Podcast
Questions and Answers
What is a key characteristic of the 'removal and replacement' technique in behavioural endocrinology?
What is a key characteristic of the 'removal and replacement' technique in behavioural endocrinology?
- It relies on observing changes in behavior after administering a hormone agonist.
- It focuses primarily on quantifying hormone receptor densities in different tissues.
- It enables causal interpretations by measuring differences before and after hormone source manipulation. (correct)
- It involves measuring hormone levels indirectly using competitive binding assays.
In the context of an immunoassay, what is the primary purpose of using an animal's immune system?
In the context of an immunoassay, what is the primary purpose of using an animal's immune system?
- To directly measure the concentration of hormones in the animal's blood.
- To generate antibodies that can specifically bind to a substance of interest. (correct)
- To create a standardized solution for hormone replacement therapy.
- To induce a state of hormonal hyper-responsiveness in the animal.
During the radioimmunoassay (RIA) process, what is the significance of using a 'hot ligand'?
During the radioimmunoassay (RIA) process, what is the significance of using a 'hot ligand'?
- It allows for the precise quantification of the cold ligands through radioactive emissions. (correct)
- It ensures complete binding of all antibody sites in the test tube.
- It functions as an antagonist that blocks any binding of cold ligand.
- It acts a control to verify the quantity of the cold ligand.
Why is it important to use increasing concentrations of a 'cold standard' in a radioimmunoassay?
Why is it important to use increasing concentrations of a 'cold standard' in a radioimmunoassay?
In a radioimmunoassay, what occurs when more cold testosterone is added to the test tube?
In a radioimmunoassay, what occurs when more cold testosterone is added to the test tube?
What fundamental principle of the immune system is harnessed in immunoassays?
What fundamental principle of the immune system is harnessed in immunoassays?
Rosalyn Sussman Yalow was awarded the Nobel Prize for which contribution to science?
Rosalyn Sussman Yalow was awarded the Nobel Prize for which contribution to science?
What is a standard curve used for in the context of radioimmunoassays?
What is a standard curve used for in the context of radioimmunoassays?
What is the primary distinction between direct and indirect immunohistochemistry?
What is the primary distinction between direct and indirect immunohistochemistry?
In the context of the provided material, what does an 'agonist' do at a receptor site?
In the context of the provided material, what does an 'agonist' do at a receptor site?
Which of the following techniques is used to visualize hormone receptor locations using a radio labeled hormone?
Which of the following techniques is used to visualize hormone receptor locations using a radio labeled hormone?
The concentration of estradiol is measured in units of picograms per milliliter. This is also equivalent to:
The concentration of estradiol is measured in units of picograms per milliliter. This is also equivalent to:
Which statement about the ventromedial hypothalamus is correct, based on the information provided?
Which statement about the ventromedial hypothalamus is correct, based on the information provided?
What does 'mass percentage' most commonly entail, in the area of endocrinology?
What does 'mass percentage' most commonly entail, in the area of endocrinology?
Caffeine acts at the adenosine receptor as a(n):
Caffeine acts at the adenosine receptor as a(n):
Hormones such as luteinizing hormone (LH) and follicle stimulating hormone (FSH), which are produced by the pituitary gland, are measured in what units?
Hormones such as luteinizing hormone (LH) and follicle stimulating hormone (FSH), which are produced by the pituitary gland, are measured in what units?
Which technique uses radioactive substances to visualize brain activity?
Which technique uses radioactive substances to visualize brain activity?
What is the primary function of the parathyroid hormone (PTH)?
What is the primary function of the parathyroid hormone (PTH)?
What is a characteristic of endocrine glands?
What is a characteristic of endocrine glands?
Which of the following brain imaging techniques would be best for visualizing large brain structures?
Which of the following brain imaging techniques would be best for visualizing large brain structures?
What is used to visualize cells that have recently been active in animal brain imaging?
What is used to visualize cells that have recently been active in animal brain imaging?
Which of the options are NOT a type of brain imaging technique?
Which of the options are NOT a type of brain imaging technique?
