Podcast
Questions and Answers
What is the rate limiting step of DNA synthesis?
What is the rate limiting step of DNA synthesis?
- Regeneration of tetrahydrofolate
- Formation of dCTP
- Synthesis of dTMP (correct)
- Conversion of dUDP to dTMP
Which enzyme is inhibited by Methotrexate?
Which enzyme is inhibited by Methotrexate?
- Dihydrofolate reductase (correct)
- Thymidylate synthase
- RR enzyme
- Methylene tetrahydrofolate
What is the role of methylene tetrahydrofolate in DNA synthesis?
What is the role of methylene tetrahydrofolate in DNA synthesis?
- It is a substrate for DNA synthesis
- It acts as a methyl donor (correct)
- It is a byproduct of DNA synthesis
- It acts as a competitive inhibitor of thymidylate synthase
What is the purpose of the folate cycle?
What is the purpose of the folate cycle?
What is the effect of 5-Fluorouracil on DNA synthesis?
What is the effect of 5-Fluorouracil on DNA synthesis?
Which of the following compounds is a byproduct of the conversion of dUMP to dTMP?
Which of the following compounds is a byproduct of the conversion of dUMP to dTMP?
What is the role of thymidylate synthase in DNA synthesis?
What is the role of thymidylate synthase in DNA synthesis?
Which of the following is NOT a nucleoside diphosphate used as a substrate for DNA synthesis?
Which of the following is NOT a nucleoside diphosphate used as a substrate for DNA synthesis?
What is the role of dihydrofolate reductase in the folate cycle?
What is the role of dihydrofolate reductase in the folate cycle?
What is the purpose of DHFR in DNA synthesis?
What is the purpose of DHFR in DNA synthesis?
What is the consequence of ADA deficiency?
What is the consequence of ADA deficiency?
What is the role of carbamoyl phosphate synthetase II (CPSII) in pyrimidine synthesis?
What is the role of carbamoyl phosphate synthetase II (CPSII) in pyrimidine synthesis?
What is the effect of elevated orotic acid levels in urine?
What is the effect of elevated orotic acid levels in urine?
What is the purpose of ribonucleotide reductase in pyrimidine synthesis?
What is the purpose of ribonucleotide reductase in pyrimidine synthesis?
What is the consequence of UTP inhibition on CPSII?
What is the consequence of UTP inhibition on CPSII?
What is the consequence of ADA deficiency on immune cells?
What is the consequence of ADA deficiency on immune cells?
What is the purpose of xanthine oxidase inhibitors in treating ADA deficiency?
What is the purpose of xanthine oxidase inhibitors in treating ADA deficiency?
What is the rate-limiting step in the de novo pyrimidine synthesis pathway?
What is the rate-limiting step in the de novo pyrimidine synthesis pathway?
What is the consequence of orotic aciduria?
What is the consequence of orotic aciduria?
What is the purpose of uridine or cytidine treatment in orotic aciduria?
What is the purpose of uridine or cytidine treatment in orotic aciduria?
