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_______ barked
_______ barked
dog
The _______ meowed
The _______ meowed
cat
BIO 121 - Industrial Microbiology University of the Philippines _______ | Basic Techniques in Isolating, Purifying, Screening, and Identifying Microorganisms
BIO 121 - Industrial Microbiology University of the Philippines _______ | Basic Techniques in Isolating, Purifying, Screening, and Identifying Microorganisms
Baguio
Basic Isolation and Cultivation Techniques any natural or man-made _______ environment.
Basic Isolation and Cultivation Techniques any natural or man-made _______ environment.
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Liquid Media Selective media, inhibitors, or enriched media. Used widely for _______.
Liquid Media Selective media, inhibitors, or enriched media. Used widely for _______.
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Serial dilution on To reduce the number of microbial species for plating techniques. Decide the best concentration and solvent to use. Detrimental to rare _______.
Serial dilution on To reduce the number of microbial species for plating techniques. Decide the best concentration and solvent to use. Detrimental to rare _______.
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Filtration separates particulate contents according to ___ and shape.
Filtration separates particulate contents according to ___ and shape.
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Membrane filters are made from intertwined organic ___.
Membrane filters are made from intertwined organic ___.
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Optical tweezers use a focused light beam to attract the medium towards the ___ of the beam.
Optical tweezers use a focused light beam to attract the medium towards the ___ of the beam.
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Micropipette is used for single cell isolation to avoid cell damage and ensure ___ isolation.
Micropipette is used for single cell isolation to avoid cell damage and ensure ___ isolation.
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Phototaxis is utilized for the isolation of flagellates that exhibit ___
Phototaxis is utilized for the isolation of flagellates that exhibit ___
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Flow cytometry is used to count and analyze optical properties of ___
Flow cytometry is used to count and analyze optical properties of ___
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Agar matri pre-inoculated marsbes Principle: Cultivation of microbial cells in the chambers is made possible through diffusion of nutrients from the environment/ liquid media to the chambers.in situ cultivation encourage microbial growth requiring interspecific/intraspecific interaction. 9.01.m prevens prey of arbome contaminaras centrare pream way BIO 121 - Industrial Microbiology University of the Philippines ______ | 1.Soil slurry is prepared in inverted TCI with 3g non-sterile soil and water. 3. 2.Diluted soil filtered into 0.2 um PCM.PCM placed above sterile 0.02 um anopore membrane fixed withing soil slurry TCL.bactera 4.Incubate for 10 days at 22C. 5. 8hr rRNa enrichment on 1/10 TSA agar 6.Fluorescence microscopy of mCFUS after total bacterial staining or FISH.Hollow-fiber membrane Chamber (HFCM) Diffusion Chamber Principle: Incubation of diffusion chamber in situ to allow diffusion of nutrient from environment All Agar mata aquanum, reactor or natural environment 043 pm membrane Syringe BAHA Hollow fiber membrane inoculation of mix environmental cells A.Diffusion Chamber B.
Agar matri pre-inoculated marsbes Principle: Cultivation of microbial cells in the chambers is made possible through diffusion of nutrients from the environment/ liquid media to the chambers.in situ cultivation encourage microbial growth requiring interspecific/intraspecific interaction. 9.01.m prevens prey of arbome contaminaras centrare pream way BIO 121 - Industrial Microbiology University of the Philippines ______ | 1.Soil slurry is prepared in inverted TCI with 3g non-sterile soil and water. 3. 2.Diluted soil filtered into 0.2 um PCM.PCM placed above sterile 0.02 um anopore membrane fixed withing soil slurry TCL.bactera 4.Incubate for 10 days at 22C. 5. 8hr rRNa enrichment on 1/10 TSA agar 6.Fluorescence microscopy of mCFUS after total bacterial staining or FISH.Hollow-fiber membrane Chamber (HFCM) Diffusion Chamber Principle: Incubation of diffusion chamber in situ to allow diffusion of nutrient from environment All Agar mata aquanum, reactor or natural environment 043 pm membrane Syringe BAHA Hollow fiber membrane inoculation of mix environmental cells A.Diffusion Chamber B.
