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Questions and Answers
Which experiment involves the isolation of CRISPR-Cas9 containing the yA sgRNA guide and yA gene-targeting substrate vectors?
Which experiment involves the isolation of CRISPR-Cas9 containing the yA sgRNA guide and yA gene-targeting substrate vectors?
- EXP11: Fungal DNA extractions
- EXP9: Protoplast-mediated transformation of fungi using auxotrophic markers
- EXP7: Confirmation CRISPR-Cas9 vector with yA sgRNA guide is error-free
- EXP5: USER cloning (correct)
What is the purpose of EXP10: Evaluation of CRISPR-Cas9 performance in A. nidulans?
What is the purpose of EXP10: Evaluation of CRISPR-Cas9 performance in A. nidulans?
- To isolate CRISPR-Cas9 vector with yA sgRNA guide
- To evaluate the performance of CRISPR-Cas9 in A. nidulans (correct)
- To amplify the NHEJ mutated yA sequence
- To confirm the error-free CRISPR-Cas9 vector with yA sgRNA guide
What is the focus of EXP12: Amplification of NHEJ mutated yA sequence?
What is the focus of EXP12: Amplification of NHEJ mutated yA sequence?
- Gel purification of PCRs and linearized vector
- Analysis of cloned yA-sgRNA sequence within the pFC330 vector
- Protoplast-mediated transformation of fungi using auxotrophic markers
- Amplification of NHEJ mutated yA sequence (correct)
What is the responsibility of Dr. Neil Brown?
What is the responsibility of Dr. Neil Brown?
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Which task is NOT part of EXP3: Preparation of CRISPR vector fragments for USER cloning?
Which task is NOT part of EXP3: Preparation of CRISPR vector fragments for USER cloning?
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In which experiment is the analysis of the cloned yA-sgRNA sequence within the pFC330 vector performed?
In which experiment is the analysis of the cloned yA-sgRNA sequence within the pFC330 vector performed?
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