Which method uses magnetic fields to measure brain function?
Which method uses magnetic fields to measure brain function?
What is a primary role of calcium in the body as described in this content?
What is a primary role of calcium in the body as described in this content?
Which of the following hormones is primarily responsible for maintaining the corpus luteum during early pregnancy?
Which of the following hormones is primarily responsible for maintaining the corpus luteum during early pregnancy?
What is a primary function of chorionic somatomammotropin (CS) in mammals?
What is a primary function of chorionic somatomammotropin (CS) in mammals?
Which of these steroid hormones is primarily involved in the body's stress response?
Which of these steroid hormones is primarily involved in the body's stress response?
Which of the following is correct about the structure of steroid hormones?
Which of the following is correct about the structure of steroid hormones?
What is a characteristic of steroid hormones that necessitates the use of carrier proteins in the bloodstream?
What is a characteristic of steroid hormones that necessitates the use of carrier proteins in the bloodstream?
Where are progesterone-type hormones produced in the brain?
Where are progesterone-type hormones produced in the brain?
Which of the following hormones is detectable by pregnancy tests?
Which of the following hormones is detectable by pregnancy tests?
What role do androgens play in the human body?
What role do androgens play in the human body?
What is the primary function of Sertoli cells within the testes?
What is the primary function of Sertoli cells within the testes?
Which hormone is directly stimulated by Luteinizing Hormone (LH) in Leydig cells?
Which hormone is directly stimulated by Luteinizing Hormone (LH) in Leydig cells?
During the follicular phase of the ovarian cycle, what is the role of granulosa cells?
During the follicular phase of the ovarian cycle, what is the role of granulosa cells?
What is the primary hormone produced by the corpus luteum?
What is the primary hormone produced by the corpus luteum?
Which of these hormones is primarily responsible for softening pelvic ligaments during pregnancy?
Which of these hormones is primarily responsible for softening pelvic ligaments during pregnancy?
Which of the following is NOT a known effect of testosterone?
Which of the following is NOT a known effect of testosterone?
What effect does inhibin have on hormone secretion?
What effect does inhibin have on hormone secretion?
What is the role of FSH in the ovaries?
What is the role of FSH in the ovaries?
Which pituitary hormone is NOT named after the target it affects?
Which pituitary hormone is NOT named after the target it affects?
What is the primary effect of vasopressin on the kidneys?
What is the primary effect of vasopressin on the kidneys?
Which statement accurately describes the relationship between GnRH and the pituitary hormones?
Which statement accurately describes the relationship between GnRH and the pituitary hormones?
What is the role of heat shock proteins (HSP) in steroid hormone signaling?
What is the role of heat shock proteins (HSP) in steroid hormone signaling?
What is the precursor molecule responsible for the synthesis of ACTH, beta-endorphin, and met-enkephalin?
What is the precursor molecule responsible for the synthesis of ACTH, beta-endorphin, and met-enkephalin?
How does ethanol consumption affect vasopressin release?
How does ethanol consumption affect vasopressin release?
Which of the following best describes the action of steroid hormones within a target cell?
Which of the following best describes the action of steroid hormones within a target cell?
What are the two hormones released by the posterior pituitary?
What are the two hormones released by the posterior pituitary?
What process specifically stimulates the release of oxytocin from the posterior pituitary to initiate milk ejection?
What process specifically stimulates the release of oxytocin from the posterior pituitary to initiate milk ejection?
Which of the following statements is true regarding the intermediate lobe of the pituitary gland?
Which of the following statements is true regarding the intermediate lobe of the pituitary gland?
Flashcards
Removal and Replacement
Removal and Replacement
A scientific technique that involves removing a source of a hormone and measuring changes in the organism before and after removal. This method helps establish a causal relationship between the hormone and its effects.
Immunoassay
Immunoassay
A laboratory test that uses antibodies to detect and quantify specific substances in biological samples, like blood or tissues. This method is widely used in hormone research.
Radioimmunoassay
Radioimmunoassay
A type of immunoassay that uses radioactive labels to detect and quantify substances. This method is particularly useful for studying hormones and their effects.
