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Questions and Answers
What is the composition of basic dyes used for bacterial staining?
What is the composition of basic dyes used for bacterial staining?
- Colored negative ions
- Uncolored negative ions
- Uncolored positive ions
- Colored positive ions (correct)
What is the purpose of passing the slide through a Bunsen burner flame during smear preparation?
What is the purpose of passing the slide through a Bunsen burner flame during smear preparation?
- To heat-kill and fix the organisms to the slide (correct)
- To enhance the color of the stain
- To sterilize the slide
- To prevent bacterial growth on the slide
Which type of stain is used for negative staining in bacterial samples?
Which type of stain is used for negative staining in bacterial samples?
- Neutral dye
- Acidic dye (correct)
- Basic dye
- Fluorescent dye
What is the purpose of spreading the organisms over an area about â…” of the slide during smear preparation?
What is the purpose of spreading the organisms over an area about â…” of the slide during smear preparation?
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Which type of stain consists of both colored negative and positive ions?
Which type of stain consists of both colored negative and positive ions?
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Study Notes
Basic Dyes
- Basic dyes, used for bacterial staining, are composed of a colored positive ion (chromophore) and a negative ion (counter ion).
Smear Preparation
- Passing the slide through a Bunsen burner flame during smear preparation is done to fix the bacterial cells to the slide, killing them and preventing them from being washed away during the staining process.
- Spreading the organisms over an area about â…” of the slide during smear preparation helps to achieve a uniform single layer of bacteria, which is essential for accurate staining and microscopic observation.
Staining Techniques
- Negative staining in bacterial samples is achieved using nigrosin or India ink, which allows the bacteria to appear as unstained objects against a dark background.
- Stains that consist of both colored negative and positive ions are called acid-base indicators.
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