Bacteriophage Therapy and Antibiotic Resistance
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Questions and Answers

What is the most likely reason the shigella infection persists despite the use of azithromycin?

  • The patient did not take the medication as prescribed.
  • The shigella strain is resistant to azithromycin. (correct)
  • The patient's immune system is compromised.
  • Azithromycin is not an effective treatment for shigella infections.
  • What does the passage suggest is the doctor's primary concern when prescribing ciprofloxacin?

  • The potential for allergic reactions.
  • The possibility of drug interactions.
  • The risk of developing antibiotic resistance.
  • The potential for damage to the patient's microbiome. (correct)
  • According to the passage, what does the term "antibiotic of last resort" refer to?

  • An antibiotic that is only effective against a specific type of bacteria.
  • An antibiotic that is more effective than other antibiotics.
  • An antibiotic that is typically used as a first-line treatment.
  • An antibiotic that is only used when other antibiotics have failed. (correct)
  • What is the main idea of the passage?

    <p>To illustrate the dangers of antibiotic resistance and the need for alternative treatments. (D)</p> Signup and view all the answers

    What is the likely implication of "four people die every hour from an antibiotic-resistant bacterial infection in the United States"?

    <p>Antibiotic resistance is a growing threat to public health. (B)</p> Signup and view all the answers

    How does the shigella bacteria evade the effects of azithromycin?

    <p>It produces enzymes that neutralize the antibiotic molecules. (A)</p> Signup and view all the answers

    Which of these is NOT a symptom mentioned in the passage for the patient's shigella infection?

    <p>Nausea (D)</p> Signup and view all the answers

    What is the implication of the passage's focus on "bacteriophage therapy"?

    <p>Bacteriophage therapy is considered a promising alternative to antibiotics. (A)</p> Signup and view all the answers

    Which of the following statements accurately describes the impact of the Iron Curtain on the development of phage therapy?

    <p>Soviet researchers' work on phage therapy remained largely unknown to the rest of the world, hindering its global development. (A)</p> Signup and view all the answers

    What prompted the renewed interest in phage therapy in recent years?

    <p>The increasing number of antibiotic-resistant bacteria, posing a significant health threat. (A)</p> Signup and view all the answers

    What makes phages a potentially valuable tool in combating bacterial infections?

    <p>They target and destroy specific types of bacteria without harming beneficial bacteria. (B)</p> Signup and view all the answers

    Which of the following institutions is mentioned in the text as a pioneer in phage therapy?

    <p>The Hirszfeld Institute (A), The Eliava Institute (C)</p> Signup and view all the answers

    What is the term used to describe the collection of viruses that naturally live in and on humans?

    <p>Human virome (B)</p> Signup and view all the answers

    Why are phages sometimes called "living medicines"?

    <p>They are derived from living organisms and have biological activity. (C)</p> Signup and view all the answers

    What is the significance of the fact that phage medicines were sold in pharmacies in Tbilisi, Georgia?

    <p>It demonstrates that phage therapy is a well-established and widely accepted practice in some parts of the world. (B), It indicates that phage therapy has been used to treat a wide range of infections for a long time. (D)</p> Signup and view all the answers

    Which of the following is NOT mentioned as a potential benefit of phage therapy?

    <p>Prevention of antibiotic resistance development. (D)</p> Signup and view all the answers

    Which of the following is a valid reason why phage therapy is considered a promising alternative to antibiotics?

    <p>Phages are effective in treating infections that respond poorly to antibiotics. (B), Phages can target a wide range of bacteria, including antibiotic-resistant strains. (C)</p> Signup and view all the answers

    What was the primary purpose of the remedies made at Tbilisi's Institute of Bacteriophage, Microbiology, and Virology?

    <p>To treat sore throats (B)</p> Signup and view all the answers

    Who was the founder of the institute that developed phage remedies?

    <p>Giorgi Eliava (A)</p> Signup and view all the answers

    How were phages matched to patients at the institute?

    <p>Based on bacterial cultures from samples (B)</p> Signup and view all the answers

    What distinguishes phages from antibiotics according to the content provided?

    <p>Phages are personalized therapies and living medicines (D)</p> Signup and view all the answers

    What type of infections did patients at the institute commonly battle?

    <p>Stubborn skin and gut infections (D)</p> Signup and view all the answers

    Why were phages viewed with skepticism in the American medical establishment?

    <p>They were foreign and less reliable compared to antibiotics (B)</p> Signup and view all the answers

    What marked a significant shift in the FDA's view of phage therapy?

    <p>The approval of the first phage therapy trial in history (A)</p> Signup and view all the answers

    What process do antibiotics primarily use to eliminate bacteria?

    <p>Preventing the replication of bacterial cells (B)</p> Signup and view all the answers

    What is the composition of most phages that target human bacterial pathogens?

