Biochemistry: Enzymes Lecture 9

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What is the role of enzymes in biological reactions?

They lower the activation energy for reactions

What is a holoenzyme?

A complete, functional enzyme with its cofactors

Where does the substrate bind and catalysis occur in enzymes?

Active site

What is the term for the energy barrier that substrates need to overcome before a reaction will occur?

Activation energy

What happens to the activation energy required when enzymes are present?

It decreases

Do enzymes participate directly in the reaction?

No, they do not

Which type of enzymatic action describes the process of joining two or more substrate molecules together?

Ligation

What are small organic molecules that act as cofactors for enzymes called?

Coenzymes

Which type of enzymatic specificity refers to the enzyme acting on a particular steric or optical isomer?

Stereochemical specificity

Which model of enzyme binding suggests that the active site of the enzyme is complementary in shape to the substrate, with no change in shape upon binding?

Lock-and-key model

What family of enzymes assists in the breakdown of carbohydrates?

Amylases

What term is used for cofactors that are tightly bound to enzymes?

Prosthetic groups

Which type of specificity refers to the enzyme acting on molecules with specific functional groups?

Group specificity

What are the most common cofactors?

Coenzymes and metal ions

Which enzyme is used to break protein down into peptides in the stomach?

Pepsin

What term is used for enzymes that contain tightly bound metal ions?

Metalloenzymes

Learn about enzymes, holoenzymes, substrates, and the catalytic role of enzymes in biological reactions. This lecture provides an introduction to the functions and structure of enzymes, presented by Dr. Michael Mounir from the Faculty of Science at Helwan University.

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