Bacteriology Quiz
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Questions and Answers

Which classification does the genus Vibrio belong to?

  • Schizophytes
  • Schizomycetaceae (correct)
  • Pseudomonadales
  • Schizomyces

True sexual reproduction is common in bacteria.

False (B)

What is the size range of most bacteria?

0.2µm – 2µm

The smallest bacterium is called __________.

<p>Dialister pneumosintes</p> Signup and view all the answers

Which type of bacteria form irregular clusters that resemble grapes?

<p>Staphylococcus (B)</p> Signup and view all the answers

Bacteria are cosmopolitan in nature and can survive in extreme environments.

<p>True (A)</p> Signup and view all the answers

Name one plant-like characteristic of bacteria.

<p>They have a cell wall.</p> Signup and view all the answers

Match the following types of bacteria to their shapes:

<p>Coccus = Round or oval shape Bacillus = Rod shape Spirillum = Spiral shape Vibrio = Comma shape</p> Signup and view all the answers

Which type of bacteria has flagella distributed evenly throughout the cell surface?

<p>Peritrichous (A)</p> Signup and view all the answers

The slime layer mainly protects bacterial cells from desiccation and viral attacks.

<p>True (A)</p> Signup and view all the answers

What is the main function of the bacterial cell wall?

<p>To provide shape and protection against mechanical injury and pathogens.</p> Signup and view all the answers

The plasma membrane is __________, allowing selective permeability.

<p>flexible</p> Signup and view all the answers

Match the following bacterial structures with their functions:

<p>Slime layer = Protects against desiccation and viral attacks Cell wall = Provides shape and protection from injuries Plasma membrane = Selectively permeable barrier Mesosomes = Contain enzymes for electron transport</p> Signup and view all the answers

Which bacterial structure is responsible for maintaining osmotic regulation?

<p>Plasma membrane (D)</p> Signup and view all the answers

Bacteria can be considered multicellular organisms.

<p>False (B)</p> Signup and view all the answers

What is the primary composition of the bacterial cell wall?

<p>Peptidoglycan</p> Signup and view all the answers

What is a primary component of the cell wall in Gram-positive bacteria?

<p>Peptidoglycan (A)</p> Signup and view all the answers

Flagella in Gram-negative bacteria have two rings in their basal body.

<p>False (B)</p> Signup and view all the answers

What type of reproduction do bacteria primarily use?

<p>binary fission</p> Signup and view all the answers

In bacteria, the process of __________ results in the formation of two daughter cells.

<p>binary fission</p> Signup and view all the answers

Match the following types of bacteria with their examples:

<p>Staphylococcus = Gram-positive bacteria Escherichia = Gram-negative bacteria Bacillus = Gram-positive bacteria Klebsiella = Gram-negative bacteria</p> Signup and view all the answers

What role do porin proteins play in Gram-negative bacteria?

<p>Exchange of nutrients (B)</p> Signup and view all the answers

Mesosomes are more prominent in Gram-negative bacteria compared to Gram-positive bacteria.

<p>False (B)</p> Signup and view all the answers

During bacterial growth, what occurs after DNA replication?

<p>Development of constriction in the cell</p> Signup and view all the answers

What is the term used to describe the graphical representation of bacterial cell growth over time?

<p>Bacterial growth curve (A)</p> Signup and view all the answers

Exponential growth represents a small part of the bacterial life cycle.

<p>True (A)</p> Signup and view all the answers

What are the four characteristic phases of a typical bacterial growth curve?

<p>Lag Phase, Exponential (log) Phase, Stationary Phase, Death Phase</p> Signup and view all the answers

Some bacteria convert atmospheric nitrogen into nitrogenous compounds through a process called __________.

<p>nitrogen fixation</p> Signup and view all the answers

Which process involves the conversion of ammonia into nitrites and nitrates?

<p>Nitrification (C)</p> Signup and view all the answers

Bacillus subtilis is involved in the process of nitrification.

