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Bacterial Identification Methods Quiz

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Questions and Answers

Which method is used to detect Treponema pallidum in material from primary or secondary syphilitic lesions?

Saline mount

Hanging drop technique

Darkfield microscopy (correct)

Phase-contrast microscopy

What is the primary stain used in Acid Fast Stain?

Phenol

Heat

Methylene blue

Carbol fuchsin (correct)

What is the purpose of 10% potassium hydroxide (KOH) preparation?

To detect Treponema pallidum

To break down the tissue surrounding the fungal mycelia (correct)

To visualize bacteria with thick cell walls

To retain the crystal violet-iodine complex dye after decolorization

Which stain is used to differentiate bacteria based on cell wall characteristics?

Gram Stain Signup and view all the answers

What is the purpose of Phase-contrast microscopy?

To visualize unstained specimens Signup and view all the answers

What is the purpose of the hanging drop technique in bacterial identification?

To observe the motility of bacteria Signup and view all the answers

Which stain is used to differentiate bacteria based on the presence of teichoic acid in their cell walls?

Gram stain Signup and view all the answers

What is the primary purpose of 10% potassium hydroxide (KOH) preparation in bacterial identification?

To break down tissue surrounding fungal mycelia Signup and view all the answers

What is the main function of phase-contrast microscopy in bacterial identification?

To observe the motility of bacteria Signup and view all the answers

What is the primary stain used in Ziehl-Neelsen (acid-fast) stain?

Carbol fuchsin Signup and view all the answers