Bacteriophage Therapy and Antibiotic Resistance

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Questions and Answers

What is the most likely reason the shigella infection persists despite the use of azithromycin?

  • The patient did not take the medication as prescribed.
  • The shigella strain is resistant to azithromycin. (correct)
  • The patient's immune system is compromised.
  • Azithromycin is not an effective treatment for shigella infections.

What does the passage suggest is the doctor's primary concern when prescribing ciprofloxacin?

  • The potential for allergic reactions.
  • The possibility of drug interactions.
  • The risk of developing antibiotic resistance.
  • The potential for damage to the patient's microbiome. (correct)

According to the passage, what does the term "antibiotic of last resort" refer to?

  • An antibiotic that is only effective against a specific type of bacteria.
  • An antibiotic that is more effective than other antibiotics.
  • An antibiotic that is typically used as a first-line treatment.
  • An antibiotic that is only used when other antibiotics have failed. (correct)

What is the main idea of the passage?

<p>To illustrate the dangers of antibiotic resistance and the need for alternative treatments. (D)</p> Signup and view all the answers

What is the likely implication of "four people die every hour from an antibiotic-resistant bacterial infection in the United States"?

<p>Antibiotic resistance is a growing threat to public health. (B)</p> Signup and view all the answers

How does the shigella bacteria evade the effects of azithromycin?

<p>It produces enzymes that neutralize the antibiotic molecules. (A)</p> Signup and view all the answers

Which of these is NOT a symptom mentioned in the passage for the patient's shigella infection?

<p>Nausea (D)</p> Signup and view all the answers

What is the implication of the passage's focus on "bacteriophage therapy"?

<p>Bacteriophage therapy is considered a promising alternative to antibiotics. (A)</p> Signup and view all the answers

Which of the following statements accurately describes the impact of the Iron Curtain on the development of phage therapy?

<p>Soviet researchers' work on phage therapy remained largely unknown to the rest of the world, hindering its global development. (A)</p> Signup and view all the answers

What prompted the renewed interest in phage therapy in recent years?

<p>The increasing number of antibiotic-resistant bacteria, posing a significant health threat. (A)</p> Signup and view all the answers

What makes phages a potentially valuable tool in combating bacterial infections?

<p>They target and destroy specific types of bacteria without harming beneficial bacteria. (B)</p> Signup and view all the answers

Which of the following institutions is mentioned in the text as a pioneer in phage therapy?

<p>The Hirszfeld Institute (A), The Eliava Institute (C)</p> Signup and view all the answers

What is the term used to describe the collection of viruses that naturally live in and on humans?

<p>Human virome (B)</p> Signup and view all the answers

Why are phages sometimes called "living medicines"?

<p>They are derived from living organisms and have biological activity. (C)</p> Signup and view all the answers

What is the significance of the fact that phage medicines were sold in pharmacies in Tbilisi, Georgia?

<p>It demonstrates that phage therapy is a well-established and widely accepted practice in some parts of the world. (B), It indicates that phage therapy has been used to treat a wide range of infections for a long time. (D)</p> Signup and view all the answers

Which of the following is NOT mentioned as a potential benefit of phage therapy?

<p>Prevention of antibiotic resistance development. (D)</p> Signup and view all the answers

Which of the following is a valid reason why phage therapy is considered a promising alternative to antibiotics?

<p>Phages are effective in treating infections that respond poorly to antibiotics. (B), Phages can target a wide range of bacteria, including antibiotic-resistant strains. (C)</p> Signup and view all the answers

What was the primary purpose of the remedies made at Tbilisi's Institute of Bacteriophage, Microbiology, and Virology?

<p>To treat sore throats (B)</p> Signup and view all the answers

Who was the founder of the institute that developed phage remedies?

<p>Giorgi Eliava (A)</p> Signup and view all the answers

How were phages matched to patients at the institute?

<p>Based on bacterial cultures from samples (B)</p> Signup and view all the answers

What distinguishes phages from antibiotics according to the content provided?

<p>Phages are personalized therapies and living medicines (D)</p> Signup and view all the answers

What type of infections did patients at the institute commonly battle?

<p>Stubborn skin and gut infections (D)</p> Signup and view all the answers

Why were phages viewed with skepticism in the American medical establishment?

<p>They were foreign and less reliable compared to antibiotics (B)</p> Signup and view all the answers

What marked a significant shift in the FDA's view of phage therapy?

<p>The approval of the first phage therapy trial in history (A)</p> Signup and view all the answers

What process do antibiotics primarily use to eliminate bacteria?

