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Questions and Answers
What is the most likely reason the shigella infection persists despite the use of azithromycin?
What is the most likely reason the shigella infection persists despite the use of azithromycin?
What does the passage suggest is the doctor's primary concern when prescribing ciprofloxacin?
What does the passage suggest is the doctor's primary concern when prescribing ciprofloxacin?
According to the passage, what does the term "antibiotic of last resort" refer to?
According to the passage, what does the term "antibiotic of last resort" refer to?
What is the main idea of the passage?
What is the main idea of the passage?
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What is the likely implication of "four people die every hour from an antibiotic-resistant bacterial infection in the United States"?
What is the likely implication of "four people die every hour from an antibiotic-resistant bacterial infection in the United States"?
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How does the shigella bacteria evade the effects of azithromycin?
How does the shigella bacteria evade the effects of azithromycin?
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Which of these is NOT a symptom mentioned in the passage for the patient's shigella infection?
Which of these is NOT a symptom mentioned in the passage for the patient's shigella infection?
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What is the implication of the passage's focus on "bacteriophage therapy"?
What is the implication of the passage's focus on "bacteriophage therapy"?
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Which of the following statements accurately describes the impact of the Iron Curtain on the development of phage therapy?
Which of the following statements accurately describes the impact of the Iron Curtain on the development of phage therapy?
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What prompted the renewed interest in phage therapy in recent years?
What prompted the renewed interest in phage therapy in recent years?
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What makes phages a potentially valuable tool in combating bacterial infections?
What makes phages a potentially valuable tool in combating bacterial infections?
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Which of the following institutions is mentioned in the text as a pioneer in phage therapy?
Which of the following institutions is mentioned in the text as a pioneer in phage therapy?
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What is the term used to describe the collection of viruses that naturally live in and on humans?
What is the term used to describe the collection of viruses that naturally live in and on humans?
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Why are phages sometimes called "living medicines"?
Why are phages sometimes called "living medicines"?
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What is the significance of the fact that phage medicines were sold in pharmacies in Tbilisi, Georgia?
What is the significance of the fact that phage medicines were sold in pharmacies in Tbilisi, Georgia?
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Which of the following is NOT mentioned as a potential benefit of phage therapy?
Which of the following is NOT mentioned as a potential benefit of phage therapy?
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Which of the following is a valid reason why phage therapy is considered a promising alternative to antibiotics?
Which of the following is a valid reason why phage therapy is considered a promising alternative to antibiotics?
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What was the primary purpose of the remedies made at Tbilisi's Institute of Bacteriophage, Microbiology, and Virology?
What was the primary purpose of the remedies made at Tbilisi's Institute of Bacteriophage, Microbiology, and Virology?
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Who was the founder of the institute that developed phage remedies?
Who was the founder of the institute that developed phage remedies?
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How were phages matched to patients at the institute?
How were phages matched to patients at the institute?
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What distinguishes phages from antibiotics according to the content provided?
What distinguishes phages from antibiotics according to the content provided?
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What type of infections did patients at the institute commonly battle?
What type of infections did patients at the institute commonly battle?
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Why were phages viewed with skepticism in the American medical establishment?
Why were phages viewed with skepticism in the American medical establishment?
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What marked a significant shift in the FDA's view of phage therapy?
What marked a significant shift in the FDA's view of phage therapy?
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What process do antibiotics primarily use to eliminate bacteria?
What process do antibiotics primarily use to eliminate bacteria?
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What is the composition of most phages that target human bacterial pathogens?
What is the composition of most phages that target human bacterial pathogens?
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What occurs immediately after a lytic phage injects its genome into a bacterium?
What occurs immediately after a lytic phage injects its genome into a bacterium?
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What therapeutic potential was first described by Felix d'Hérelle in 1919?
What therapeutic potential was first described by Felix d'Hérelle in 1919?
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What ultimately led to the decline in interest in phage therapy among Western scientists?
What ultimately led to the decline in interest in phage therapy among Western scientists?
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What characterizes strictly lytic phages in their interaction with host bacteria?
What characterizes strictly lytic phages in their interaction with host bacteria?
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Who founded the Eliava Institute devoted to phage therapy research?
Who founded the Eliava Institute devoted to phage therapy research?
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What is one of the main reasons phages are considered safe for mammalian cells?
What is one of the main reasons phages are considered safe for mammalian cells?
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How do phages reproduce after infecting a bacterial cell?
How do phages reproduce after infecting a bacterial cell?
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What is the primary function of the robot Neptune in the lab?
What is the primary function of the robot Neptune in the lab?
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What does Neptune primarily measure every half hour during its operation?
What does Neptune primarily measure every half hour during its operation?
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What kind of environment does Neptune create for the bacteria it tests?
What kind of environment does Neptune create for the bacteria it tests?
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Why has phage therapy research gained support from institutions like the National Institute of Allergy and Infectious Diseases?
Why has phage therapy research gained support from institutions like the National Institute of Allergy and Infectious Diseases?
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What is the significance of Neptune being able to extrapolate information for novel strains and phages?
What is the significance of Neptune being able to extrapolate information for novel strains and phages?
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How does Neptune create conditions for testing effectiveness against shigella?
How does Neptune create conditions for testing effectiveness against shigella?
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What type of laboratory equipment does Neptune utilize to perform its tests?
