Biochemistry Lab Safety Guidelines

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Questions and Answers

Which of the following is NOT a special precaution for working with heat or fire?

  • Inflammable liquid bottles should not be dispensed near a naked flame.
  • Never handle hot materials without wearing gloves. (correct)
  • Never leave a lighted Bunsen burner unattended.
  • Use test tube holders to handle hot laboratory equipment.
  • Use only Pyrex glassware's for heating.

What are the main types of hazards that laboratory safety symbols are designed to warn people of?

  • Physical and chemical hazards
  • Biological and chemical hazards
  • All potential hazards (correct)
  • Electrical hazards only
  • Chemical hazards only

It is acceptable to leave a hot object unattended after removing it from a heat source.

False (B)

When diluting acid, what is the correct procedure?

<p>Add acid to water. (A)</p> Signup and view all the answers

Always smell substances in the laboratory without specific instructions.

<p>False (B)</p> Signup and view all the answers

A good way to prevent accidents is to wear open shoes, such as sandals, when working in a laboratory.

<p>False (B)</p> Signup and view all the answers

When working with chemicals, it is crucial to ensure all containers are ______.

<p>properly labeled</p> Signup and view all the answers

What is the primary purpose of a lab coat?

<p>To protect the wearer's clothes from splashes, chemicals, and other contaminants.</p> Signup and view all the answers

It is acceptable to leave unused materials on the laboratory floor.

<p>False (B)</p> Signup and view all the answers

When working in a laboratory, it is okay to run in case of an emergency if you need to reach a specific location quickly.

<p>False (B)</p> Signup and view all the answers

What should be done if you find damaged laboratory apparatus?

<p>Remove it immediately.</p> Signup and view all the answers

Why is it important to wash your hands after working with chemicals?

<p>To prevent accidental ingestion or transfer of chemicals to other parts of the body or to other objects.</p> Signup and view all the answers

What steps should be taken if a chemical is accidentally swallowed?

<p>Give the person water immediately. If the chemical is an acid, give them milk to drink. If the chemical is an alkali, give them diluted acetic acid.</p> Signup and view all the answers

Which of the following symbols represents a corrosive substance?

<p>Hand with corrosive damage (C)</p> Signup and view all the answers

What are two reasons why it's important to keep exits and passageways clear in the laboratory?

<p>Clear exits and passageways allow for safe and quick evacuation in case of an emergency, and they prevent accidents or falls due to obstructed paths.</p> Signup and view all the answers

It is acceptable to use broken or chipped glassware in the laboratory.

<p>False (B)</p> Signup and view all the answers

What should be done to clean broken glass safely in the laboratory?

<p>Pick up broken glass with a broom and dustpan, then dispose of it in a designated sharps container.</p> Signup and view all the answers

What is the purpose of laboratory safety symbols?

<p>Laboratory safety symbols are used to quickly and easily communicate potential hazards to people working in the lab.</p> Signup and view all the answers

Chemicals labeled as toxic can cause death if they penetrate the skin, are inhaled, or are swallowed.

<p>True (A)</p> Signup and view all the answers

Flammable substances are not hazardous if they are kept away from open flames and electricity.

<p>False (B)</p> Signup and view all the answers

Where should a hot object be placed after it has been removed from a heat source?

<p>In a designated area where it can cool and be shielded from contact. (C)</p> Signup and view all the answers

It is safe to leave flammable liquid bottles open in a laboratory even if they are not near heat.

<p>False (B)</p> Signup and view all the answers

Describe the appropriate procedure for cleaning up a chemical spill on the floor.

<p>First, wear appropriate protective gear, such as gloves and a lab coat. Then, use an absorbent material to soak up the spill and place it in a designated chemical waste container.</p> Signup and view all the answers

Why is it important to use only Pyrex glassware for heating?

<p>Pyrex glassware is designed to resist heat and thermal shock, making it suitable for use in heating processes. Regular glassware can crack or shatter when exposed to sudden temperature changes.</p> Signup and view all the answers

It is acceptable to work with live animals in a laboratory without any specific instructions.

<p>False (B)</p> Signup and view all the answers

Laboratory safety symbols are designed to warn people only about the potential dangers of chemical substances.

<p>False (B)</p> Signup and view all the answers

The symbols used to communicate laboratory safety are considered universal and are understood by people from different cultures.

<p>True (A)</p> Signup and view all the answers

It is acceptable to unplug an appliance without turning off the power switch.

<p>False (B)</p> Signup and view all the answers

What are some examples of common laboratory equipment that might be dangerous to handle if it is hot?

