BIO 201: Introduction to Biotechnology
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Questions and Answers

What is the primary function of a bioreactor?

  • To grow microorganisms for producing desired products (correct)
  • To extract enzymes from microorganisms
  • To convert biomass into ethanol
  • To separate enzymes from the rest of the mixture
  • Which of the following enzymes is used in the dairy industry?

  • Protease
  • Rennin (correct)
  • Amylases
  • Lipases
  • What is the significance of enzymes being able to function in vitro?

  • They can only be used in living cells
  • They are not useful for industrial applications
  • They can be extracted from cells and used for various purposes (correct)
  • They are only found in microorganisms
  • In which industry are enzymes from barley used?

    <p>Brewing industry</p> Signup and view all the answers

    What is the purpose of amylases in the starch industry?

    <p>To convert starch into glucose</p> Signup and view all the answers

    Which of the following industries uses enzymes from dog and pigeon waste?

    <p>Leather industry</p> Signup and view all the answers

    What is the role of protease in biological detergents?

    <p>To break down proteins</p> Signup and view all the answers

    Which of the following enzymes is used in the baking industry?

    <p>Alpha-amylases</p> Signup and view all the answers

    What is the significance of enzymes being able to function in vitro?

    <p>They can be extracted and used for various purposes</p> Signup and view all the answers

    In which industry are lipases used?

    <p>Dairy industry</p> Signup and view all the answers

    Study Notes

    What is Biotechnology?

    • Biotechnology is the manipulation of living organisms and organic material to serve human needs, covering many diverse applications
    • Examples of biotechnology include yeast in bread and alcohol production, using bacteria to kill other harmful organisms, cloning, and genetic engineering
    • Biotechnology involves many different fields and has many exciting benefits, such as advancements in pharmaceuticals and selective pesticide technology

    Bangladesh and Biotechnology

    • In 2013, Bangladesh commercialized Bt eggplant, becoming the first developing country to take a public sector genetically modified food crop to benefit smallholder farmers
    • Bt eggplant carries a gene that makes it resistant to the main crop pest, fruit and shoot borer
    • Bt eggplant enables farmers to dramatically reduce their use of insecticides by 80% or more, protecting their health and the local environment

    Biomass in Biotechnology

    • Biomass is renewable organic materials that come from plants and animals, such as wood, agricultural crops or wastes, and municipal wastes
    • Biomass is used to produce energy by direct burning or processing into biofuels such as ethanol and methane
    • Feedstock is raw material that supplies machines in industry

    Genetic Engineering in Biotechnology

    • Genetic engineering is a modern modification and subspecialty of biotechnology
    • It deals with the specific and targeted modifications of the genetic material of bacteria, plants, or animals to stimulate them to biosynthesize the desired products of the gene

    Fermentation in Biotechnology

    • Fermentation is the process of converting carbohydrates to alcohol or organic acids using microorganisms (yeasts or bacteria) under anaerobic conditions
    • Fermentation produces energy by anaerobically converting nutrients to alcohols and acids
    • Fermentation has advantages, such as:
      • Producing complex molecules like proteins that cannot be made chemically
      • Having higher percentage yield
      • Not requiring extreme temperatures or pH
      • Having greater specificity due to enzyme-catalyzed reactions
    • Fermentation also has disadvantages, such as:
      • Being contaminated by unwanted microorganisms
      • Having a slower process than chemical processes
      • Requiring handling of large volumes of water
    • Fermentation has various uses, including:
      • Chemical sector: producing ethanol, organic acids, enzymes, and polymers
      • Pharmaceuticals: producing antibiotics, chemical drugs, vaccines, and diagnostics
      • Energy: producing ethanol, methane, and biomass
      • Food: producing dairy products, beverages, baking, and high-fructose syrup
      • Agriculture: producing animal feed, microbial pesticides, and plant cell and tissue culture

    Bioreactor

    • A bioreactor is a large vessel in which microorganisms are grown to produce a desired product
    • The desired product is separated from the rest of the mixture

    Enzyme Technology

    • Enzymes are only found in living cells but can be extracted and used for various purposes
    • Enzymes can keep functioning in vitro, even when separated from their cells
    • Enzymes can be used in various applications, including:
      • Biological detergents: using protease and amylases
      • Baking industry: using alpha-amylases and proteases
      • Brewing industry: using enzymes from barley
      • Dairy industry: using rennin, lipases, and lactases
      • Starch industry: using amylases and glucose isomerase
      • Textile industry: using amylases
      • Leather industry: using enzymes from dog and pigeon waste, and trypsin

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    Description

    This quiz covers the basics of biotechnology, including its definition, applications, and techniques such as genetic engineering, fermentation, and enzyme technology. It is based on Smith's chapter 1-5.

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