Biochemistry Final Exam: Mechanism of Action
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Questions and Answers

What is the primary reason for gene expression regulation in terms of energy conservation?

  • To allocate energy for cell differentiation and development
  • To maintain internal balance and prevent disease
  • To reduce energy expenditure on unnecessary protein synthesis (correct)
  • To enhance response to environmental changes
  • What type of molecule is a transcription factor protein?

  • Trans-acting factor (correct)
  • Repressor protein
  • Cis-acting factor
  • Enhancer protein
  • What is the role of cAMP receptor protein (CRP) in the lac operon?

  • To inhibit transcription by binding to the regulatory region
  • To activate enzymes on the lac operon
  • To stimulate transcription by binding to the promoter
  • To communicate glucose levels to the promoter (correct)
  • What is the purpose of chromatin remodeling in gene expression regulation?

    <p>To change the structure of chromatin to access or deny access to transcription factors</p> Signup and view all the answers

    What is the significance of cis-acting sequences in gene expression regulation?

    <p>They are DNA sequences that flanking a gene and influence its expression</p> Signup and view all the answers

    What is the net result of positive control in gene expression regulation?

    <p>Stimulation of transcription by binding to the promoter</p> Signup and view all the answers

    What is the primary target of alpha-amanitin?

    <p>DNA pol II</p> Signup and view all the answers

    What is the mechanism of action of diphtheria toxin?

    <p>ADP-ribosylation of eEF2</p> Signup and view all the answers

    What is the primary target of ciprofloxacin?

    <p>DNA gyrase</p> Signup and view all the answers

    How does chloramphenicol inhibit protein synthesis?

    <p>By binding to the 50S subunit of the bacterial ribosome</p> Signup and view all the answers

    What is the primary mechanism of action of ampicillin?

    <p>Inhibition of bacterial cell wall synthesis</p> Signup and view all the answers

    What is the primary target of methotrexate?

    <p>Dihydrofolate reductase (DHFR)</p> Signup and view all the answers

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