Biochemistry, Biotechnology and Techniques

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Questions and Answers

Which technique is most appropriate for separating proteins based on their size and charge?

  • Chromatography
  • Centrifugation
  • Electrophoresis (correct)
  • Spectroscopy

What is the primary difference in chromosomal organization between prokaryotes and eukaryotes?

  • Prokaryotic chromosomes are associated with histone proteins, unlike eukaryotic chromosomes.
  • Prokaryotes have multiple linear chromosomes, while eukaryotes have a single circular chromosome.
  • Eukaryotes lack telomeres, which are present in prokaryotic chromosomes.
  • Eukaryotes have membrane-bound organelles containing chromosomes; prokaryotes do not. (correct)

During cellular respiration, which process directly generates the most ATP?

  • Electron Transport System (correct)
  • Glycolysis
  • TCA cycle
  • Oxidation of fatty acids

In recombinant DNA technology, what role does a vector (e.g., plasmid) typically play?

<p>Carrying foreign DNA into a host cell for replication. (B)</p> Signup and view all the answers

Which of the following is NOT a typical application of biotechnology in agriculture?

<p>Synthesis of artificial fertilizers (C)</p> Signup and view all the answers

How do antimicrobial agents primarily function against microbes?

<p>By disrupting cell wall synthesis or other essential processes. (C)</p> Signup and view all the answers

What is the significance of 'crossing over' in the context of molecular genetics?

<p>It increases genetic variation by exchanging genetic material between homologous chromosomes. (A)</p> Signup and view all the answers

Which process is most directly affected by photoperiodism in plants?

<p>Flowering (C)</p> Signup and view all the answers

What role does vernalization play in certain plant species?

<p>It induces flowering after exposure to prolonged cold. (C)</p> Signup and view all the answers

What is the fundamental role of apoptosis in animal development and health?

<p>To eliminate damaged or unnecessary cells in a controlled manner. (C)</p> Signup and view all the answers

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Flashcards

Chromatography

Separation technique based on differential partitioning of molecules between a mobile and stationary phase.

Spectroscopy

An analytical technique that measures the interaction of electromagnetic radiation with matter.

Microscopy

An instrument used to view small objects or details of objects.

Electrophoresis

Separates molecules based on their size and charge using an electric field.

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Centrifugation

Separation of molecules based on their size and density using centrifugal force.

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Blotting

A technique used to transfer proteins, DNA, or RNA onto a membrane.

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PCR

A technique used to amplify a specific DNA segment.

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Chromatin

The structure that contains the cell's genetic material

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Centromere

Region of a chromosome to which the microtubules of the spindle attach, via the kinetochore, during cell division.

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Biotechnology

The combined disciplines of biochemistry, molecular biology and genetics used to create new and innovative technologies.

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Study Notes

  • The question paper includes 75 questions.
  • All questions are subject-specific.
  • All questions are compulsory.
  • The test is bilingual in English and Hindi.

Techniques

  • Chromatography, spectroscopy, microscopy, electrophoresis, centrifugation, blotting, PCR, and radioisotope techniques are used

Chromatin Structure and Function

  • Chromosome organization in prokaryotes and eukaryotes.
  • Chromatin types, centromeres, telomeres, and the gene concept.

Biochemistry

  • Focus on the structure and functions of proteins, DNA, carbohydrates, lipids, and vitamins.
  • Important reactions are bioenergetics, glycolysis, the TCA cycle, electron transport system, and ATP synthesis.
  • Important processes are fatty acid oxidation and synthesis
  • Membrane structure and function.

Biotechnology

  • Recombinant DNA technology, gene cloning principles, and applications in medicine.
  • Biotechnology applications in industry, agriculture, animal and plant cell culture.
  • Environmental biotechnology.

Microbiology

  • Microbial diversity and bacterial reproduction methods.
  • Antimicrobial agents.
  • Significance of microbes in industry and agriculture.
  • Antigen, antibody, complement systems, immunity, and vaccines.
  • Plant and animal viruses
  • Environmental microbiology.

Molecular Genetics

  • Principles of inheritance, linkage, and crossing over.
  • Chromosomal aberrations and extrachromosomal inheritance.
  • Replication, transcription, and translation.
  • DNA repair and population genetics.

Plant Sciences

  • Includes Bryophytes, Pteridophytes, Gymnosperms, and Angiosperms.
  • Plant vascular systems and economic importance.
  • Photosynthesis, photoperiodism, vernalization, and biogeochemical cycles.

Animal Sciences

  • Characteristics of invertebrates and vertebrates.
  • Focus on Human anatomy, physiology, nerve impulse transmission, and endocrinology.
  • Human diseases, apoptosis, cancer, inherited diseases, and animal cell culture.

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