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Biochemical Reactions in Microbiology

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Questions and Answers

What is the purpose of the Voges Proskaur's reaction (VP) test?

To identify bacteria that can consume citrate as a carbon source

To detect the fermentation of glucose with the production of acid

To differentiate between Shigella spp. and Escherichia coli

To detect the production of acetyl methyl carbinol (correct)

How is a positive result indicated in the Citrate test?

Green color change

Red color change

Blue color change (correct)

Yellow color change

What is the outcome of a positive Oxidase test?

Deep purple color change (correct)

No color change

Yellow color change

Red color change

What does the Catalase test detect in bacteria?

Production of gas bubbles (O2) (B) Signup and view all the answers

How does the Coagulase test determine a positive result?

Conversion of fibrinogen to fibrin (C) Signup and view all the answers

What is the purpose of the Kovac's reagent in the detection of indole?

To turn the solution red in the presence of indole (C) Signup and view all the answers

Which tube result indicates positive acid and gas production in sugar fermentation?

Tube 3A: Positive acid/Positive gas (B) Signup and view all the answers

What happens when H2S reacts with peptonized iron or ferrous sulfate in SIM media?

Black precipitate is produced (B) Signup and view all the answers

How is the production of indole detected in the biochemical reaction?

By the appearance of a red ring on the surface (B) Signup and view all the answers

What does a positive acid and gas result indicate in sugar fermentation?

Organisms ferment sugar with production of acid and gas (D) Signup and view all the answers