Bacteriology and Acid-Fast Bacilli Quiz

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Questions and Answers

Which of the following is a characteristic of Listeria monocytogenes?

Non-motile at room temperature

Gram negative spore-forming bacillus

Gram positive rod or coccobacillus (correct)

Strict anaerobe

What is the presumptive test for Listeria monocytogenes motility?

Spiral motility on agar plates

No motility observed on any media

Umbrella-like or inverted Christmas tree motility on semi-solid media (correct)

Random and erratic motility on wet mounts

Which of the following is a disease associated with Listeria monocytogenes?

Gastroenteritis in young adults

Skin infections in immunocompromised individuals

Pneumonia in elderly patients

Neonatal meningitis (correct)

What is the laboratory diagnosis method for Listeria monocytogenes?

Cold enrichment at 4°C and culture on McBride agar Signup and view all the answers

What is the most common egg-based media for Mycobacterium tuberculosis?

Lowenstein-Jensen Signup and view all the answers

Which staining method can identify Mycobacteria up to genus level?

Ziehl-Neelsen Signup and view all the answers

What does DOTS stand for in the context of Mycobacterium?

Directly Observed Treatment Strategy Signup and view all the answers

Which type of media provides faster results for Mycobacterium?

Liquid culture media like Bactec 12B and 13A Signup and view all the answers

What is the recommended media for heavily contaminated specimens according to the American Thoracic Society?

Petragnani Signup and view all the answers

Which staining method uses Auramine rhodamine stain and KMN04 counterstain?

Fluorochrome staining Signup and view all the answers

What type of specimens require decontamination and digestion?

Sputum Signup and view all the answers

Which media includes vitamins, glycerol, and malachite green for Mycobacterium?

Serum-agar based media Signup and view all the answers

What is used for liquefying and decontaminating sputum specimens?

NaOH and NALC Signup and view all the answers

What test aids in the identification of non-TB Mycobacteria?

Test for pigment production Signup and view all the answers

Which culture media is recommended for culture of Mycobacterium tuberculosis lasting 6-12 months?

Non-selective egg-based media Signup and view all the answers

What staining method is used for easier reading of Mycobacteria?

Fluorochrome staining Signup and view all the answers

What is the primary reason for Mycobacterium being acid-fast?

Presence of mycolic acid Signup and view all the answers

Which type of TB is associated with resistance to primary TB drugs and fluoroquinolones?

XDR-TB Signup and view all the answers

What is the causative agent of leprosy?

Mycobacterium leprae Signup and view all the answers

Which test is used for the diagnosis of M. leprae?

Wade Fite Faraco test Signup and view all the answers

How is Mycobacterium bovis transmitted to humans?

Ingestion of contaminated milk Signup and view all the answers

Which mycobacterium is associated with human cases of TB in tropical Africa?

Mycobacterium canetti Signup and view all the answers

What is the primary characteristic of Mycobacterium tuberculosis?

Obligate aerobe Signup and view all the answers

Which type of leprosy is associated with different immune responses and diagnostic methods?

Lepromatous Signup and view all the answers

What is the primary method of transmission for Mycobacterium leprae?

Contact with infected skin Signup and view all the answers

Which group does Mycobacterium tuberculosis belong to?

Tuberculosis complex Signup and view all the answers

What are the types of TB mentioned in the text?

Primary, secondary, MDR-TB, XDR, Pott’s disease, granuloma Signup and view all the answers

What are the susceptibility tests mentioned for TB diagnosis?

Serpentine cord formation and Mantoux test Signup and view all the answers

Which Mycobacterium species is known as the 'YELLOW BACILLUS'?

Mycobacterium kansasii Signup and view all the answers

Which Mycobacterium species requires hemin for growth?

Mycobacterium haemophilum Signup and view all the answers

Which Mycobacterium species is associated with skin infections occurring as red to blue lesions and swimming pool granuloma?

Mycobacterium marinum Signup and view all the answers

Which Mycobacterium species is first isolated from MACACA RHESUS MONKEY?

Mycobacterium simiae Signup and view all the answers

Which Mycobacterium species is known as the 'TAP WATER BACILLUS'?

Mycobacterium gordonae Signup and view all the answers

Which Mycobacterium species can cause arthritis and osteomyelitis?

Mycobacterium terrae Signup and view all the answers

Which test is used to detect the production of NITROREDUCTASE in Mycobacterium species?

Nitrate test Signup and view all the answers

Which biochemical test aids in the identification of non-TB Mycobacteria?

Arylsulfatase test Signup and view all the answers

Which Mycobacterium species optimally grows at 42 degC and forms branching colonies with aerial hyphae on cornmeal agar?

Mycobacterium xenopi Signup and view all the answers

Which Mycobacterium species is associated with the production of a (+) arylsulfatase?

Mycobacterium fortuitum Signup and view all the answers

Which Mycobacterium species is known as the 'BATTEY BACILLUS'?

Mycobacterium intracellulare Signup and view all the answers

Which Mycobacterium species is similar to M. simiae but negative for niacin?

Mycobacterium asiaticum Signup and view all the answers

Study Notes

Bacteriology Acid-Fast Bacilli Lecture Summary

Mycobacterium is acid-fast due to mycolic acid, slow-growing, and requires whole egg for growth
Three groups of mycobacteria: tuberculosis complex, leprae, and other than tuberculosis
Mycobacterium tuberculosis is known as Koch’s Bacillus, with "MUCH GRANULES" and obligate aerobe
Types of TB include primary, secondary, MDR-TB, XDR, Pott’s disease, and granuloma
Primary TB forms tubercles, caseation lesions, and Ghon complexes; secondary TB is a repeat of previous infection
MDR-TB shows resistance to primary drugs, while XDR shows resistance to primary TB drugs and fluoroquinolones
Treatment for TB includes primary and secondary drugs, with susceptibility tests such as serpentine cord formation and Mantoux test
Mycobacterium bovis causes TB in animals and humans, transmitted through ingestion of contaminated milk or exposure to infected animals
Mycobacterium canetti grows more rapidly than M. tuberculosis and is associated with human cases of TB in tropical Africa
Mycobacterium leprae causes leprosy and is non-culturable on artificial media, transmitted through inhalation or contact with infected skin
Leprosy has two forms: tuberculoid and lepromatous, with different immune responses and diagnostic methods
Laboratory diagnosis of mycobacteria involves skin tests, lepra cells, and basis of diagnosis, with the Wade Fite Faraco test and phenolase test for M. leprae

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Description

Test your knowledge of bacteriology and acid-fast bacilli with this comprehensive lecture summary quiz. Explore key concepts such as Mycobacterium tuberculosis, types of tuberculosis, drug resistance, treatment, and the causative agent of leprosy. Dive into laboratory diagnosis methods and different strains of mycobacteria.