Bence Jones Proteinuria Detection Methods

Which method employs Amido black stain for detecting Bence Jones protein?

What indicates the presence of Bence Jones globulin during protein electrophoresis?

Under what condition is Bence Jones protein's precipitate likely not to redissolve?

Which method's results may yield false-positive reactions due to other globulins?

Which temperature range is associated with the precipitation of Bence Jones protein?

Bence Jones Proteinuria Detection Methods

Several methods detect Bence Jones protein in urine, including electrophoresis, immunofixation electrophoresis, and immunoassay for free light chains.
Traditional electrophoresis uses Amido black stain on highly concentrated urine (200-fold).
Newer methods use less concentrated urine and modified Coomassie brilliant blue stain, maintaining sensitivity and specificity.
Characteristic sign is a single, sharp peak in the globulin region on electrophoresis, indicating Bence Jones globulin or clonal immunoglobulin production.
Bence Jones globulin consists of either kappa (κ) or lambda (λ) immunoglobulin light chains.
Bence Jones protein precipitates between 40°C and 60°C and redissolves around 100°C.
Other methods include cold precipitation with salts (e.g., ammonium sulfate) or acids.
High levels of Bence Jones protein typically yield positive results across methods.
Low levels or presence of other globulins may cause unreliable results.
False positives can arise if other globulins precipitate with heat and acetic acid.
False negatives may occur with extremely concentrated Bence Jones protein where precipitate does not redissolve with boiling.

Explore the various methods for detecting Bence Jones protein in urine, including traditional and newer techniques like electrophoresis and immunofixation. This quiz covers the principles behind these methods and the significance of detecting Bence Jones globulin. Test your knowledge on the characteristics and precipitation behavior of this unique protein.