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Basic Local Alignment Search Tool (BLAST)

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Questions and Answers

What is the primary purpose of BLAST?

Calculating statistical significance of matches

Creating new DNA sequences

Analyzing biological sequence data (correct)

Studying gene expression levels

Who introduced the Basic Local Alignment Search Tool (BLAST)?

Stephen Altschul (correct)

Francis Crick

James Watson

Rosalind Franklin

What does BLAST help identify?

Locations of specific genes in the body

Members of gene families (correct)

Types of amino acids in protein structures

Number of chromosomes in an organism

Why is BLAST considered a crucial tool in bioinformatics?

It helps infer functional and evolutionary relationships between sequences Signup and view all the answers

Which aspect has BLAST continuously updated for improvement?

Speed and accuracy Signup and view all the answers

How many types of BLAST variants exist based on the type of sequence?

Five types Signup and view all the answers

What type of comparison does BLASTN perform?

Nucleotide query sequence to a nucleotide sequence database Signup and view all the answers

Which BLAST program compares a protein query sequence to a protein sequence database?

BLASTP Signup and view all the answers

What does TBLASTN do when comparing a protein query sequence to a nucleotide sequence database?

Translates the nucleotide sequences into six reading frames Signup and view all the answers

How is the expect value (E-value) used in BLAST searches?

To limit the number of hits to significant ones Signup and view all the answers

What does an E-value of 10e-10 < E-value < 10e-50 indicate in BLAST searches?

Closely related sequences Signup and view all the answers

When does an E-value greater than 1 indicate in BLAST searches?

Unrelated proteins Signup and view all the answers