Basic Cell Chemicals and Molecules
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Questions and Answers

What range of pH is typically maintained in the plasma of healthy individuals?

The pH range is maintained between 7.35 and 7.45.

Why are indicator dyes and test strips considered less precise?

They are only effective within a small pH range of about 1-2 pH units.

How does pH affect protein function and structure?

pH influences enzymatic activity and alters the structure of proteins, affecting their efficiency.

What is a buffer solution and its function?

<p>A buffer solution is a mixture of a weak acid and its conjugate base that maintains a relatively constant pH.</p> Signup and view all the answers

In which environments can many organisms, particularly aquatic animals, survive?

<p>Many organisms can only survive in specific pH environments tailored to their biological needs.</p> Signup and view all the answers

What is the main characteristic of covalent bonding?

<p>In covalent bonding, valence electrons are shared as pairs between bonded atoms.</p> Signup and view all the answers

How does polar covalent bonding differ from pure covalent bonding?

<p>Polar covalent bonding involves atoms of different electronegativities, resulting in a partial charge, while pure covalent bonding occurs between identical nonmetals without charge separation.</p> Signup and view all the answers

What defines an ionic bond?

<p>An ionic bond is formed when valence electrons are completely transferred from one atom to another, typically between metals and nonmetals with significant electronegativity differences.</p> Signup and view all the answers

What is the role of hydrogen bonds in molecular interactions?

<p>Hydrogen bonds are attractive forces between a hydrogen atom bonded to an electronegative atom and another electronegative atom in a different molecule.</p> Signup and view all the answers

According to the Bronsted-Lowry theory, what is the difference between an acid and a base?

<p>An acid is defined as a proton (H+) donor, while a base is a proton (H+) acceptor.</p> Signup and view all the answers

Explain the relationship between pH and hydronium ion concentration.

<p>pH is expressed as the negative logarithm of hydronium ion concentration, meaning lower pH values correspond to higher [H+] concentrations.</p> Signup and view all the answers

How can pH be measured, and what is an important step before using a pH meter?

<p>pH can be measured using a pH meter, and it is crucial to standardize (calibrate) the meter before use to ensure accuracy.</p> Signup and view all the answers

What happens during the neutralization reaction between an acid and a base?

<p>During neutralization, hydrogen ions (H+) from the acid react with hydroxide ions (OH-) from the base to produce water.</p> Signup and view all the answers

What is the primary fluid medium of cells, and in what concentration is it typically found?

<p>The primary fluid medium of cells is water, which is typically found in a concentration of 70% to 85%.</p> Signup and view all the answers

List two types of proteins found in cells and provide one example of each.

<p>The two types of proteins are structural proteins, exemplified by intracellular filaments, and functional proteins, such as enzymes.</p> Signup and view all the answers

What role do ions play in cellular function, particularly at the cell membrane?

<p>Ions provide inorganic chemicals for cellular reactions and are essential for transmitting electrochemical impulses in nerve and muscle fibers.</p> Signup and view all the answers

What are the key types of lipids found in cell membranes, and what is their significance?

<p>Phospholipids and cholesterol are key types of lipids found in cell membranes, providing structure and forming barriers between cell compartments.</p> Signup and view all the answers

How much of the mass of most cells is composed of proteins, and what range is typical?

<p>Proteins constitute about 10% to 20% of the mass of most cells.</p> Signup and view all the answers

What is the typical stored carbohydrate in human cells, and in what form is it stored?

<p>The typical stored carbohydrate in human cells is glycogen, an insoluble polymer of glucose.</p> Signup and view all the answers

In fat cells, what percentage of cell mass can triglycerides account for, and what is their purpose?

<p>In fat cells, triglycerides can account for as much as 95% of the cell mass, serving as the body’s main storehouse for energy.</p> Signup and view all the answers

Why do most human cells not store large amounts of carbohydrates?

<p>Most human cells do not store large amounts of carbohydrates because they primarily rely on glucose present in the extracellular fluid for energy.</p> Signup and view all the answers

What are the primary components of the phosphate buffer system in the human body?

<p>The primary components are H2PO4- and HPO42-.</p> Signup and view all the answers

What is the pKa of histidine when incorporated into a protein, and how does it affect its buffering capacity?

<p>The pKa of histidine when incorporated into a protein can be as high as 7.0, enabling effective buffering around pH 7.</p> Signup and view all the answers

How does the concentration of bicarbonate ions compare to that of carbonic acid in maintaining blood plasma pH?