    <p>An icosahedral head with a protein tail (B)</p> Signup and view all the answers

    What occurs immediately after a lytic phage injects its genome into a bacterium?

    <p>The bacterial DNA and protein synthesis machinery are hijacked (D)</p> Signup and view all the answers

    What therapeutic potential was first described by Felix d'Hérelle in 1919?

    <p>The application of phage therapy to treat dysentery (B)</p> Signup and view all the answers

    What ultimately led to the decline in interest in phage therapy among Western scientists?

    <p>The widespread success of penicillin during and after World War II (A)</p> Signup and view all the answers

    What characterizes strictly lytic phages in their interaction with host bacteria?

    <p>They cause the host cell to reproduce phage and then kill it. (C)</p> Signup and view all the answers

    Who founded the Eliava Institute devoted to phage therapy research?

    <p>Giorgi Eliava (C)</p> Signup and view all the answers

    What is one of the main reasons phages are considered safe for mammalian cells?

    <p>They have evolved to only infect certain target bacteria. (C)</p> Signup and view all the answers

    How do phages reproduce after infecting a bacterial cell?

    <p>By using the bacterial machinery to produce copies (D)</p> Signup and view all the answers

    What is the primary function of the robot Neptune in the lab?

    <p>To automate the testing of phages against bacteria (B)</p> Signup and view all the answers

    What does Neptune primarily measure every half hour during its operation?

    <p>The population of bacterial cells in the solution (B)</p> Signup and view all the answers

    What kind of environment does Neptune create for the bacteria it tests?

    <p>A nutrient-rich soup with glucose and amino acids (D)</p> Signup and view all the answers

    Why has phage therapy research gained support from institutions like the National Institute of Allergy and Infectious Diseases?

    <p>Due to a rise in antibiotic-resistant bacterial strains (C)</p> Signup and view all the answers

    What is the significance of Neptune being able to extrapolate information for novel strains and phages?

    <p>It aids in predicting phage effectiveness against emerging bacterial threats. (A)</p> Signup and view all the answers

    How does Neptune create conditions for testing effectiveness against shigella?

    <p>By preloading with a specific nutrient solution found in the gut (C)</p> Signup and view all the answers

    What type of laboratory equipment does Neptune utilize to perform its tests?

    <p>A series of shallow tubes known as 96-well plates (D)</p> Signup and view all the answers

    What does Neptune do after loading the 96-well plates with bacteria and phages?

    <p>It places the plates into an incubator to monitor phage activity (A)</p> Signup and view all the answers

    How does shigella manage to evade the effects of colistin?

    <p>By changing its outer cell membrane structure (C)</p> Signup and view all the answers

    What do bacteriophages specifically target?

    <p>Bacterial infections (B)</p> Signup and view all the answers

    What is the significance of describing shigella as a 'superbug'?

    <p>It signifies its ability to resist multiple antibiotics (C)</p> Signup and view all the answers

    Why are bacteriophages considered a potential solution to antibiotic resistance?

    <p>They can specifically kill bacteria without affecting human cells (B)</p> Signup and view all the answers

    What is a significant consequence of the failure to control antibiotic-resistant infections?

    <p>Increased rate of complications and death from infections (D)</p> Signup and view all the answers

    Where are bacteriophages commonly found?

    <p>In locations where bacteria are prevalent (B)</p> Signup and view all the answers

    What does the phrase 'post-antibiotic era' imply?

    <p>Traditional antibiotics are becoming ineffective (D)</p> Signup and view all the answers

    How do bacteria typically respond to an antibiotic like colistin initially?

    <p>Sensitive bacteria are killed, but resistant ones thrive later (B)</p> Signup and view all the answers

    Flashcards

    Phage Therapy

    A treatment using bacteriophages to combat bacterial infections.

    Bacteriophages

    Viruses that specifically infect and destroy bacteria without harming human cells.

    Iron Curtain

    A metaphorical barrier that limited knowledge exchange during the Cold War, especially for Soviet research.

    Superbugs

    Bacteria that have become resistant to antibiotics, posing significant treatment challenges.

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    Eliava Institute

    A prominent center in Georgia known for pioneering phage therapy research and application.

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    Human Virome

    The collection of viruses present in and on the human body, including beneficial phages.

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    Microbiome

    The community of microbes that live in and on our bodies, crucial for health.

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    Living Medicines

    A term used to describe phages as natural antiviral agents in health treatment.

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    Kidney failure

    A medical condition where the kidneys lose their ability to function properly.

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    Colistin

    An antibiotic used to treat infections caused by certain bacteria.

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    Antibiotic resistance

    When bacteria evolve to resist the effects of antibiotics.

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    Ubiquitous

    Present everywhere; especially in relation to phages in the environment.