Competitive Binding
Competitive Binding
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Standard Curve
Standard Curve
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Hot Ligand
Hot Ligand
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Cold Standard
Cold Standard
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Antibody
Antibody
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Immunohistochemistry
Immunohistochemistry
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Direct Detection Immunohistochemistry
Direct Detection Immunohistochemistry
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Indirect Detection Immunohistochemistry
Indirect Detection Immunohistochemistry
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Autoradiography
Autoradiography
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Pharmacological Technique
Pharmacological Technique
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Agonist
Agonist
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Antagonist
Antagonist
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Immediate Early Genes (IEGs)
Immediate Early Genes (IEGs)
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Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
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CT Scan
CT Scan
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MRI
MRI
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fMRI
fMRI
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PET Scan
PET Scan
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Electroencephalogram (EEG)
Electroencephalogram (EEG)
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Thyroid gland
Thyroid gland
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Leptin
Leptin
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Testes
Testes
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Leydig Cells
Leydig Cells
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Sertoli Cells
Sertoli Cells
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Inhibin
Inhibin
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Ovaries
Ovaries
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Follicular Phase
Follicular Phase
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Luteal Phase
Luteal Phase
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Chorionic Gonadotropin (CG)
Chorionic Gonadotropin (CG)
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Chorionic Somatomammotropin (CS), Chorionic Corticotropin (CC), and Chorionic Thyrotropin (CT)
Chorionic Somatomammotropin (CS), Chorionic Corticotropin (CC), and Chorionic Thyrotropin (CT)
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Aldosterone
Aldosterone
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Cortisol
Cortisol
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Androgens
Androgens
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Estrogens and Progesterone
Estrogens and Progesterone
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Progesterone-type hormones
Progesterone-type hormones
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Steroid Hormone Structure
Steroid Hormone Structure
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What are tropic hormones?
What are tropic hormones?
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What is the HPG axis?
What is the HPG axis?
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What is the HPT axis?
What is the HPT axis?
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What is the role of growth hormone?
What is the role of growth hormone?
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What is the role of prolactin?
What is the role of prolactin?
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What is POMC?
What is POMC?
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What is the role of vasopressin?
What is the role of vasopressin?
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What is the role of oxytocin?
What is the role of oxytocin?
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How do steroid hormone receptors function?
How do steroid hormone receptors function?
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Why are the effects of steroid hormones not immediate?
Why are the effects of steroid hormones not immediate?
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Study Notes
Research Techniques in Behavioral Endocrinology
- Removal and replacement: Possible to make causal interpretations. Removal of the hormone source allows researchers to measure changes before and after removal.
Immunoassay
- Way to detect specific biological substances in blood and tissues.
- Routinely used to measure hormone levels.
- A method that uses the principles of the immune system to quantify or visualize specific substances.
- An example is using one animal's immune system to create antibodies to a substance of interest from another animal (e.g., testosterone in rat, injected into rabbit).
- Procedures:
- Take testosterone from a rat and inject it into a rabbit.
- The rabbit's body creates antibodies.
- Take blood from the rabbit.
- Rabbits' antibodies are extracted, purified, and tagged with a detectable label to identify them.
Radioimmunoassay (RIA)
- Type of immunoassay uses radioactivity to detect chemical substances in body tissues.
- Pioneered by Rosalind Sussman Jalloh (Nobel Prize in Physiology or Medicine in 1977).
- Requires a "hot ligand" (radioactively labeled ligand) and a "cold standard" (non-radioactively labeled ligand).
- Known amounts of the cold standard are added to tubes in increasing concentrations.
- Each tube contains the same amount of testosterone antibody.
- The hot and cold ligands compete for binding sites on the antibody.
- The excess fluid is removed, and the amount of radioactivity bound to the antibody is measured.
- More cold testosterone added leads to less binding of the hot testosterone, less radioactivity detected.
- Plotting known concentrations of the cold standards versus the radioactivity produces a standard curve, crucial for determining unknown sample concentrations.