<p>The concentration of bicarbonate ions (HCO3-) must be 20 times larger than that of carbonic acid (H2CO3).</p> Signup and view all the answers

What happens to the pH of blood plasma when you breathe too fast, and why?

<p>The pH of blood plasma increases because rapid breathing leads to excessive loss of carbon dioxide, reducing carbonic acid concentration.</p> Signup and view all the answers

Describe the roles of protein buffers in intracellular and extracellular fluids.

<p>Protein buffers help maintain a constant pH inside cells and also play a role in buffering in extracellular fluids.</p> Signup and view all the answers

Which buffer system serves as the main extracellular buffer in the human body?

<p>The carbonic acid buffer system serves as the main extracellular buffer.</p> Signup and view all the answers

What is the pKa of phosphoric acid, and why is it not effective as a buffer at pH 7?

<p>The pKa of phosphoric acid is 2.1, which is too low for effective buffering at pH 7.</p> Signup and view all the answers

Explain why maintaining low levels of carbonic acid is necessary for blood plasma buffering.

<p>Maintaining low levels of carbonic acid prevents disruption of osmotic balance between plasma and intracellular fluids.</p> Signup and view all the answers

What is the primary function of a buffer solution when an acid is added?

<p>The buffer solution will shift the equilibrium to increase the concentration of HA and decrease the concentration of A-.</p> Signup and view all the answers

How does the addition of a base affect the composition of a buffer solution?

<p>The addition of a base will consume HA, leading to a decrease in HA and an increase in A-.</p> Signup and view all the answers

Explain the significance of the Henderson-Hasselbalch equation in buffer solutions.

<p>The Henderson-Hasselbalch equation relates the pH of a buffer solution to the pKa and the ratio of the concentrations of the base and acid.</p> Signup and view all the answers

What role does the value of Ka play in determining the pH of a buffer solution?

<p>Ka indicates the strength of the acid; a smaller Ka value suggests a weaker acid, thus influencing the concentrations and pH of the buffer system.</p> Signup and view all the answers

What happens to the concentrations of HA and A- at equilibrium in a buffer solution?

<p>At equilibrium, the product concentrations [H+] and [A-] are established such that the equilibrium constant Ka holds true.</p> Signup and view all the answers

Define buffer capacity and how it relates to buffer solutions.

<p>Buffer capacity is the ability of a buffer solution to resist pH changes upon the addition of acids or bases.</p> Signup and view all the answers

In a buffer system, how can you identify if it is still effective after adding acid or base?

<p>A buffer remains effective as long as sufficient concentrations of both HA and A- are present in the solution.</p> Signup and view all the answers

Describe Le Chatelier’s principle in the context of a buffer solution.

<p>Le Chatelier's principle states that if a change is imposed on a system at equilibrium, the system will adjust to counteract that change.</p> Signup and view all the answers

Study Notes

Basic Cell Chemicals

  • Basic cell chemicals are presented in the context of the cell.
  • Water is the principal fluid in cells (70-85%).
  • Many cell chemicals are dissolved in water.
  • Chemical reactions occur in dissolved chemicals and at suspended particle surfaces.
  • Key ions include K+, Mg2+, PO43-, SO42-, HCO3-, Na+, Cl-, and Ca2+.
  • Different concentrations are found in extracellular and intracellular fluids.

Important Molecules in a Cell

  • Water is the principal fluid medium of most cells (excluding fat cells).
  • Water concentration ranges from 70% to 85%.
  • Many cellular chemicals are dissolved in water, while others are suspended in solid particulate form.
  • Chemical reactions occur between dissolved chemicals or at surfaces, including membranes of suspended particles

Ions

  • Key ions in cells include K+, Mg2+, PO43-, SO42-, HCO3-.
  • Na+, Cl- and Ca2+ are present in smaller quantities within cells.
  • These ions play a role in cellular reactions and cellular control mechanisms.
  • Ions at cell membranes are crucial for electrochemical impulses in nerve and muscle cells.

Proteins

  • Proteins are the most abundant substances after water in most cells (10-20% of cell mass).
  • Two protein categories:
    • Structural proteins: Intracellular filaments and extracellular proteins (collagen, elastin).
    • Functional proteins: Enzymes, hormones, and antibodies.

