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Questions and Answers
Which method employs Amido black stain for detecting Bence Jones protein?
Which method employs Amido black stain for detecting Bence Jones protein?
- Modified Coomassie brilliant blue stain
- Immunoassay for free light chains
- Immunofixation electrophoresis
- Protein electrophoresis (correct)
What indicates the presence of Bence Jones globulin during protein electrophoresis?
What indicates the presence of Bence Jones globulin during protein electrophoresis?
- Multiple peaks in the light chain region
- Absence of peaks in the globulin region
- A diffuse pattern in the albumin region
- A single sharp peak in the globulin region (correct)
Under what condition is Bence Jones protein's precipitate likely not to redissolve?
Under what condition is Bence Jones protein's precipitate likely not to redissolve?
- When the protein is too concentrated (correct)
- At temperatures below 40° C
- At temperatures above 60° C
- In the presence of ammonium sulfate
Which method's results may yield false-positive reactions due to other globulins?
Which method's results may yield false-positive reactions due to other globulins?
Which temperature range is associated with the precipitation of Bence Jones protein?
Which temperature range is associated with the precipitation of Bence Jones protein?
Flashcards
Bence Jones protein
Bence Jones protein
A protein found in the urine of patients with multiple myeloma, a type of blood cancer.
Bence Jones Proteinuria Determination
Bence Jones Proteinuria Determination
A laboratory test used to detect the presence of Bence Jones protein in urine.
Protein Electrophoresis
Protein Electrophoresis
A technique for separating proteins based on their size and electrical charge. It's used to identify Bence Jones protein in urine.
Immunoassay for Free Light Chains
Immunoassay for Free Light Chains
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Immunofixation Electrophoresis
Immunofixation Electrophoresis
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Study Notes
Bence Jones Proteinuria Detection Methods
- Several methods detect Bence Jones protein in urine, including electrophoresis, immunofixation electrophoresis, and immunoassay for free light chains.
- Traditional electrophoresis uses Amido black stain on highly concentrated urine (200-fold).
- Newer methods use less concentrated urine and modified Coomassie brilliant blue stain, maintaining sensitivity and specificity.
- Characteristic sign is a single, sharp peak in the globulin region on electrophoresis, indicating Bence Jones globulin or clonal immunoglobulin production.
- Bence Jones globulin consists of either kappa (κ) or lambda (λ) immunoglobulin light chains.
- Bence Jones protein precipitates between 40°C and 60°C and redissolves around 100°C.
- Other methods include cold precipitation with salts (e.g., ammonium sulfate) or acids.
- High levels of Bence Jones protein typically yield positive results across methods.
- Low levels or presence of other globulins may cause unreliable results.
- False positives can arise if other globulins precipitate with heat and acetic acid.
- False negatives may occur with extremely concentrated Bence Jones protein where precipitate does not redissolve with boiling.
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