What type of laboratory equipment does Neptune utilize to perform its tests?
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What does Neptune do after loading the 96-well plates with bacteria and phages?
What does Neptune do after loading the 96-well plates with bacteria and phages?
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How does shigella manage to evade the effects of colistin?
How does shigella manage to evade the effects of colistin?
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What do bacteriophages specifically target?
What do bacteriophages specifically target?
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What is the significance of describing shigella as a 'superbug'?
What is the significance of describing shigella as a 'superbug'?
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Why are bacteriophages considered a potential solution to antibiotic resistance?
Why are bacteriophages considered a potential solution to antibiotic resistance?
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What is a significant consequence of the failure to control antibiotic-resistant infections?
What is a significant consequence of the failure to control antibiotic-resistant infections?
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Where are bacteriophages commonly found?
Where are bacteriophages commonly found?
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What does the phrase 'post-antibiotic era' imply?
What does the phrase 'post-antibiotic era' imply?
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How do bacteria typically respond to an antibiotic like colistin initially?
How do bacteria typically respond to an antibiotic like colistin initially?
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Flashcards
Phage Therapy
Phage Therapy
A treatment using bacteriophages to combat bacterial infections.
Bacteriophages
Bacteriophages
Viruses that specifically infect and destroy bacteria without harming human cells.
Iron Curtain
Iron Curtain
A metaphorical barrier that limited knowledge exchange during the Cold War, especially for Soviet research.
Superbugs
Superbugs
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Eliava Institute
Eliava Institute
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Human Virome
Human Virome
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Microbiome
Microbiome
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Living Medicines
Living Medicines
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Kidney failure
Kidney failure
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Colistin
Colistin
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Antibiotic resistance
Antibiotic resistance
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Ubiquitous
Ubiquitous
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Trojan horse (in context)
Trojan horse (in context)
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Post-antibiotic era
Post-antibiotic era
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Giorgi Eliava
Giorgi Eliava
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Personalized medicine
Personalized medicine
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FDA approval
FDA approval
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Stubborn infections
Stubborn infections
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Comparison to antibiotics
Comparison to antibiotics
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Bacteriophage Therapy
Bacteriophage Therapy
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Shigella
Shigella
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Azithromycin
Azithromycin
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Ciprofloxacin
Ciprofloxacin
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Gastrointestinal Infection
Gastrointestinal Infection
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Neptune
Neptune
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96-well plate
96-well plate
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Microbial warfare
Microbial warfare
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Artificial intelligence in phage research
Artificial intelligence in phage research
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Clinical trials for phage therapy
Clinical trials for phage therapy
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Icosahedral head
Icosahedral head
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Lytic phage
Lytic phage
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Phage genome injection
Phage genome injection
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Bacterial hijacking
Bacterial hijacking
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Phage progeny
Phage progeny
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Frederick Twort
Frederick Twort
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Felix d'Hérelle
Felix d'Hérelle
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Study Notes
Bacteriophage Therapy
- Bacteriophages (phages) are viruses that infect and kill bacteria.
- Phages are abundant in water, soil, and sewage.
- Phages have been used to treat bacterial infections for over 100 years, but interest waned with the development of antibiotics.
- Phages are specific to target bacteria, unlike antibiotics that are broad-spectrum.
- Phage therapy is showing renewed interest due to rising antibiotic resistance.
- Phages inject their DNA into bacteria, hijacking the bacterial machinery to create more phages, eventually killing the bacteria.
- Phages have specialized "tails" for injecting genetic material into bacteria.
Antibiotic Resistance
- Antibiotic resistance is a major problem globally.
- Four people die from antibiotic-resistant bacterial infections every hour in the U.S.
- Bacteria can develop resistance to antibiotics rapidly through genetic mutations or via enzymes that destroy the antibiotics.
Shigella Infections
- Shigella is a bacteria that causes severe diarrheal illness.
- Some strains of shigella are resistant to multiple antibiotics.
- Symptoms can include severe diarrhea that can progress to bloody diarrhea.
- Antibiotic treatment may not be effective in resistant cases.
Phage Therapy Process
- Researchers use sophisticated robotics (e.g., Neptune) to identify suitable phages for specific bacteria strains.
- The process involves identifying the bacteria that causes the infection.
- The researchers then match the appropriate phages to the bacteria, growing them in a medium with the target bacteria.
- The optimal phage strains are tested with the target bacteria in various concentrations to assess their effectiveness and efficiency.
- Phages are harvested from environments like sewage, which are rich in bacteria.
Advantages of Phage Therapy
- Phage therapy targets only the harmful bacteria, leaving beneficial gut bacteria unaffected.
- Phages replicate rapidly, outcompeting the bacteria.
- Phages are more specific to bacteria, thus potentially causing fewer side effects.
Phage Therapy Research and Development
- There are at least 50 phage clinical trials in progress.
- Phage startups are emerging.
- Organizations like the National Institute of Allergy and Infectious Diseases are supporting research in phage therapy.
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Description
Explore the world of bacteriophage therapy and its role in combating antibiotic-resistant bacteria. This quiz covers how phages work, their specificity, and the significance of addressing antibiotic resistance, particularly in infections like those caused by Shigella. Test your knowledge on these crucial topics in microbiology and infectious diseases.