Lipids

  • Key lipids include phospholipids and cholesterol (2% total cell mass).
  • Phospholipids and cholesterol form cell membranes and intracellular barriers.
  • Triglycerides (neutral fats) are present in high quantities in fat cells (95% of cell mass).
  • Triglycerides are the body's main energy storage.

Carbohydrates

  • Most human cells have minimal carbohydrate stores (1–3%, occasionally 6% in muscle and liver cells).
  • Glucose is dissolved in extracellular fluid.
  • Glycogen (an insoluble glucose polymer) is stored in small amounts.

Types of Bonding

  • Chemical bonding types depend on valence electron sharing.
    • Covalent bonding: Valence electrons shared as pairs between bonded atoms.
    • Pure covalent bonding: Occurs with identical nonmetal atoms.
    • Polar covalent bonding: Occurs with different nonmetal atoms or with a nonmetal and a metal ( a mixture of covalent and ionic).
    • Ionic bonding: Valence electrons are completely transferred from one atom to another between metal and nonmetals (significant difference in electronegativity).

Polar Covalent Bonding

  • Polar molecules have an overall dipole (represented by arrow to more electronegative end).
  • Dipole moment is the quantitative measure of molecular polarity.

Ionic Bonding

  • Valence electrons are completely transferred from one atom to another.
  • Forms ionic bonds between metals and nonmetals when there's a large difference in electronegativity.

Hydrogen Bonds

  • Attractive forces occur between hydrogen atoms attached to an electronegative atom of one molecule and an electronegative atom of a different molecule.
  • Oxygen, nitrogen, or fluorine (with partial negative charge) are generally involved.

Acids and Bases - Bronsted-Lowry Theory

  • Acid: Proton (hydrogen ion) donor.
  • Base: Proton (hydrogen ion) acceptor.

Acids and Bases - Arrhenius Theory

  • Acids produce hydrogen ions in solution.
  • Bases produce hydroxide ions in solution.
  • Neutralization occurs when hydrogen and hydroxide ions react to produce water.

pH

  • pH: Expressed as the negative log of hydrogen ion (really hydronium ion) concentration ([H+]).
  • Pure water: pH = 7.
  • Acids: Have high [H+] and low pH.
  • Bases: Have low [H+] and high pH.

Ways to Measure pH

  • pH meter: Electrode measures [H+]; requires standardization.
  • Indicator dyes and test strips: Less precise, suitable for field usage, small volumes, or noxious samples.

pH in Biology

  • pH affects substance solubility and protein structure/function.
  • Many cells/organisms thrive only within a specific pH range.
  • Blood pH (7.35-7.45): Maintained by buffers

Buffer Solution

  • Buffer solutions maintain relatively constant pH when strong acids/bases are added.
  • Composed of a weak acid and its conjugate base (e.g., acetic acid & sodium acetate; ammonium chloride & ammonia).

pH of a Buffer Solution

  • pH of a buffer solution depends on the pKₐ of the weak acid.
  • Henderson-Hasselbalch Equation for buffer solution:pH = pKₐ + log([A⁻]/[HA]).

Buffering Action (Le Chatelier's Principle)

  • Equilibrium in buffer systems: HA ⇌ H⁺ + A⁻
  • Adding acid shifts equilibrium to the left (more HA, less A⁻), but [H⁺] remains relatively constant.
  • Adding base shifts equilibrium to the right (more A⁻, less HA) but [H⁺] also remains relatively constant.

Important Buffers in the Human Body

  • Proteins, phosphates, and carbonic acid are important buffers.
  • Protein Buffer system: Proteins are enormous molecules constructed from smaller compounds called amino acids containing Histidine
    • Histidine is a weak base, easily converted to its conjugate acid whose pKₐ value can be as high as 7.0 in proteins.
  • Phosphate buffer system: Phosphoric acid's first pKₐ value is too low for effective buffering around pH 7, effectively functioning around pH 7.2 using dihydrogen phosphate and hydrogen phosphate ions
  • Carbonic acid buffer system: Most important extracellular buffer; H₂CO₃ and bicarbonate ions maintain plasma pH of 7.4; H₂CO₃ has a pKₐ of 6.1. at body temperature, requiring a higher HCO₃⁻ concentration to reach physiological pH (20 times more).

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Basic Cell Chemicals PDF

Description

Explore the fundamental chemicals and ions essential for cellular function. This quiz covers the role of water as a cell medium, the importance of various ions, and how chemical reactions occur in cellular environments. Test your knowledge on key components that sustain life at the cellular level.

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