<p>False (B)</p> Signup and view all the answers

Name one beneficial use of bacteria in agriculture.

<p>Nitrogen fixation</p> Signup and view all the answers

Match the following processes with their corresponding descriptions:

<p>Nitrogen fixation = Conversion of atmospheric nitrogen into soil nutrients Ammonification = Conversion of proteins into ammonia Nitrification = Conversion of ammonia into nitrites and nitrates Bacterial growth curve = Graphical representation of bacterial cell growth</p> Signup and view all the answers

Which antibiotic is derived from Streptomyces ramous?

<p>Tetramycin (A)</p> Signup and view all the answers

Antiserum is used to treat or protect against specific diseases.

<p>True (A)</p> Signup and view all the answers

What type of vaccine is the BCG vaccine?

<p>living vaccine</p> Signup and view all the answers

Bacteria like ________ produce Vitamin B12.

<p>Bacillus megathermis</p> Signup and view all the answers

Match the following diseases with their causative bacteria:

<p>Ring rot of potato = Corynebacterium sepedonicum Black rot of cabbage = Xanthomonas campestris Wound infection = Proteus sp. Pneumonia = Streptococcus pneumoniae</p> Signup and view all the answers

Which bacterium is used for the production of human insulin (humulin)?

<p>Escherichia coli (B)</p> Signup and view all the answers

Lactobacillus acidophilus creates an alkaline environment in the vagina.

<p>False (B)</p> Signup and view all the answers

Cholera vaccine is classified as a ________ vaccine.

<p>killed vaccine</p> Signup and view all the answers

Which bacteria are responsible for causing pneumonia?

<p>Diplococcus pneumoniae (A)</p> Signup and view all the answers

Cyanobacteria are a type of eukaryotic microorganism.

<p>False (B)</p> Signup and view all the answers

What is the process called that reduces soil fertility by converting nitrites to atmospheric free nitrogen?

<p>denitrification</p> Signup and view all the answers

The bacterium responsible for cholera is called __________.

<p>Vibrio cholerae</p> Signup and view all the answers

Match the following bacteria with their associated diseases:

<p>Clostridium tetani = Tetanus Mycobacterium tuberculosis = Tuberculosis Clostridium perfringens = Gas gangrene Staphylococcus aureus = Staphylococcal food poisoning</p> Signup and view all the answers

Which bacteria are commonly associated with food spoilage in curd?

<p>Clostridium (D)</p> Signup and view all the answers

Bacteria like Bacillus subtilis contribute to increasing soil fertility by converting nitrogen.

<p>False (B)</p> Signup and view all the answers

What structure do cyanobacteria form that is referred to as trichomes?

<p>filaments</p> Signup and view all the answers

Flashcards

What is the most common way that bacteria reproduce?

Bacteria reproduce asexually through binary fission, where a single cell divides into two identical daughter cells.

What is the purpose of conjugation in bacteria?

Conjugation is a process of genetic recombination in bacteria where DNA is transferred from one bacterium to another through a pilus.

What are the plant-like characteristics of bacteria?

Bacteria share some characteristics with plants, such as having a cell wall, absorbing food in solution form, some species forming filaments, and producing their own food using CO2.

What is the size range of bacteria?

Bacteria are microscopic, unicellular organisms that range in size from 0.2µm to 2µm in diameter.

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What is a 'coccus' bacteria?

Coccus bacteria are round or oval shaped and can exist as single cells or in clusters.

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What is a 'diplococcus' bacteria?

Diplococci are coccus bacteria that remain in pairs.

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What is a 'streptococcus' bacteria?

Streptococci are coccus bacteria arranged in chains.

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What is a 'staphylococcus' bacteria?

Staphylococci are coccus bacteria arranged in irregular clusters resembling bunches of grapes.

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Slime layer

A gelatinous layer secreted by bacteria, protecting it from dehydration, antibodies, and pathogens.

