UMST Preparatory College Biochemistry Lab Safety PDF

UMST Preparatory college Department of Biochemistry Practical NO (1) Safety of Lab  Preparing for laboratory work : Before starting to work in a laboratory formalize your self with the following : 1: the hazards of the materials in the lab 2: read labels before handli...

UMST Preparatory college Department of Biochemistry Practical NO (1) Safety of Lab  Preparing for laboratory work : Before starting to work in a laboratory formalize your self with the following : 1: the hazards of the materials in the lab 2: read labels before handling or opening of the materials 3: an appropriate handling for the materials  Throughout a Laboratory work : you must obey the following 1) Children are not permitted in a lab 2) Avoid eating ,drinking ,smoking ,applying cosmetics , handling contact lens 3) All the time ,wear a closed clean lab coat (knee length ) 4) Do not wear an open shoes as sandals 5) Walk do not run in the lab  Throughout a Laboratory work : you must obey the following 6) Keep exits and passageways clear 7) Work only with the materials you have been applied 8) Remove damage apparatus immediately 9) Do not leave the reagent bottles , empty or full, on the floor 10) Return unused materials ,closed to their previous location 11) Wash the apparatus you have been used 12) Wash your hands.  Hazardous materials Hazardous materials are materials that are capable of causing ill health, injury, or death to people, or environmental or property damage if released in an uncontrolled manner or used improperly. Hazardous material is a solid, liquid or gas that is capable of harming people, living organisms, property or the environment  Special precautions for working with heat or fire: 1. Never leave a lighted Bunsen burner of hot object unattended. When an object is removed from the heat & left to cool, it should be placed where it is shielded from contact. 2. Inflammable liquid bottles should not be left open, not dispensed near a naked flame, hot electric element or electric motor. 3. Use test tube holders to handle hot laboratory equipment. 4. When you are heating something in a container such as a test tube, always point the open end of the container away from yourself & others. 5. Use only Pyrex glassware’s for heating. 6. Allow hot materials to cool before moving them from your lab station. 7. Make sure that Bunsen burner hoses fit tightly.  Special precautions for working with chemicals 1. Never taste or touch substances in the laboratory without specific instructions. 2. Never smell substances in the laboratory without specific instructions. 3. Use materials only from containers that are properly labeled. 4. Wash your hand after working with chemicals. 5. Do not add water to acid. Instead, dilute the acid by adding it to water. 6. Mix heat generating chemicals slowly.  Special precautions for working with electrical equipment. 1. Make sure the area under & around the electrical equipment is dry. 2. Never touch electrical equipment with wet hands. 3. Make sure the area surrounding the electrical equipment is free of flammable materials. 4. Turn off all power switches before plugging an appliance into an outlet  Special Precaution for working with Glassware’s and other laboratory equipments. 1. Become familiar with the names and appearance of all the laboratory equipment’s you will use. 2. Never use broken or chipped glassware. 3. Make sure that all glassware’s are clean before you using it. 4. Do not pick up broken glass with your bare hands. Use a pan and a brush.  Special precautions for working with live or preserved specimens. 1. If live animals are used treat them gently. Follow instructions for their proper care. 2. Always wash your hands after working with live or preserved organisms. 3. Specimens for dissection should be properly mounted and supported. Do not try to cut a specimen while holding it in the air. 4. Do not open Petri dishes containing live cultures unless you are directed to do so. 5. Detergents (detol 5 – 10%) should be used to sterilize and clean benches, glassware and equipment. 6. Lab coats should be worn during the work in the lab.  Hazards warning symbols What are Laboratory Safety Symbols? In order to identify potential hazards quickly, a system of pictures was created to warn people of possible danger.  Toxic chemicals causes death if they penetrate the skin ,smell or swollen via the mouth  Flammable Will burn if it put near afire or electricity  Corrosive Destroyed tissues , especially soft tissue  First Aid 1. Injuries: bleeding should be reduced using bandages; the wound should be cleaned with iodine alcohol mixture, and wrapped with sterile bandage. 2. Acid and fire burns: body burns must be washed immediately with tap water. Eye burns must be washed using eye washer, special cream for burns can be used. 3. Poisoning: if any toxic chemical is swallowed, the mouth must be sensed with water, in case of acid, milk is drunk, in case of alkaline, diluted acetic acid ( vinegar) can be used. 4. Skin contamination requires washing with water and removal of contaminated clothing, if the contaminant is insoluble in water remove with soap and water.