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Questions and Answers
What range of pH is typically maintained in the plasma of healthy individuals?
What range of pH is typically maintained in the plasma of healthy individuals?
The pH range is maintained between 7.35 and 7.45.
Why are indicator dyes and test strips considered less precise?
Why are indicator dyes and test strips considered less precise?
They are only effective within a small pH range of about 1-2 pH units.
How does pH affect protein function and structure?
How does pH affect protein function and structure?
pH influences enzymatic activity and alters the structure of proteins, affecting their efficiency.
What is a buffer solution and its function?
What is a buffer solution and its function?
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In which environments can many organisms, particularly aquatic animals, survive?
In which environments can many organisms, particularly aquatic animals, survive?
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What is the main characteristic of covalent bonding?
What is the main characteristic of covalent bonding?
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How does polar covalent bonding differ from pure covalent bonding?
How does polar covalent bonding differ from pure covalent bonding?
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What defines an ionic bond?
What defines an ionic bond?
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What is the role of hydrogen bonds in molecular interactions?
What is the role of hydrogen bonds in molecular interactions?
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According to the Bronsted-Lowry theory, what is the difference between an acid and a base?
According to the Bronsted-Lowry theory, what is the difference between an acid and a base?
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Explain the relationship between pH and hydronium ion concentration.
Explain the relationship between pH and hydronium ion concentration.
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How can pH be measured, and what is an important step before using a pH meter?
How can pH be measured, and what is an important step before using a pH meter?
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What happens during the neutralization reaction between an acid and a base?
What happens during the neutralization reaction between an acid and a base?
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What is the primary fluid medium of cells, and in what concentration is it typically found?
What is the primary fluid medium of cells, and in what concentration is it typically found?
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List two types of proteins found in cells and provide one example of each.
List two types of proteins found in cells and provide one example of each.
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What role do ions play in cellular function, particularly at the cell membrane?
What role do ions play in cellular function, particularly at the cell membrane?
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What are the key types of lipids found in cell membranes, and what is their significance?
What are the key types of lipids found in cell membranes, and what is their significance?
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How much of the mass of most cells is composed of proteins, and what range is typical?
How much of the mass of most cells is composed of proteins, and what range is typical?
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What is the typical stored carbohydrate in human cells, and in what form is it stored?
What is the typical stored carbohydrate in human cells, and in what form is it stored?
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In fat cells, what percentage of cell mass can triglycerides account for, and what is their purpose?
In fat cells, what percentage of cell mass can triglycerides account for, and what is their purpose?
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Why do most human cells not store large amounts of carbohydrates?
Why do most human cells not store large amounts of carbohydrates?
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What are the primary components of the phosphate buffer system in the human body?
What are the primary components of the phosphate buffer system in the human body?
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What is the pKa of histidine when incorporated into a protein, and how does it affect its buffering capacity?
What is the pKa of histidine when incorporated into a protein, and how does it affect its buffering capacity?
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How does the concentration of bicarbonate ions compare to that of carbonic acid in maintaining blood plasma pH?
How does the concentration of bicarbonate ions compare to that of carbonic acid in maintaining blood plasma pH?
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What happens to the pH of blood plasma when you breathe too fast, and why?
What happens to the pH of blood plasma when you breathe too fast, and why?
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Describe the roles of protein buffers in intracellular and extracellular fluids.
Describe the roles of protein buffers in intracellular and extracellular fluids.
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Which buffer system serves as the main extracellular buffer in the human body?
Which buffer system serves as the main extracellular buffer in the human body?
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What is the pKa of phosphoric acid, and why is it not effective as a buffer at pH 7?
What is the pKa of phosphoric acid, and why is it not effective as a buffer at pH 7?
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Explain why maintaining low levels of carbonic acid is necessary for blood plasma buffering.
Explain why maintaining low levels of carbonic acid is necessary for blood plasma buffering.
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What is the primary function of a buffer solution when an acid is added?
What is the primary function of a buffer solution when an acid is added?
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How does the addition of a base affect the composition of a buffer solution?
How does the addition of a base affect the composition of a buffer solution?
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Explain the significance of the Henderson-Hasselbalch equation in buffer solutions.
Explain the significance of the Henderson-Hasselbalch equation in buffer solutions.
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What role does the value of Ka play in determining the pH of a buffer solution?
What role does the value of Ka play in determining the pH of a buffer solution?
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What happens to the concentrations of HA and A- at equilibrium in a buffer solution?
What happens to the concentrations of HA and A- at equilibrium in a buffer solution?
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Define buffer capacity and how it relates to buffer solutions.
Define buffer capacity and how it relates to buffer solutions.
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In a buffer system, how can you identify if it is still effective after adding acid or base?
In a buffer system, how can you identify if it is still effective after adding acid or base?
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Describe Le Chatelier’s principle in the context of a buffer solution.
Describe Le Chatelier’s principle in the context of a buffer solution.
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Study Notes
Basic Cell Chemicals
- Basic cell chemicals are presented in the context of the cell.
- Water is the principal fluid in cells (70-85%).
- Many cell chemicals are dissolved in water.
- Chemical reactions occur in dissolved chemicals and at suspended particle surfaces.
- Key ions include K+, Mg2+, PO43-, SO42-, HCO3-, Na+, Cl-, and Ca2+.