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Capsule

A thicker slime layer, often found in virulent bacteria, providing extra protection against harmful elements.

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Cell wall

The tough, rigid outer layer of a bacterial cell, composed of peptidoglycan, providing shape and protection.

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Peptidoglycan

The main component of the bacterial cell wall, providing its strength and structure.

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Plasma membrane

A thin, flexible membrane inside the cell wall, controlling what enters and exits the cell.

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Mesosomes

Finger-like infoldings of the plasma membrane, containing enzymes for respiration and cell division.

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Cytoplasm

The gel-like substance within a bacterial cell, containing ribosomes for protein synthesis.

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Ribosomes

Tiny structures in the cytoplasm responsible for protein synthesis.

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What is peptidoglycan?

Peptidoglycan is a complex polymer found in bacterial cell walls. It's a tough, rigid layer that gives the cell shape and protection.

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What is the difference between Gram-positive and Gram-negative bacteria based on cell wall composition?

Gram-positive bacteria have a thick layer of peptidoglycan in their cell wall, while Gram-negative bacteria have a thinner peptidoglycan layer and an outer membrane containing lipopolysaccharide.

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What are porin proteins and where are they found?

Porin proteins are channel-like proteins that allow small molecules to pass through the outer membrane of Gram-negative bacteria, some Gram-positive Mycobacteria, and mitochondrial and chloroplast membranes.

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What is binary fission?

Binary fission is the primary mode of reproduction in bacteria. It involves a single cell dividing into two identical daughter cells.

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What are the stages of bacterial growth?

Bacterial growth involves four stages: Lag phase (preparation), Log phase (rapid growth), Stationary phase (stable population), and Death phase (decline in population).

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What is the 'Log phase' of bacterial growth?

The log phase is a period of exponential growth in a bacterial population. Each bacterium divides into two, and those two divide again, leading to rapid increase.

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What is the 'Stationary phase' in bacterial growth?

The stationary phase occurs when bacterial growth slows down and the number of new bacteria equals the number of dying bacteria. Resources become limited.

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What is the 'Death phase' in bacterial growth?

The death phase is when resources become depleted and the bacterial population begins to decline. More bacteria are dying than being produced.

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What is the bacterial growth curve?

A graph that shows the number of living bacterial cells in a population over time. It illustrates the different phases of bacterial growth: Lag Phase, Exponential Phase, Stationary Phase, and Death Phase.

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Lag Phase - What is the bacterial growth like in this phase?

Bacteria are adjusting to their new environment. They are metabolically active but are not yet dividing. This phase is characterized by a slow or no increase in population.

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Exponential (Log) Phase - What is the bacterial growth like in this phase?

Bacteria divide at their maximum rate, doubling in number with each generation. This phase is characterized by exponential growth, where population increases rapidly.

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Stationary Phase - What is the bacterial growth like in this phase?

The rate of cell division equals the rate of cell death. The population remains relatively stable because factors like nutrient depletion and waste accumulation limit growth.

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Death Phase - What is the bacterial growth like in this phase?

The number of bacteria dying exceeds the number dividing. This phase is characterized by a decline in population due to unfavorable conditions like nutrient depletion and accumulation of toxic waste.

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Why is Nitrogen Fixation important for agriculture?

Bacteria convert nitrogen from the atmosphere into forms that plants can use. This process enriches the soil and promotes plant growth, increasing agricultural productivity.

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What is Ammonification?

Bacteria break down dead organisms and waste products into ammonia, a form of nitrogen that plants can absorb.

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What is Nitrification?

Bacteria convert ammonia into nitrites and then nitrates, essential nutrients for plant growth.

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What are Cyanobacteria?

Cyanobacteria, also known as blue-green algae, are photosynthetic prokaryotes with unique characteristics like a prokaryotic cellular structure, omnipresence, and a diverse range of pigments.

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What are trichomes?

Trichomes are filaments of Cyanobacteria cells held together by a sheath. They are like long chains of individual cells.