Enzyme-linked Immunosorbent Assay (ELISA)
- Quantifies hormones in various tissues (hair, nails, feces, blubber).
- Uses color intensity as a label (instead of radioactivity).
- Uses small sample quantities.
- Standards of known concentrations generate a standard curve for sample quantification.
- A darker colour indicates a stronger hormone concentration.
Hormone Alteration Experiments
- Experiments to determine if verbal aggression affects testosterone levels.
- Subjects are randomly assigned to insult or neutral conditions.
- Saliva samples are collected.
- ELISA is used for testosterone quantification.
- Intra-assay and inter-assay variations are considered for error prevention.
ELISA Applications
- Pregnancy tests (detecting HCG).
- Early ovulation tests.
- COVID-19 tests.
Hormone Conversions
- Deci (d), centi (c), milli (m), micro (μ), nano (n), pico (p), femto (f). Unit conversion factors for relative concentrations (such as pg/mL, ng/mL) in plasma/serum (often measured as 1mL or 1L).
Hormone Mass Percentage
- The amount of a hormone in 100 mL of plasma or serum.
- Conversion between mass percentage and other units (e.g., pmol/L) is often needed and can be done via referencing conversion factors (available online).
Immunohistochemistry
- Type of immunoassay that visualizes hormone receptors or proteins in tissues by applying labeled antibodies.
- Useful for determining the location and distribution of hormone receptors in the brain.
- Direct detection uses a single antibody targeting the target receptor.
- Indirect detection uses multiple antibodies & large binding proteins (like horseradish peroxidase) for visualization using microscopy techniques.
Autoradiography
- Technique used to locate hormone receptors by injecting a radiolabeled hormone.
- Tissues of interest are then exposed to radioactive material sensitive film to visualize receptor locations in tissues.
- This technique is useful for studying hormone receptor distribution.
Brain Imaging (Humans)
- Non-invasive techniques including CT (X-rays), PET (positron emission), MRI (magnetic fields), fMRI (magnetic fields, BOLD), and EEG (electrical activity) for detecting brain activity and structures.
Brain Imaging (Animals)
- Immunohistochemistry uses immediate early genes (e.g., ZENK, EGR-1, NGFI-A, and C-FOS) after neuron activation for indicating neuronal activity in specific brain areas.
- Zebra finches experiments using NCM (Nucleus for the Control of Memory) to illustrate how auditory signals affect neurons.
Hormone Receptor Localization Techniques
- Summary of appropriate methods (immunoassay/ELISA, immunohistochemistry, autoradiography, human & animal brain imaging) to determine hormone receptor location in the body, noting criteria to decide on the approach like size of target structures.
Hormone Availability Factors
- Factors influencing hormone availability in blood:
- Lipophilicity (affects storage & release),
- Steroid breakdown (catabolism),
- Carrier protein affinity & capability,
- Binding strength to carrier proteins that inhibits hormone release (inactive steroid).
Hypothalamic and Pituitary Hormones
- All hypothalamic and pituitary hormones are peptides or proteins.
- Hormones travel through the portal system in the brain. This pathway involves a capillary network that connects the hypothalamus to the anterior pituitary.
- The hypothalamus releases releasing hormones into the portal system that trigger release of hormones in the anterior pituitary (e.g. GnRH – Gonadotropin-releasing hormone).
- The anterior pituitary then releases hormones that travel via blood to other endocrine organs. The posterior pituitary releases hormones that are produced by the hypothalamus.
Hormone Receptors Types
- Steroid receptors are found inside target cells, enabling direct interaction with DNA for gene transcription regulation.
- Peptide receptors are embedded within the cell membrane, activating intracellular enzymes (via 2nd messengers like cAMP) for response signaling.
- The two major peptide receptor types are enzyme-linked and G-protein coupled.
Hormone Receptor Mechanisms (Steroids & Peptides)
- Mechanisms that explain how steroid hormones directly affect gene expression by binding and influencing DNA transcription.
- Mechanisms of peptide hormone receptor activation, explaining the effects of hormones relying on second messengers (e.g. cAMP).
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