography (GC)?

<p>Separating a mixture of chemical substances into individual components (C)</p> Signup and view all the answers

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