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What are the characteristic pigments of Cyanobacteria?

Cyanobacteria contain a variety of pigments like chlorophyll-a, carotenoids (like beta-carotene), and phycobilins (like phycocyanin and phycoerythrin).

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What are the storage products of Cyanobacteria?

Cyanobacteria store energy as cyanophycean starch and protein. These are their food reserves.

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Do Cyanobacteria have flagella?

No, Cyanobacteria lack flagella (tail-like structures used for movement). They move by gliding or using gas vesicles.

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What bacteria cause gas gangrene?

Clostridium perfringens bacteria cause gas gangrene, a serious infection in tissues.

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What bacteria cause staphylococcal food poisoning?

Staphylococcus aureus bacteria are responsible for staphylococcal food poisoning, a common foodborne illness.

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How does denitrification reduce soil fertility?

Denitrifying bacteria like Bacillus denitrificans convert nitrites in the soil to atmospheric nitrogen gas, reducing the amount of nitrogen available to plants, thus lowering soil fertility.

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What are antibiotics?

Antibiotics are substances that kill or inhibit the growth of bacteria. They are often used to treat bacterial infections in humans and animals.

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What are some examples of antibiotics and the bacteria they are made from?

Tetramycin is produced by Streptomyces ramous, Chloramphenical is produced by Streptomyces venezuelae, Bacitracin is produced by Bacillus licheniformis, Subtilin is produced by Bacillus subtilis, and Polymyxin B is produced by Bacillus polymyxa.

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What is an antiserum?

An antiserum is a blood serum containing antibodies against specific antigens. It is used to treat or protect against specific diseases. Antiserum obtained from bacterial cells is used in the treatment of meningitis, pneumonia, and diphtheria.

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What is a vaccine?

A vaccine is a substance containing weakened or dead microorganisms or their parts that is used to stimulate an immune response and provide immunity against specific diseases. Cholera vaccine is a killed vaccine used for the treatment of cholera, BCG (Bacillus Calmette-Guerin) is a living vaccine used for the treatment of Tuberculosis, TT vaccine is used for tetanus, and TAB vaccine is used for typhoid.

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How are vitamins produced using bacteria?

Certain bacteria can be used to produce vitamins. For example, Clostridium butylicum produces vitamin B2, Bacillus megathermis produces vitamin B12, Acetobacter aceti produces vitamin C, and Escherichia coli produces vitamins B and K.

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How does Lactobacillus acidophilus protect the vagina?

Lactobacillus acidophilus grows in the vagina, creating an acidic environment that protects it from harmful microorganisms.

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What is humulin and how is it produced?

Humulin is human insulin produced through genetic engineering of Escherichia coli bacteria.

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What are some examples of diseases caused by bacteria?

Bacteria can cause various diseases in plants and animals. For example, Corynebacterium sepedonicum causes ring rot of potato, Xanthomonas campestris causes black rot of cabbage, and Proteus sp. causes wound infections in animals.

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Study Notes

Kingdom Monera

  • Kingdom Monera encompasses prokaryotic, simple, microscopic organisms inhabiting various environments.
  • It was classified by Stanier and Van Neil, and is one of the five kingdom classifications proposed by Whitaker (1969).
  • Organisms within Monera exhibit primitive, unicellular structures.

General Characteristics of Monera

  • Monera organisms are prokaryotic and unicellular.
  • They lack a membrane-bound nucleus but possess a nucleoid containing DNA, RNA, and proteins.
  • Their DNA is double-stranded and circular, without histone proteins.
  • They typically lack membrane-bound organelles like mitochondria, plastids, and Golgi bodies.
  • Their cell walls are rigid.
  • They are cosmopolitan in distribution, meaning they are found in diverse habitats.
  • Their nutrition encompasses both autotrophic and heterotrophic modes. Autotrophs can create their own food, while heterotrophs obtain nutrients, including saprophytes which feed on dead organic matter or parasites which derive nutrition from a host. Symbiosis is a mutual relationship for survival where either one organism receives a benefit, while commensalism involves one organism benefitting and the other organism remaining unaffected.

