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- Agar matri pre-inoculated marsbes Principle: Cultivation of microbial cells in the chambers is made possible through diffusion of nutrients from the environment/ liquid media to the chambers. in situ cultivation encourage microbial growth requiring interspecific/intraspecific interaction. 9.01.m p...

- Agar matri pre-inoculated marsbes Principle: Cultivation of microbial cells in the chambers is made possible through diffusion of nutrients from the environment/ liquid media to the chambers. in situ cultivation encourage microbial growth requiring interspecific/intraspecific interaction. 9.01.m prevens prey of arbome contaminaras centrare pream way BIO 121 - Industrial Microbiology University of the Philippines Baguio | 1. Soil slurry is prepared in inverted TCI with 3g non-sterile soil and water. 3. 2. Diluted soil filtered into 0.2 um PCM. PCM placed above sterile 0.02 um anopore membrane fixed withing soil slurry TCL. bactera 4. Incubate for 10 days at 22C. 5. 8hr rRNa enrichment on 1/10 TSA agar 6. Fluorescence microscopy of mCFUS after total bacterial staining or FISH. Hollow-fiber membrane Chamber (HFCM) Diffusion Chamber Principle: Incubation of diffusion chamber in situ to allow diffusion of nutrient from environment All Agar mata aquanum, reactor or natural environment 043 pm membrane Syringe BAHA Hollow fiber membrane inoculation of mix environmental cells A. Diffusion Chamber B. Microbial Trap 0.03 membrane Cover Agar matric NOT ar-hooded 0.33mmenbrane 02 pm membran C membrane prevencs every of abone -02 zbrane alows actromycetes FIGURE Design and agylic of diffon chamber (A) and acrobal trap (B) Explanations w the text IChip similar with diffusion chamber contains hundreds of chambers to accommodate one cell each to produce pure cultures A.Y. 2023-2024 1st Semester Live-Fluorescent in situ hybridization (FISH) Principle: Labelled DNA probes target rRNA of specific taxonomic groups thru natural or chemical transformation or electroporation. commonly with fluorescence-activated cell sorting (FACS) cell sorting. Reverse Genomics combined Principle: Utilization of engineered genome- informed antibody to capture specific microorganisms from complex microbial communities. Antibody-isolated cells would be cultivated in high-throughput media selections. Things to consider during isolation: 1. Aseptic techniques 2. Growth media 3. Cultivation conditions. Purification of Isolates Plating Moist Chamber Culture Separation by Size (Microalgae) Filtration Differential Centrifugation Sonication and Vortexing Dilution Agar Plating Micropipette Checking of Purified Isolates Microscopy O Gross Morphological observation Gram Staining - - Lactophenol Blue Staining simple staining 12 Phenol: kill microorganism Lactic acid: preserve fungal structure. Cotton blue: stain chitin cell walls. Screening of Microorganisms with Bioactivity 9926 Office HBWV