- Different concentrations are found in extracellular and intracellular fluids.
Important Molecules in a Cell
- Water is the principal fluid medium of most cells (excluding fat cells).
- Water concentration ranges from 70% to 85%.
- Many cellular chemicals are dissolved in water, while others are suspended in solid particulate form.
- Chemical reactions occur between dissolved chemicals or at surfaces, including membranes of suspended particles
Ions
- Key ions in cells include K+, Mg2+, PO43-, SO42-, HCO3-.
- Na+, Cl- and Ca2+ are present in smaller quantities within cells.
- These ions play a role in cellular reactions and cellular control mechanisms.
- Ions at cell membranes are crucial for electrochemical impulses in nerve and muscle cells.
Proteins
- Proteins are the most abundant substances after water in most cells (10-20% of cell mass).
- Two protein categories:
- Structural proteins: Intracellular filaments and extracellular proteins (collagen, elastin).
- Functional proteins: Enzymes, hormones, and antibodies.
Lipids
- Key lipids include phospholipids and cholesterol (2% total cell mass).
- Phospholipids and cholesterol form cell membranes and intracellular barriers.
- Triglycerides (neutral fats) are present in high quantities in fat cells (95% of cell mass).
- Triglycerides are the body's main energy storage.
Carbohydrates
- Most human cells have minimal carbohydrate stores (1–3%, occasionally 6% in muscle and liver cells).
- Glucose is dissolved in extracellular fluid.
- Glycogen (an insoluble glucose polymer) is stored in small amounts.
Types of Bonding
- Chemical bonding types depend on valence electron sharing.
- Covalent bonding: Valence electrons shared as pairs between bonded atoms.
- Pure covalent bonding: Occurs with identical nonmetal atoms.
- Polar covalent bonding: Occurs with different nonmetal atoms or with a nonmetal and a metal ( a mixture of covalent and ionic).
- Ionic bonding: Valence electrons are completely transferred from one atom to another between metal and nonmetals (significant difference in electronegativity).
Polar Covalent Bonding
- Polar molecules have an overall dipole (represented by arrow to more electronegative end).
- Dipole moment is the quantitative measure of molecular polarity.
Ionic Bonding
- Valence electrons are completely transferred from one atom to another.
- Forms ionic bonds between metals and nonmetals when there's a large difference in electronegativity.
Hydrogen Bonds
- Attractive forces occur between hydrogen atoms attached to an electronegative atom of one molecule and an electronegative atom of a different molecule.
- Oxygen, nitrogen, or fluorine (with partial negative charge) are generally involved.
Acids and Bases - Bronsted-Lowry Theory
- Acid: Proton (hydrogen ion) donor.
- Base: Proton (hydrogen ion) acceptor.
Acids and Bases - Arrhenius Theory
- Acids produce hydrogen ions in solution.
- Bases produce hydroxide ions in solution.
- Neutralization occurs when hydrogen and hydroxide ions react to produce water.
pH
- pH: Expressed as the negative log of hydrogen ion (really hydronium ion) concentration ([H+]).
- Pure water: pH = 7.
- Acids: Have high [H+] and low pH.
- Bases: Have low [H+] and high pH.
Ways to Measure pH
- pH meter: Electrode measures [H+]; requires standardization.
- Indicator dyes and test strips: Less precise, suitable for field usage, small volumes, or noxious samples.
pH in Biology
- pH affects substance solubility and protein structure/function.
- Many cells/organisms thrive only within a specific pH range.
- Blood pH (7.35-7.45): Maintained by buffers
Buffer Solution
- Buffer solutions maintain relatively constant pH when strong acids/bases are added.
- Composed of a weak acid and its conjugate base (e.g., acetic acid & sodium acetate; ammonium chloride & ammonia).
pH of a Buffer Solution
- pH of a buffer solution depends on the pKₐ of the weak acid.
- Henderson-Hasselbalch Equation for buffer solution:pH = pKₐ + log([A⁻]/[HA]).
Buffering Action (Le Chatelier's Principle)
- Equilibrium in buffer systems: HA ⇌ H⁺ + A⁻
- Adding acid shifts equilibrium to the left (more HA, less A⁻), but [H⁺] remains relatively constant.
- Adding base shifts equilibrium to the right (more A⁻, less HA) but [H⁺] also remains relatively constant.
Important Buffers in the Human Body
- Proteins, phosphates, and carbonic acid are important buffers.
- Protein Buffer system: Proteins are enormous molecules constructed from smaller compounds called amino acids containing Histidine
- Histidine is a weak base, easily converted to its conjugate acid whose pKₐ value can be as high as 7.0 in proteins.
- Phosphate buffer system: Phosphoric acid's first pKₐ value is too low for effective buffering around pH 7, effectively functioning around pH 7.2 using dihydrogen phosphate and hydrogen phosphate ions
- Carbonic acid buffer system: Most important extracellular buffer; H₂CO₃ and bicarbonate ions maintain plasma pH of 7.4; H₂CO₃ has a pKₐ of 6.1. at body temperature, requiring a higher HCO₃⁻ concentration to reach physiological pH (20 times more).
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Description
Explore the fundamental chemicals and ions essential for cellular function. This quiz covers the role of water as a cell medium, the importance of various ions, and how chemical reactions occur in cellular environments. Test your knowledge on key components that sustain life at the cellular level.