Respiration in Monera

  • Respiration in Monera can be categorized as obligate aerobes, obligate anaerobes, and facultative anaerobes. Obligate aerobes need oxygen to survive, while obligate anaerobes cannot survive in the presence of oxygen. Facultative anaerobes can survive in either presence or absence of oxygen.

Classification of Monera

  • Monera is classified into two major groups: Eubacteria and Archaebacteria.
  • Archaebacteria represent ancient bacteria adapted to extreme environments.
  • Eubacteria are the true bacteria found in normal conditions.
  • More recent classifications are based on ribosomal RNA sequences which resulted in a modern classification into three domains: Archaea, Bacteria, and Eukarya. These include Kingdom Archaebacteria, Kingdom Eubacteria, and Kingdoms Protista, Plantae, Fungi, and Animalia.

Morphology of Bacteria

  • Bacteria, based on their shape, are classified into several types: coccus (spherical), bacillus (rod-shaped), vibrio (comma-shaped), spirilla (spiral-shaped).
  • These forms may occur singly, in pairs (diplo-), in groups of four (tetra-), in chains (strepto-), or clusters (staphylo-)
  • Other unique forms include filamentous, stalked, and pleomorphic bacteria which adapt their shape to the surrounding environment.

Bacterial Cell Structure

  • Bacteria lack membrane-bound organelles such as mitochondria, endoplasmic reticulum, chloroplasts.
  • Bacterial cell walls are composed of peptidoglycan.
  • Bacterial cells may also be enclosed within a slime layer or capsule, offering protection from desiccation, and antibodies.
  • Cell membranes are located beneath the cell wall.
  • The cytoplasm includes ribosomes, genetic material, and storage granules.
  • Mesosomes are infoldings of the plasma membrane that are important in cellular processes, including respiration.
  • Some bacterial cells have flagella that assist in movement.
  • Protoplasmic structures aid in nutrient storage or protein synthesis.

Reproduction in Bacteria

  • Bacteria primarily reproduce asexually via binary fission.
  • In some cases, genetic material exchange, or recombination, may occur via conjugation, transformation, or transduction.

Mode of Nutrition

  • Bacteria are classified based on their method of obtaining energy and carbon.
  • Autotrophic bacteria make their own food, and can be photoautotrophic—harvesting light energy— or chemoautotrophic—obtaining energy from chemical reactions.
  • Heterotrophic bacteria cannot make their own food, extracting nutrients from organic substances produced by organisms. Saprophytic bacteria derive nutrition from dead and decaying matter, while symbiotic bacteria benefit other organisms in their vicinity. Parasitic bacteria rely on a host and may or may not cause disease.

Economic Importance of Bacteria (including Monera)

  • Agricultural Importance: Bacteria are crucial in nitrogen fixation, ammonification, nitrification, and decomposition processes, impacting soil fertility and nutrient cycling. Bacteria are crucial in sewage treatment and composting.
  • Industrial Importance: Bacteria are integral to fermenting dairy products such as yogurt and cheese, in the production of organic acids, alcohol, and enzymes.
  • Medical Importance:
    • Bacteria are used to produce antibiotics, as well as some vaccines.
    • Bacteria can cause diseases, such as pneumonia, cholera, and tuberculosis, and can pose a risk to both human and animal health.
    • Antisera help combat specific diseases.
  • Harmful Importance:
  • Bacteria can cause spoilage or damage to food and agricultural products, and can cause various diseases in diverse organisms.
  • Some bacteria cause food poisoning, reduce soil fertility, and cause damage to various materials.

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Description

Test your knowledge on various aspects of bacteriology, including bacterial classification, structures, and functions. This quiz will challenge your understanding of bacterial characteristics and their survival mechanisms in different environments.

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