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Bacterial Staining: Composition and Techniques

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Bacterial Staining: Composition and Techniques

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Questions and Answers

What is the composition of basic dyes used for bacterial staining?

Colored negative ions

Uncolored negative ions

Uncolored positive ions

Colored positive ions (correct)

What is the purpose of passing the slide through a Bunsen burner flame during smear preparation?

To heat-kill and fix the organisms to the slide (correct)

To enhance the color of the stain

To sterilize the slide

To prevent bacterial growth on the slide

Which type of stain is used for negative staining in bacterial samples?

Neutral dye

Acidic dye (correct)

Basic dye

Fluorescent dye

What is the purpose of spreading the organisms over an area about ⅔ of the slide during smear preparation?

<p>To ensure even distribution of bacteria for staining</p> Signup and view all the answers

Which type of stain consists of both colored negative and positive ions?

<p>Neutral dye</p> Signup and view all the answers

Study Notes

Basic Dyes

Basic dyes, used for bacterial staining, are composed of a colored positive ion (chromophore) and a negative ion (counter ion).

Smear Preparation

Passing the slide through a Bunsen burner flame during smear preparation is done to fix the bacterial cells to the slide, killing them and preventing them from being washed away during the staining process.
Spreading the organisms over an area about ⅔ of the slide during smear preparation helps to achieve a uniform single layer of bacteria, which is essential for accurate staining and microscopic observation.

Staining Techniques

Negative staining in bacterial samples is achieved using nigrosin or India ink, which allows the bacteria to appear as unstained objects against a dark background.
Stains that consist of both colored negative and positive ions are called acid-base indicators.

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Description

Test your knowledge of bacterial staining with questions about the composition and types of stains, smear preparation, and staining techniques. Learn about the importance of staining in making bacteria visible under a microscope.