<p>Examples include heating elements, Bunsen burners, test tubes, beakers, flasks, glassware, and metal objects.</p> Signup and view all the answers

It is better to wear gloves than to wash your hands when working with chemicals.

<p>False (B)</p> Signup and view all the answers

It is okay to work in a laboratory with wet hands.

<p>False (B)</p> Signup and view all the answers

What are the risks of working with live cultures in a laboratory?

<p>Working with live cultures poses a risk of infection if not handled properly. Accidental exposure can lead to illness.</p> Signup and view all the answers

What is the purpose of detergents in a laboratory?

<p>Detergents are used to sterilize surfaces, equipment, and glassware to kill microorganisms and reduce the risk of contamination.</p> Signup and view all the answers

Wearing a lab coat in the laboratory is only necessary when working with chemicals.

<p>False (B)</p> Signup and view all the answers

Flashcards

Laboratory Safety

Practices to prevent accidents and injuries in labs.

Hazardous Materials

Materials that can cause health issues or damage.

Lab Coat Requirements

Wear a closed, clean lab coat knee-length at all times.

No Eating or Drinking

Avoid eating, drinking, smoking in the lab.

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Handling Chemicals

Follow specific steps when working with chemicals.

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Heat Precautions

Never leave hot items unattended, point away from people.

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Glassware Safety

Never use broken or chipped glassware in a lab.

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Electrical Safety

Keep electrical equipment dry and free of flammables.

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Emergency First Aid

Basic procedures for managing injuries in the lab.

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Chemical Handling Tips

Never taste or smell substances in the lab.

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Personal Hygiene in Lab

Always wash hands after working with materials.

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Petri Dish Safety

Open only under supervision to avoid contamination.

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Toxic Chemicals

Substances that can cause death through various exposures.

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Flammable Materials

Substances that can ignite easily and burn.

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Corrosive Chemicals

Substances that destroy soft tissues upon contact.

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Specimen Care

Handle live or preserved specimens with care.

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Bunsen Burner Safety

Never leave a lit burner unattended; keep away from flammable items.

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Lab Equipment Familiarity

Know the names and functions of equipment before use.

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Chemical Disposal

Return unused materials to their proper locations safely.

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Using Test Tube Holders

Always use holders for handling hot test tubes.

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Cleaning Glassware

Ensure all glassware is clean before use to avoid contamination.

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Use of Detergents

Use detergents to sterilize after working with specimens.

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Hoses and Equipment Checks

Ensure Bunsen burner hoses fit tightly to prevent leaks.

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Proper Use of Electrical Devices

Always turn off power switches before plugging appliances.

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Safety in Lab

Practices to ensure safety while working in a lab setting.

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Hazard Awareness

Understanding the dangers of materials in the lab before use.

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Lab Equipment Handling

Correct methods to handle laboratory materials to prevent accidents.

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Children in Labs

Children are strictly forbidden in the laboratory environment.

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Restricted Actions

Eating, drinking, or applying cosmetics is prohibited in labs.

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Lab Coat Policy

Mandatory wear of a closed, clean lab coat at all times.

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Footwear Guidelines

Open shoes or sandals are not allowed in the laboratory.

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Movement in Lab

Walking, not running, is required in the laboratory.

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Clear Exits

Exits and passageways in the lab must be kept unobstructed.

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Material Use Policy

Work only with approved materials assigned during training.

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Discarding Damaged Equipment

Damaged apparatus must be removed immediately to prevent injury.

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Reagent Storage

Reagent bottles should never be left unattended on the floor.

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Apparatus Cleanup

Washing used apparatus is essential before leaving the lab.

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Hand Hygiene

Washing hands after laboratory work to maintain cleanliness.

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Hazardous Material Definition

Materials that can harm health, property, or the environment.

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Toxic Chemical Symbol

Symbol indicating materials that can cause death or injury.

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Usage of Inflammable Materials

Inflammable substances must be stored away from flames and heat.

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Using Test Tube Holders Correctly

Always use holders to manage hot test tubes safely.

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Pyrex Glassware

Always use Pyrex for heating to prevent breakage.

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Dilution of Acid

Add acid to water, not the other way around to prevent reactions.

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Electrical Safety Checks

Ensure surroundings of electrical equipment are dry and clean.

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Cleanliness with Glassware

Ensure glassware is clean and unbroken before use to avoid contamination.

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Handling Live Specimens

Treat live animals gently and always wash hands after use.

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Dissection Practices

Specimens for dissection should be supported and secured during cutting.