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Soil slurry is prepared in ______ TCI with 3g non-sterile soil and water. 3. 2.Diluted soil filtered into 0.2 um PCM.PCM placed above sterile 0.02 um anopore membrane fixed withing soil slurry TCL.bactera 4.Incubate for 10 days at 22C. 5. 8hr rRNa enrichment on 1/10 TSA agar 6.Fluorescence microscopy of mCFUS after total bacterial staining or FISH.Hollow-fiber membrane Chamber (HFCM) Diffusion Chamber Principle: Incubation of diffusion chamber in situ to allow diffusion of nutrient from environment All Agar mata aquanum, reactor or natural environment 043 pm membrane Syringe BAHA Hollow fiber membrane inoculation of mix environmental cells A.Diffusion Chamber B.
Soil slurry is prepared in ______ TCI with 3g non-sterile soil and water. 3. 2.Diluted soil filtered into 0.2 um PCM.PCM placed above sterile 0.02 um anopore membrane fixed withing soil slurry TCL.bactera 4.Incubate for 10 days at 22C. 5. 8hr rRNa enrichment on 1/10 TSA agar 6.Fluorescence microscopy of mCFUS after total bacterial staining or FISH.Hollow-fiber membrane Chamber (HFCM) Diffusion Chamber Principle: Incubation of diffusion chamber in situ to allow diffusion of nutrient from environment All Agar mata aquanum, reactor or natural environment 043 pm membrane Syringe BAHA Hollow fiber membrane inoculation of mix environmental cells A.Diffusion Chamber B.
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Incubate for 10 days at ______. 5. 8hr rRNa enrichment on 1/10 TSA agar 6.Fluorescence microscopy of mCFUS after total bacterial staining or FISH.Hollow-fiber membrane Chamber (HFCM) Diffusion Chamber Principle: Incubation of diffusion chamber in situ to allow diffusion of nutrient from environment All Agar mata aquanum, reactor or natural environment 043 pm membrane Syringe BAHA Hollow fiber membrane inoculation of mix environmental cells A.Diffusion Chamber B.
Incubate for 10 days at ______. 5. 8hr rRNa enrichment on 1/10 TSA agar 6.Fluorescence microscopy of mCFUS after total bacterial staining or FISH.Hollow-fiber membrane Chamber (HFCM) Diffusion Chamber Principle: Incubation of diffusion chamber in situ to allow diffusion of nutrient from environment All Agar mata aquanum, reactor or natural environment 043 pm membrane Syringe BAHA Hollow fiber membrane inoculation of mix environmental cells A.Diffusion Chamber B.
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Hollow-fiber membrane Chamber (HFCM) ______ Chamber Principle: Incubation of diffusion chamber in situ to allow diffusion of nutrient from environment All Agar mata aquanum, reactor or natural environment 043 pm membrane Syringe BAHA Hollow fiber membrane inoculation of mix environmental cells A.______ Chamber B.
Hollow-fiber membrane Chamber (HFCM) ______ Chamber Principle: Incubation of diffusion chamber in situ to allow diffusion of nutrient from environment All Agar mata aquanum, reactor or natural environment 043 pm membrane Syringe BAHA Hollow fiber membrane inoculation of mix environmental cells A.______ Chamber B.
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Hollow-fiber membrane Chamber (HFCM) Diffusion Chamber Principle: Incubation of diffusion chamber in situ to allow diffusion of nutrient from ______ All Agar mata aquanum, reactor or natural ______ 043 pm membrane Syringe BAHA Hollow fiber membrane inoculation of mix ______al cells A.Diffusion Chamber B.
Hollow-fiber membrane Chamber (HFCM) Diffusion Chamber Principle: Incubation of diffusion chamber in situ to allow diffusion of nutrient from ______ All Agar mata aquanum, reactor or natural ______ 043 pm membrane Syringe BAHA Hollow fiber membrane inoculation of mix ______al cells A.Diffusion Chamber B.
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Hollow-fiber membrane Chamber (HFCM) Diffusion Chamber Principle: Incubation of diffusion chamber in situ to allow diffusion of nutrient from environment All Agar mata aquanum, reactor or natural environment 043 pm membrane Syringe BAHA Hollow fiber membrane inoculation of ______ environmental cells A.Diffusion Chamber B.
Hollow-fiber membrane Chamber (HFCM) Diffusion Chamber Principle: Incubation of diffusion chamber in situ to allow diffusion of nutrient from environment All Agar mata aquanum, reactor or natural environment 043 pm membrane Syringe BAHA Hollow fiber membrane inoculation of ______ environmental cells A.Diffusion Chamber B.
