Bio-FET Technology

A Bio-FET consists of two main compartments: one is the biological recognition element and the other is the metal gate.

In a Bio-FET, the metal gate is replaced by an ion-sensitive membrane, electrolyte solution, and reference electrode.

The change in conductance of the FET channel in a Bio-FET is due to the binding of neutral molecules to the gate.

A SARS-CoV-2 biosensor with a Graphene transistor has a channel surface functionalized with spike protein antibody.

The BioFET structure is primarily based on a type of field-effect transistor known as P-N junction transistor.

The amount of detected protein in a SARS-CoV-2 biosensor with a Graphene transistor is inversely proportional to the variation in the transistor channel.

The Nyquist plot represents the impedance of the electrode divided into an internal SAM layer and external biomolecules.

The equivalent circuit for the Nyquist plot consists of four circuit elements: Solution resistance, constant phase elements CPE1, constant resistance Ra, and charge transfer resistance Rct.

The Bio-FET is a field-effect transistor that is gated by changes in the surface potential induced by the binding of molecules.

The FET channel in a biosensor MOSFET is not affected by biological recognition elements.

An ion-sensitive field-effect transistor (ISFET) is also known as a biosensor field-effect transistor (Bio-FET).

SARS-CoV-2 biosensors typically do not involve metal-oxide-semiconductor field-effect transistors (MOSFETs).

The impedance profile of Zo at low frequencies tends to that of ZW at decreasing frequencies.

A thicker film results in a higher frequency at which the curvature of the impedance spectrum is observed.

Equivalent circuit models are not used to interpret EIS data.

The dc current flow within the film increases with increasing film thickness.

The FET channel is directly related to Electrochemical Impedance Spectroscopy.

Metal-oxide-semiconductor field-effect transistors are a type of ion-sensitive field-effect transistors.