Bacterial Staining: Composition and Techniques

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the composition of basic dyes used for bacterial staining?

  • Colored negative ions
  • Uncolored negative ions
  • Uncolored positive ions
  • Colored positive ions (correct)

What is the purpose of passing the slide through a Bunsen burner flame during smear preparation?

  • To heat-kill and fix the organisms to the slide (correct)
  • To enhance the color of the stain
  • To sterilize the slide
  • To prevent bacterial growth on the slide

Which type of stain is used for negative staining in bacterial samples?

  • Neutral dye
  • Acidic dye (correct)
  • Basic dye
  • Fluorescent dye

What is the purpose of spreading the organisms over an area about â…” of the slide during smear preparation?

<p>To ensure even distribution of bacteria for staining (B)</p> Signup and view all the answers

Which type of stain consists of both colored negative and positive ions?

<p>Neutral dye (D)</p> Signup and view all the answers

Flashcards are hidden until you start studying

Study Notes

Basic Dyes

  • Basic dyes, used for bacterial staining, are composed of a colored positive ion (chromophore) and a negative ion (counter ion).

Smear Preparation

  • Passing the slide through a Bunsen burner flame during smear preparation is done to fix the bacterial cells to the slide, killing them and preventing them from being washed away during the staining process.
  • Spreading the organisms over an area about â…” of the slide during smear preparation helps to achieve a uniform single layer of bacteria, which is essential for accurate staining and microscopic observation.

Staining Techniques

  • Negative staining in bacterial samples is achieved using nigrosin or India ink, which allows the bacteria to appear as unstained objects against a dark background.
  • Stains that consist of both colored negative and positive ions are called acid-base indicators.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Quiz Team

More Like This

Use Quizgecko on...
Browser
Browser