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Microbial Trap 0.03 membrane Cover Agar matric NOT ar-hooded 0.33mmenbrane 02 pm membran C membrane prevencs every of abone -02 zbrane alows actromycetes FIGURE Design and agylic of diffon chamber (A) and acrobal trap (B) Explanations w the text IChip similar with diffusion chamber contains hundreds of chambers to accommodate one cell each to produce pure cultures A.Y. 2023-2024 1st Semester Live-Fluorescent in situ hybridization (FISH) Principle: Labelled DNA probes target rRNA of specific taxonomic groups thru natural or chemical transformation or electroporation.commonly with fluorescence-activated cell sorting (FACS) cell sorting.Reverse Genomics combined Principle: Utilization of engineered genome- informed antibody to capture specific microorganisms from complex microbial communities.Antibody-isolated cells would be cultivated in high-throughput media selections.Things to consider during isolation: 1.Aseptic techniques 2.Growth media 3.Cultivation conditions.Purification of Isolates Plating Moist Chamber Culture Separation by Size (______) Filtration Differential Centrifugation Sonication and Vortexing Dilution Agar Plating Micropipette Checking of Purified Isolates Microscopy O Gross Morphological observation Gram Staining - - Lactophenol Blue Staining simple staining 12 Phenol: kill microorganism Lactic acid: preserve fungal structure.Cotton blue: stain chitin cell walls.Screening of Microorganisms with Bioactivity 9926 Office HBWV
Microbial Trap 0.03 membrane Cover Agar matric NOT ar-hooded 0.33mmenbrane 02 pm membran C membrane prevencs every of abone -02 zbrane alows actromycetes FIGURE Design and agylic of diffon chamber (A) and acrobal trap (B) Explanations w the text IChip similar with diffusion chamber contains hundreds of chambers to accommodate one cell each to produce pure cultures A.Y. 2023-2024 1st Semester Live-Fluorescent in situ hybridization (FISH) Principle: Labelled DNA probes target rRNA of specific taxonomic groups thru natural or chemical transformation or electroporation.commonly with fluorescence-activated cell sorting (FACS) cell sorting.Reverse Genomics combined Principle: Utilization of engineered genome- informed antibody to capture specific microorganisms from complex microbial communities.Antibody-isolated cells would be cultivated in high-throughput media selections.Things to consider during isolation: 1.Aseptic techniques 2.Growth media 3.Cultivation conditions.Purification of Isolates Plating Moist Chamber Culture Separation by Size (______) Filtration Differential Centrifugation Sonication and Vortexing Dilution Agar Plating Micropipette Checking of Purified Isolates Microscopy O Gross Morphological observation Gram Staining - - Lactophenol Blue Staining simple staining 12 Phenol: kill microorganism Lactic acid: preserve fungal structure.Cotton blue: stain chitin cell walls.Screening of Microorganisms with Bioactivity 9926 Office HBWV
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Live-Fluorescent in situ hybridization (FISH) Principle: Labelled DNA probes target rRNA of specific taxonomic groups thru natural or chemical transformation or electroporation.commonly with fluorescence-activated cell sorting (FACS) cell sorting.Reverse Genomics combined Principle: Utilization of engineered genome- informed antibody to capture specific microorganisms from complex microbial communities.Antibody-isolated cells would be cultivated in high-throughput media selections.Things to consider during isolation: 1.Aseptic techniques 2.Growth media 3.Cultivation conditions.Purification of Isolates Plating Moist Chamber Culture Separation by Size (Microalgae) Filtration Differential Centrifugation Sonication and Vortexing Dilution Agar Plating Micropipette Checking of Purified Isolates Microscopy O Gross Morphological observation Gram Staining - - Lactophenol Blue Staining simple staining 12 Phenol: kill microorganism Lactic acid: preserve fungal structure.Cotton blue: stain chitin cell walls.Screening of Microorganisms with Bioactivity 9926 Office HBWV
Live-Fluorescent in situ hybridization (FISH) Principle: Labelled DNA probes target rRNA of specific taxonomic groups thru natural or chemical transformation or electroporation.commonly with fluorescence-activated cell sorting (FACS) cell sorting.Reverse Genomics combined Principle: Utilization of engineered genome- informed antibody to capture specific microorganisms from complex microbial communities.Antibody-isolated cells would be cultivated in high-throughput media selections.Things to consider during isolation: 1.Aseptic techniques 2.Growth media 3.Cultivation conditions.Purification of Isolates Plating Moist Chamber Culture Separation by Size (Microalgae) Filtration Differential Centrifugation Sonication and Vortexing Dilution Agar Plating Micropipette Checking of Purified Isolates Microscopy O Gross Morphological observation Gram Staining - - Lactophenol Blue Staining simple staining 12 Phenol: kill microorganism Lactic acid: preserve fungal structure.Cotton blue: stain chitin cell walls.Screening of Microorganisms with Bioactivity 9926 Office HBWV