<p>Preventing the replication of bacterial cells (B)</p> Signup and view all the answers

What is the composition of most phages that target human bacterial pathogens?

<p>An icosahedral head with a protein tail (B)</p> Signup and view all the answers

What occurs immediately after a lytic phage injects its genome into a bacterium?

<p>The bacterial DNA and protein synthesis machinery are hijacked (D)</p> Signup and view all the answers

What therapeutic potential was first described by Felix d'Hérelle in 1919?

<p>The application of phage therapy to treat dysentery (B)</p> Signup and view all the answers

What ultimately led to the decline in interest in phage therapy among Western scientists?

<p>The widespread success of penicillin during and after World War II (A)</p> Signup and view all the answers

What characterizes strictly lytic phages in their interaction with host bacteria?

<p>They cause the host cell to reproduce phage and then kill it. (C)</p> Signup and view all the answers

Who founded the Eliava Institute devoted to phage therapy research?

<p>Giorgi Eliava (C)</p> Signup and view all the answers

What is one of the main reasons phages are considered safe for mammalian cells?

<p>They have evolved to only infect certain target bacteria. (C)</p> Signup and view all the answers

How do phages reproduce after infecting a bacterial cell?

<p>By using the bacterial machinery to produce copies (D)</p> Signup and view all the answers

What is the primary function of the robot Neptune in the lab?

<p>To automate the testing of phages against bacteria (B)</p> Signup and view all the answers

What does Neptune primarily measure every half hour during its operation?

<p>The population of bacterial cells in the solution (B)</p> Signup and view all the answers

What kind of environment does Neptune create for the bacteria it tests?

<p>A nutrient-rich soup with glucose and amino acids (D)</p> Signup and view all the answers

Why has phage therapy research gained support from institutions like the National Institute of Allergy and Infectious Diseases?

<p>Due to a rise in antibiotic-resistant bacterial strains (C)</p> Signup and view all the answers

What is the significance of Neptune being able to extrapolate information for novel strains and phages?

<p>It aids in predicting phage effectiveness against emerging bacterial threats. (A)</p> Signup and view all the answers

How does Neptune create conditions for testing effectiveness against shigella?

<p>By preloading with a specific nutrient solution found in the gut (C)</p> Signup and view all the answers

What type of laboratory equipment does Neptune utilize to perform its tests?

<p>A series of shallow tubes known as 96-well plates (D)</p> Signup and view all the answers

What does Neptune do after loading the 96-well plates with bacteria and phages?

<p>It places the plates into an incubator to monitor phage activity (A)</p> Signup and view all the answers

How does shigella manage to evade the effects of colistin?

<p>By changing its outer cell membrane structure (C)</p> Signup and view all the answers

What do bacteriophages specifically target?

<p>Bacterial infections (B)</p> Signup and view all the answers

What is the significance of describing shigella as a 'superbug'?

<p>It signifies its ability to resist multiple antibiotics (C)</p> Signup and view all the answers

Why are bacteriophages considered a potential solution to antibiotic resistance?

<p>They can specifically kill bacteria without affecting human cells (B)</p> Signup and view all the answers

What is a significant consequence of the failure to control antibiotic-resistant infections?

<p>Increased rate of complications and death from infections (D)</p> Signup and view all the answers

Where are bacteriophages commonly found?

<p>In locations where bacteria are prevalent (B)</p> Signup and view all the answers

What does the phrase 'post-antibiotic era' imply?

<p>Traditional antibiotics are becoming ineffective (D)</p> Signup and view all the answers

How do bacteria typically respond to an antibiotic like colistin initially?

<p>Sensitive bacteria are killed, but resistant ones thrive later (B)</p> Signup and view all the answers

Flashcards

Phage Therapy

A treatment using bacteriophages to combat bacterial infections.

Bacteriophages

Viruses that specifically infect and destroy bacteria without harming human cells.

Iron Curtain

A metaphorical barrier that limited knowledge exchange during the Cold War, especially for Soviet research.

Superbugs

Bacteria that have become resistant to antibiotics, posing significant treatment challenges.

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Eliava Institute

A prominent center in Georgia known for pioneering phage therapy research and application.

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Human Virome

The collection of viruses present in and on the human body, including beneficial phages.

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Microbiome

The community of microbes that live in and on our bodies, crucial for health.

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Living Medicines

A term used to describe phages as natural antiviral agents in health treatment.

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Kidney failure

A medical condition where the kidneys lose their ability to function properly.

