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Biochemistry Final Exam: Mechanism of Action

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Biochemistry Final Exam: Mechanism of Action

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Questions and Answers

What is the primary reason for gene expression regulation in terms of energy conservation?

To allocate energy for cell differentiation and development
To maintain internal balance and prevent disease
To reduce energy expenditure on unnecessary protein synthesis (correct)
To enhance response to environmental changes

What type of molecule is a transcription factor protein?

Trans-acting factor (correct)
Repressor protein
Cis-acting factor
Enhancer protein

What is the role of cAMP receptor protein (CRP) in the lac operon?

To inhibit transcription by binding to the regulatory region
To activate enzymes on the lac operon
To stimulate transcription by binding to the promoter
To communicate glucose levels to the promoter (correct)

What is the purpose of chromatin remodeling in gene expression regulation?

To change the structure of chromatin to access or deny access to transcription factors (B) Signup and view all the answers

What is the significance of cis-acting sequences in gene expression regulation?

They are DNA sequences that flanking a gene and influence its expression (C) Signup and view all the answers

What is the net result of positive control in gene expression regulation?

Stimulation of transcription by binding to the promoter (A) Signup and view all the answers

What is the primary target of alpha-amanitin?

DNA pol II (D) Signup and view all the answers

What is the mechanism of action of diphtheria toxin?

ADP-ribosylation of eEF2 (D) Signup and view all the answers

What is the primary target of ciprofloxacin?

DNA gyrase (A) Signup and view all the answers

How does chloramphenicol inhibit protein synthesis?

By binding to the 50S subunit of the bacterial ribosome (B) Signup and view all the answers

What is the primary mechanism of action of ampicillin?

Inhibition of bacterial cell wall synthesis (D) Signup and view all the answers

What is the primary target of methotrexate?

Dihydrofolate reductase (DHFR) (C) Signup and view all the answers