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Reverse Genomics combined Principle: Utilization of engineered genome- informed antibody to capture specific microorganisms from complex microbial communities.Antibody-isolated cells would be cultivated in high-throughput media selections.Things to consider during isolation: 1.Aseptic techniques 2.Growth media 3.Cultivation conditions.Purification of Isolates Plating Moist Chamber Culture Separation by Size (Microalgae) Filtration Differential Centrifugation Sonication and Vortexing Dilution Agar Plating Micropipette Checking of Purified Isolates Microscopy O Gross Morphological observation Gram Staining - - Lactophenol Blue Staining simple staining 12 Phenol: kill microorganism Lactic acid: preserve fungal structure.Cotton blue: stain chitin cell walls.Screening of Microorganisms with Bioactivity 9926 Office HBWV
Reverse Genomics combined Principle: Utilization of engineered genome- informed antibody to capture specific microorganisms from complex microbial communities.Antibody-isolated cells would be cultivated in high-throughput media selections.Things to consider during isolation: 1.Aseptic techniques 2.Growth media 3.Cultivation conditions.Purification of Isolates Plating Moist Chamber Culture Separation by Size (Microalgae) Filtration Differential Centrifugation Sonication and Vortexing Dilution Agar Plating Micropipette Checking of Purified Isolates Microscopy O Gross Morphological observation Gram Staining - - Lactophenol Blue Staining simple staining 12 Phenol: kill microorganism Lactic acid: preserve fungal structure.Cotton blue: stain chitin cell walls.Screening of Microorganisms with Bioactivity 9926 Office HBWV
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Things to consider during isolation: 1.Aseptic techniques 2.Growth media 3.Cultivation conditions.Purification of Isolates Plating Moist Chamber Culture Separation by Size (Microalgae) Filtration Differential Centrifugation Sonication and Vortexing Dilution Agar Plating Micropipette Checking of Purified Isolates Microscopy O Gross Morphological observation Gram Staining - - Lactophenol Blue Staining simple staining 12 Phenol: kill microorganism Lactic acid: preserve fungal structure.Cotton blue: stain chitin cell walls.Screening of Microorganisms with Bioactivity 9926 Office HBWV
Things to consider during isolation: 1.Aseptic techniques 2.Growth media 3.Cultivation conditions.Purification of Isolates Plating Moist Chamber Culture Separation by Size (Microalgae) Filtration Differential Centrifugation Sonication and Vortexing Dilution Agar Plating Micropipette Checking of Purified Isolates Microscopy O Gross Morphological observation Gram Staining - - Lactophenol Blue Staining simple staining 12 Phenol: kill microorganism Lactic acid: preserve fungal structure.Cotton blue: stain chitin cell walls.Screening of Microorganisms with Bioactivity 9926 Office HBWV
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Screening of Microorganisms with Bioactivity 9926 Office HBWV
Screening of Microorganisms with Bioactivity 9926 Office HBWV
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Plating Moist Chamber Culture Separation by Size (Microalgae) Filtration Differential Centrifugation Sonication and Vortexing Dilution Agar Plating Micropipette Checking of Purified Isolates Microscopy O Gross Morphological observation Gram Staining - - Lactophenol Blue Staining simple staining 12 Phenol: kill microorganism Lactic acid: preserve fungal structure.Cotton blue: stain chitin cell walls.Screening of Microorganisms with Bioactivity 9926 Office HBWV
Plating Moist Chamber Culture Separation by Size (Microalgae) Filtration Differential Centrifugation Sonication and Vortexing Dilution Agar Plating Micropipette Checking of Purified Isolates Microscopy O Gross Morphological observation Gram Staining - - Lactophenol Blue Staining simple staining 12 Phenol: kill microorganism Lactic acid: preserve fungal structure.Cotton blue: stain chitin cell walls.Screening of Microorganisms with Bioactivity 9926 Office HBWV
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