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Biochemical Reactions in Microbiology
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Biochemical Reactions in Microbiology

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Questions and Answers

What is the purpose of the Voges Proskaur's reaction (VP) test?

  • To identify bacteria that can consume citrate as a carbon source
  • To detect the fermentation of glucose with the production of acid
  • To differentiate between Shigella spp. and Escherichia coli
  • To detect the production of acetyl methyl carbinol (correct)

    • How is a positive result indicated in the Citrate test?

  • Green color change
  • Red color change
  • Blue color change (correct)
  • Yellow color change

    • What is the outcome of a positive Oxidase test?

  • Deep purple color change (correct)
  • No color change
  • Yellow color change
  • Red color change

    • What does the Catalase test detect in bacteria?

    <p>Production of gas bubbles (O2)</p> Signup and view all the answers

    How does the Coagulase test determine a positive result?

    <p>Conversion of fibrinogen to fibrin</p> Signup and view all the answers

    What is the purpose of the Kovac's reagent in the detection of indole?

    <p>To turn the solution red in the presence of indole</p> Signup and view all the answers

    Which tube result indicates positive acid and gas production in sugar fermentation?

    <p>Tube 3A: Positive acid/Positive gas</p> Signup and view all the answers

    What happens when H2S reacts with peptonized iron or ferrous sulfate in SIM media?

    <p>Black precipitate is produced</p> Signup and view all the answers

    How is the production of indole detected in the biochemical reaction?

    <p>By the appearance of a red ring on the surface</p> Signup and view all the answers

    What does a positive acid and gas result indicate in sugar fermentation?

    <p>Organisms ferment sugar with production of acid and gas</p> Signup and view all the answers

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