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    Trojan horse (in context)

    A strategy used by bacteriophages to infiltrate bacteria.

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    Post-antibiotic era

    A period where common infections can no longer be treated effectively with antibiotics.

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    Giorgi Eliava

    A Soviet scientist who founded the Eliava Institute known for phage research.

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    Personalized medicine

    Tailoring medical treatment to the individual characteristics of each patient.

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    FDA approval

    The process for the U.S. Food and Drug Administration to authorize a treatment for public use.

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    Stubborn infections

    Infections that do not respond quickly to standard treatments.

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    Comparison to antibiotics

    Phages are living medicines, unlike static antibiotics that kill bacteria indiscriminately.

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    Bacteriophage Therapy

    A treatment that uses viruses to target and destroy bacteria.

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    Shigella

    A genus of bacteria that can cause gastrointestinal infections, leading to bloody diarrhea.

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    Azithromycin

    An antibiotic commonly used to treat bacterial infections like stomach bugs.

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    Ciprofloxacin

    A broad-spectrum antibiotic used for more severe infections when first-line treatments fail.

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    Gastrointestinal Infection

    An infection affecting the stomach and intestines, often causing diarrhea and vomiting.

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    Neptune

    A biotech robot used for microbial warfare testing.

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    96-well plate

    A set of shallow tubes used to grow bacteria and phages.

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    Microbial warfare

    The process of testing interactions between bacteria and phages.

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    Artificial intelligence in phage research

    AI aids in predicting effective phage combinations.

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    Clinical trials for phage therapy

    Tests to evaluate the effectiveness of phages against bacteria.

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    Icosahedral head

    The geometric structure of certain phages with 20 flat faces and protein composition.

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    Lytic phage

    A type of bacteriophage that causes the host bacterium to lyse or burst after replication.

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    Phage genome injection

    The process where a phage injects its genetic material into a bacterium's periplasm.

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    Bacterial hijacking

    When a phage takes control of bacterial machinery to replicate itself.

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    Phage progeny

    The offspring of a phage, often numbering in the hundreds, that emerge after lysis.

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    Frederick Twort

    An English bacteriologist known for early discoveries of antibacterial viral agents.

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    Felix d'Hérelle

    A microbiologist credited with identifying the therapeutic potential of phages in 1919.

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    Study Notes

    Bacteriophage Therapy

    • Bacteriophages (phages) are viruses that infect and kill bacteria.
    • Phages are abundant in water, soil, and sewage.
    • Phages have been used to treat bacterial infections for over 100 years, but interest waned with the development of antibiotics.
    • Phages are specific to target bacteria, unlike antibiotics that are broad-spectrum.
    • Phage therapy is showing renewed interest due to rising antibiotic resistance.
    • Phages inject their DNA into bacteria, hijacking the bacterial machinery to create more phages, eventually killing the bacteria.
    • Phages have specialized "tails" for injecting genetic material into bacteria.

    Antibiotic Resistance

    • Antibiotic resistance is a major problem globally.
    • Four people die from antibiotic-resistant bacterial infections every hour in the U.S.
    • Bacteria can develop resistance to antibiotics rapidly through genetic mutations or via enzymes that destroy the antibiotics.

    Shigella Infections

    • Shigella is a bacteria that causes severe diarrheal illness.
    • Some strains of shigella are resistant to multiple antibiotics.
    • Symptoms can include severe diarrhea that can progress to bloody diarrhea.
    • Antibiotic treatment may not be effective in resistant cases.

    Phage Therapy Process

    • Researchers use sophisticated robotics (e.g., Neptune) to identify suitable phages for specific bacteria strains.
    • The process involves identifying the bacteria that causes the infection.
    • The researchers then match the appropriate phages to the bacteria, growing them in a medium with the target bacteria.
    • The optimal phage strains are tested with the target bacteria in various concentrations to assess their effectiveness and efficiency.
    • Phages are harvested from environments like sewage, which are rich in bacteria.

    Advantages of Phage Therapy

    • Phage therapy targets only the harmful bacteria, leaving beneficial gut bacteria unaffected.
    • Phages replicate rapidly, outcompeting the bacteria.
    • Phages are more specific to bacteria, thus potentially causing fewer side effects.

    Phage Therapy Research and Development

    • There are at least 50 phage clinical trials in progress.
    • Phage startups are emerging.
    • Organizations like the National Institute of Allergy and Infectious Diseases are supporting research in phage therapy.

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    Description

    Explore the world of bacteriophage therapy and its role in combating antibiotic-resistant bacteria. This quiz covers how phages work, their specificity, and the significance of addressing antibiotic resistance, particularly in infections like those caused by Shigella. Test your knowledge on these crucial topics in microbiology and infectious diseases.

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