Podcast
Questions and Answers
Which method is traditionally used to detect Bence Jones protein in concentrated urine?
Which method is traditionally used to detect Bence Jones protein in concentrated urine?
- Protein electrophoresis with Amido black stain (correct)
- Modified Coomassie brilliant blue stain
- Immunoassay for free light chains
- Immunofixation electrophoresis
What indicates the presence of Bence Jones globulin in protein electrophoresis?
What indicates the presence of Bence Jones globulin in protein electrophoresis?
- Multiple overlapping peaks
- No distinct peaks
- A broad peak in the albumin region
- A single sharp peak in the globulin region (correct)
At what temperature does Bence Jones protein precipitate?
At what temperature does Bence Jones protein precipitate?
- 40° C to 60° C (correct)
- 0° C to 20° C
- Above 100° C
- 20° C to 40° C
Which component may represent either the κ or λ immunoglobulin light chain?
Which component may represent either the κ or λ immunoglobulin light chain?
Which staining method is comparable in sensitivity and specificity to traditional electrophoresis?
Which staining method is comparable in sensitivity and specificity to traditional electrophoresis?
What is one of the newer methods performed on less concentrated urine for detecting Bence Jones protein?
What is one of the newer methods performed on less concentrated urine for detecting Bence Jones protein?
Flashcards
What is Bence Jones protein?
What is Bence Jones protein?
A type of protein found in the urine of patients with multiple myeloma.
What is protein electrophoresis used for in Bence Jones proteinuria?
What is protein electrophoresis used for in Bence Jones proteinuria?
A method used to detect Bence Jones protein in urine, which separates proteins based on their charge and size.
What is immunofixation electrophoresis?
What is immunofixation electrophoresis?
Immunofixation electrophoresis is a technique that combines protein electrophoresis with an immunochemical reaction to identify and quantify specific proteins in a sample.
How are free light chains used in Bence Jones protein detection?
How are free light chains used in Bence Jones protein detection?
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What is the unique temperature behavior of Bence Jones protein?
What is the unique temperature behavior of Bence Jones protein?
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What is the typical pattern of Bence Jones protein on electrophoresis?
What is the typical pattern of Bence Jones protein on electrophoresis?
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Study Notes
Bence Jones Proteinuria Detection Methods
- Bence Jones protein detection methods include protein electrophoresis, immunofixation electrophoresis, and immunoassay for free light chains.
- Traditional electrophoresis uses Amido black stain on 200-fold concentrated urine.
- Newer methods use modified Coomassie brilliant blue stain on less concentrated urine, maintaining sensitivity and specificity.
- A single sharp peak in the globulin region during protein electrophoresis indicates Bence Jones protein or clonal immunoglobulin production.
- Bence Jones globulin is either κ or λ immunoglobulin light chain.
- Bence Jones protein precipitates between 40°C and 60°C and redissolves near 100°C.
- Other methods involve cold precipitation with salts (ammonium sulfate) and acids.
- High levels of Bence Jones proteinuria generally result in positive test outcomes for most methods.
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