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Colistin

An antibiotic used to treat infections caused by certain bacteria.

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Antibiotic resistance

When bacteria evolve to resist the effects of antibiotics.

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Ubiquitous

Present everywhere; especially in relation to phages in the environment.

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Trojan horse (in context)

A strategy used by bacteriophages to infiltrate bacteria.

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Post-antibiotic era

A period where common infections can no longer be treated effectively with antibiotics.

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Giorgi Eliava

A Soviet scientist who founded the Eliava Institute known for phage research.

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Personalized medicine

Tailoring medical treatment to the individual characteristics of each patient.

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FDA approval

The process for the U.S. Food and Drug Administration to authorize a treatment for public use.

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Stubborn infections

Infections that do not respond quickly to standard treatments.

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Comparison to antibiotics

Phages are living medicines, unlike static antibiotics that kill bacteria indiscriminately.

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Bacteriophage Therapy

A treatment that uses viruses to target and destroy bacteria.

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Shigella

A genus of bacteria that can cause gastrointestinal infections, leading to bloody diarrhea.

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Azithromycin

An antibiotic commonly used to treat bacterial infections like stomach bugs.

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Ciprofloxacin

A broad-spectrum antibiotic used for more severe infections when first-line treatments fail.

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Gastrointestinal Infection

An infection affecting the stomach and intestines, often causing diarrhea and vomiting.

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Neptune

A biotech robot used for microbial warfare testing.

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96-well plate

A set of shallow tubes used to grow bacteria and phages.

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Microbial warfare

The process of testing interactions between bacteria and phages.

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Artificial intelligence in phage research

AI aids in predicting effective phage combinations.

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Clinical trials for phage therapy

Tests to evaluate the effectiveness of phages against bacteria.

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Icosahedral head

The geometric structure of certain phages with 20 flat faces and protein composition.

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Lytic phage

A type of bacteriophage that causes the host bacterium to lyse or burst after replication.

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Phage genome injection

The process where a phage injects its genetic material into a bacterium's periplasm.

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Bacterial hijacking

When a phage takes control of bacterial machinery to replicate itself.

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Phage progeny

The offspring of a phage, often numbering in the hundreds, that emerge after lysis.

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Frederick Twort

An English bacteriologist known for early discoveries of antibacterial viral agents.

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Felix d'Hérelle

A microbiologist credited with identifying the therapeutic potential of phages in 1919.

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Study Notes

Bacteriophage Therapy

  • Bacteriophages (phages) are viruses that infect and kill bacteria.
  • Phages are abundant in water, soil, and sewage.
  • Phages have been used to treat bacterial infections for over 100 years, but interest waned with the development of antibiotics.
  • Phages are specific to target bacteria, unlike antibiotics that are broad-spectrum.
  • Phage therapy is showing renewed interest due to rising antibiotic resistance.
  • Phages inject their DNA into bacteria, hijacking the bacterial machinery to create more phages, eventually killing the bacteria.
  • Phages have specialized "tails" for injecting genetic material into bacteria.

Antibiotic Resistance

  • Antibiotic resistance is a major problem globally.
  • Four people die from antibiotic-resistant bacterial infections every hour in the U.S.
  • Bacteria can develop resistance to antibiotics rapidly through genetic mutations or via enzymes that destroy the antibiotics.

Shigella Infections

  • Shigella is a bacteria that causes severe diarrheal illness.
  • Some strains of shigella are resistant to multiple antibiotics.
  • Symptoms can include severe diarrhea that can progress to bloody diarrhea.
  • Antibiotic treatment may not be effective in resistant cases.

Phage Therapy Process

  • Researchers use sophisticated robotics (e.g., Neptune) to identify suitable phages for specific bacteria strains.
  • The process involves identifying the bacteria that causes the infection.
  • The researchers then match the appropriate phages to the bacteria, growing them in a medium with the target bacteria.
  • The optimal phage strains are tested with the target bacteria in various concentrations to assess their effectiveness and efficiency.
  • Phages are harvested from environments like sewage, which are rich in bacteria.

Advantages of Phage Therapy

  • Phage therapy targets only the harmful bacteria, leaving beneficial gut bacteria unaffected.
  • Phages replicate rapidly, outcompeting the bacteria.
  • Phages are more specific to bacteria, thus potentially causing fewer side effects.

Phage Therapy Research and Development

  • There are at least 50 phage clinical trials in progress.
  • Phage startups are emerging.
  • Organizations like the National Institute of Allergy and Infectious Diseases are supporting research in phage therapy.

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