BB50241 (2023-24) Life Sciences Research Project 1A Safety Quiz

EXP11: Fungal DNA extractions

EXP9: Protoplast-mediated transformation of fungi using auxotrophic markers

EXP7: Confirmation CRISPR-Cas9 vector with yA sgRNA guide is error-free

EXP5: USER cloning (correct)

To isolate CRISPR-Cas9 vector with yA sgRNA guide

To evaluate the performance of CRISPR-Cas9 in A. nidulans (correct)

To amplify the NHEJ mutated yA sequence

To confirm the error-free CRISPR-Cas9 vector with yA sgRNA guide

Gel purification of PCRs and linearized vector

Analysis of cloned yA-sgRNA sequence within the pFC330 vector

Protoplast-mediated transformation of fungi using auxotrophic markers

Amplification of NHEJ mutated yA sequence (correct)

<p>Responsible for laboratory health and safety</p> Signup and view all the answers

<p>Isolation of CRISPR-Cas9 containing the yA sgRNA guide and yA gene-targeting substrate vectors</p> Signup and view all the answers

<p>EXP8: Analysis of the cloned yA-sgRNA sequence within the pFC330 vector</p> Signup and view all the answers