40 Questions
Which of the following is a characteristic of Listeria monocytogenes?
Gram positive rod or coccobacillus
What is the presumptive test for Listeria monocytogenes motility?
Umbrella-like or inverted Christmas tree motility on semi-solid media
Which of the following is a disease associated with Listeria monocytogenes?
Neonatal meningitis
What is the laboratory diagnosis method for Listeria monocytogenes?
Cold enrichment at 4°C and culture on McBride agar
What is the most common egg-based media for Mycobacterium tuberculosis?
Lowenstein-Jensen
Which staining method can identify Mycobacteria up to genus level?
Ziehl-Neelsen
What does DOTS stand for in the context of Mycobacterium?
Directly Observed Treatment Strategy
Which type of media provides faster results for Mycobacterium?
Liquid culture media like Bactec 12B and 13A
What is the recommended media for heavily contaminated specimens according to the American Thoracic Society?
Petragnani
Which staining method uses Auramine rhodamine stain and KMN04 counterstain?
Fluorochrome staining
What type of specimens require decontamination and digestion?
Sputum
Which media includes vitamins, glycerol, and malachite green for Mycobacterium?
Serum-agar based media
What is used for liquefying and decontaminating sputum specimens?
NaOH and NALC
What test aids in the identification of non-TB Mycobacteria?
Test for pigment production
Which culture media is recommended for culture of Mycobacterium tuberculosis lasting 6-12 months?
Non-selective egg-based media
What staining method is used for easier reading of Mycobacteria?
Fluorochrome staining
What is the primary reason for Mycobacterium being acid-fast?
Presence of mycolic acid
Which type of TB is associated with resistance to primary TB drugs and fluoroquinolones?
XDR-TB
What is the causative agent of leprosy?
Mycobacterium leprae
Which test is used for the diagnosis of M. leprae?
Wade Fite Faraco test
How is Mycobacterium bovis transmitted to humans?
Ingestion of contaminated milk
Which mycobacterium is associated with human cases of TB in tropical Africa?
Mycobacterium canetti
What is the primary characteristic of Mycobacterium tuberculosis?
Obligate aerobe
Which type of leprosy is associated with different immune responses and diagnostic methods?
Lepromatous
What is the primary method of transmission for Mycobacterium leprae?
Contact with infected skin
Which group does Mycobacterium tuberculosis belong to?
Tuberculosis complex
What are the types of TB mentioned in the text?
Primary, secondary, MDR-TB, XDR, Pott’s disease, granuloma
What are the susceptibility tests mentioned for TB diagnosis?
Serpentine cord formation and Mantoux test
Which Mycobacterium species is known as the 'YELLOW BACILLUS'?
Mycobacterium kansasii
Which Mycobacterium species requires hemin for growth?
Mycobacterium haemophilum
Which Mycobacterium species is associated with skin infections occurring as red to blue lesions and swimming pool granuloma?
Mycobacterium marinum
Which Mycobacterium species is first isolated from MACACA RHESUS MONKEY?
Mycobacterium simiae
Which Mycobacterium species is known as the 'TAP WATER BACILLUS'?
Mycobacterium gordonae
Which Mycobacterium species can cause arthritis and osteomyelitis?
Mycobacterium terrae
Which test is used to detect the production of NITROREDUCTASE in Mycobacterium species?
Nitrate test
Which biochemical test aids in the identification of non-TB Mycobacteria?
Arylsulfatase test
Which Mycobacterium species optimally grows at 42 degC and forms branching colonies with aerial hyphae on cornmeal agar?
Mycobacterium xenopi
Which Mycobacterium species is associated with the production of a (+) arylsulfatase?
Mycobacterium fortuitum
Which Mycobacterium species is known as the 'BATTEY BACILLUS'?
Mycobacterium intracellulare
Which Mycobacterium species is similar to M. simiae but negative for niacin?
Mycobacterium asiaticum
Study Notes
Bacteriology Acid-Fast Bacilli Lecture Summary
- Mycobacterium is acid-fast due to mycolic acid, slow-growing, and requires whole egg for growth
- Three groups of mycobacteria: tuberculosis complex, leprae, and other than tuberculosis
- Mycobacterium tuberculosis is known as Koch’s Bacillus, with "MUCH GRANULES" and obligate aerobe
- Types of TB include primary, secondary, MDR-TB, XDR, Pott’s disease, and granuloma
- Primary TB forms tubercles, caseation lesions, and Ghon complexes; secondary TB is a repeat of previous infection
- MDR-TB shows resistance to primary drugs, while XDR shows resistance to primary TB drugs and fluoroquinolones
- Treatment for TB includes primary and secondary drugs, with susceptibility tests such as serpentine cord formation and Mantoux test
- Mycobacterium bovis causes TB in animals and humans, transmitted through ingestion of contaminated milk or exposure to infected animals
- Mycobacterium canetti grows more rapidly than M. tuberculosis and is associated with human cases of TB in tropical Africa
- Mycobacterium leprae causes leprosy and is non-culturable on artificial media, transmitted through inhalation or contact with infected skin
- Leprosy has two forms: tuberculoid and lepromatous, with different immune responses and diagnostic methods
- Laboratory diagnosis of mycobacteria involves skin tests, lepra cells, and basis of diagnosis, with the Wade Fite Faraco test and phenolase test for M. leprae
Test your knowledge of bacteriology and acid-fast bacilli with this comprehensive lecture summary quiz. Explore key concepts such as Mycobacterium tuberculosis, types of tuberculosis, drug resistance, treatment, and the causative agent of leprosy. Dive into laboratory diagnosis methods and different strains of mycobacteria.
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