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Detergents for Sterilization

Detergents should be used to clean benches and glassware after use.

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Avoiding Broken Glass

Never pick up broken glass with bare hands; use proper tools instead.

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Chemical Warning Symbols

Visual symbols indicating potential hazards in the lab environment.

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First Aid for Burns

Immediately wash burns with water and seek proper treatment.

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Responding to Poisoning

Rinse mouth with water if toxic chemicals are swallowed.

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General Lab Safety Knowledge

Understanding and following lab safety protocols is crucial.

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Biohazard Awareness

Recognizing and managing risks associated with biological specimens.

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Cold and Warming Equipment Safety

Always cool down hot materials before transporting to avoid burns.

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Study Notes

UMST Preparatory College Biochemistry Practical NO (1): Lab Safety

  • This practical focuses on lab safety procedures
  • Students must adhere to safety guidelines before, during, and after lab work.

Preparing for Laboratory Work

  • Before working in the lab, familiarize yourself with:
    • Hazards of lab materials
    • Material labels
    • Proper handling procedures

Throughout Laboratory Work

  • Obey these guidelines:
    • Children are not allowed in the lab
    • Avoid eating, drinking, smoking, using cosmetics, or handling contact lenses.
    • Wear a closed, clean lab coat (knee-length) at all times.
    • Do not wear open-toed shoes like sandals.
    • Walk, do not run, in the lab.
    • Keep exits and hallways clear.
    • Only work with materials you've been instructed to use.
    • Remove damaged equipment immediately.
    • Do not leave reagent bottles (full or empty) on the floor.
    • Return unused materials to their original location.
    • Wash the equipment you used.
    • Wash your hands thoroughly.

Hazardous Materials

  • Hazardous materials can cause health issues, injuries, or property damage if not handled correctly.
  • This includes solids, liquids, and gases that can harm people, organisms, property, or the environment.

Special Precautions for Working with Heat or Fire

  • Never leave a lit Bunsen burner or hot object unattended.
  • When an object is removed from the heat, place it where it's shielded from contact to cool down.
  • Inflammable liquids should never be left open, near a naked flame, hot electric element, or electric motor.
  • Use test tube holders to handle hot lab equipment.
  • Always point the open end of a heated container away from yourself and others.
  • Only use Pyrex glassware for heating.
  • Allow hot materials to cool before moving them.
  • Ensure Bunsen burner hoses are tightly connected.

Special Precautions for Working with Chemicals

  • Never taste or touch substances without specific instructions.
  • Never smell substances without specific instructions.
  • Use chemicals only from properly labeled containers.
  • Wash hands after working with chemicals.
  • Do not add water to acid; always add acid to water to dilute it.
  • Mix heat-generating chemicals slowly.

Special Precautions for Working with Electrical Equipment

  • Ensure the area surrounding electrical equipment is dry to avoid accidents.
  • Avoid touching electrical equipment with wet hands.
  • Keep the area around electrical equipment free from flammable materials.
  • Turn off power switches before plugging and unplugging appliances.

Special Precautions for Working with Glassware

  • Familiarize yourself with glassware and equipment in the lab.
  • Do not use broken or chipped glassware.
  • Make sure glassware is clean before use.
  • Do not pick up broken glass with your bare hands; use a pan and a brush.

Special Precautions for Working with Live or Preserved Specimens

  • Treat live specimens gently and follow instructions for proper care.
  • Wash hands after handling live or preserved specimens.
  • Properly mount and support specimens for dissection.
  • Do not cut specimens while holding them in the air.
  • Do not open Petri dishes containing live cultures unless instructed.
  • Use detergents (e.g., 5-10% detol) to sterilize and clean benches, glassware, and equipment.
  • Wear lab coats

Hazard Warning Symbols

  • A system of pictures warns of potential dangers in the lab; these are known as safety symbols.

Toxic

  • Toxic chemicals can cause death if they penetrate the skin, are smelled, or swallowed.

Flammable

  • Flammable substances ignite easily with heat or electricity.

Corrosive

  • Corrosives destroy tissues, especially soft tissues.

Explosives

  • Explosives are extremely dangerous, and need special handling according to lab procedures.

First Aid

  • Immediately follow appropriate first-aid procedures if any injuries occur, for instance:
    • Bleeding control: bandages and cleansing with iodine alcohol mixture
    • Acid or fire burns: wash with water immediately
    • Poisoning: identify the substance and seek appropriate treatment
    • Skin contamination: wash with water to remove contaminants, and potentially soap if insoluble

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