Basic Local Alignment Search Tool (BLAST)

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Questions and Answers

What is the primary purpose of BLAST?

  • Calculating statistical significance of matches
  • Creating new DNA sequences
  • Analyzing biological sequence data (correct)
  • Studying gene expression levels

Who introduced the Basic Local Alignment Search Tool (BLAST)?

  • Stephen Altschul (correct)
  • Francis Crick
  • James Watson
  • Rosalind Franklin

What does BLAST help identify?

  • Locations of specific genes in the body
  • Members of gene families (correct)
  • Types of amino acids in protein structures
  • Number of chromosomes in an organism

Why is BLAST considered a crucial tool in bioinformatics?

<p>It helps infer functional and evolutionary relationships between sequences (B)</p> Signup and view all the answers

Which aspect has BLAST continuously updated for improvement?

<p>Speed and accuracy (B)</p> Signup and view all the answers

How many types of BLAST variants exist based on the type of sequence?

<p>Five types (A)</p> Signup and view all the answers

What type of comparison does BLASTN perform?

<p>Nucleotide query sequence to a nucleotide sequence database (B)</p> Signup and view all the answers

Which BLAST program compares a protein query sequence to a protein sequence database?

<p>BLASTP (C)</p> Signup and view all the answers

What does TBLASTN do when comparing a protein query sequence to a nucleotide sequence database?

<p>Translates the nucleotide sequences into six reading frames (D)</p> Signup and view all the answers

How is the expect value (E-value) used in BLAST searches?

<p>To limit the number of hits to significant ones (D)</p> Signup and view all the answers

What does an E-value of 10e-10 < E-value < 10e-50 indicate in BLAST searches?

<p>Closely related sequences (D)</p> Signup and view all the answers

When does an E-value greater than 1 indicate in BLAST searches?

<p>Unrelated proteins (B)</p> Signup and view all the answers

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