Bacterial Classification and Gram Staining Mechanism Quiz

Crystal violet (CV)

Alcohol increases the porosity of the cell wall, allowing the stain to wash off easily.

Gram's Iodine kills microbes and increases cells' affinity for the stain.

<p>To color Gram-negative bacteria pink and Gram-positive bacteria purple-black.</p> Signup and view all the answers

<p>Incorrect heat fixation can affect the number of bacteria present and their adhesion to the slide.</p> Signup and view all the answers

<p>Alcohol shrinks the pores of the cell wall, allowing the stain to be retained.</p> Signup and view all the answers

<p>Extensive damage to the cell membrane and cell wall</p> Signup and view all the answers

<p>As cultures age, especially gram positive cells, they tend to lose their ability to retain the primary stain and may appear gram variable.</p> Signup and view all the answers

<p>Older cultures may impact staining results by becoming gram variable.</p> Signup and view all the answers

<p>Stock culture collection is maintained for preservation of microbial cultures.</p> Signup and view all the answers

<p>Darker in color and could be mistaken for a light purple gram-positive stained cell.</p> Signup and view all the answers

<p>Timing of the decolorizing step is crucial as over decolorizing or decolorizing for too short a time period can impact staining results.</p> Signup and view all the answers

<p>Gram stain</p> Signup and view all the answers

<p>Dr. Hans Christian Gram</p> Signup and view all the answers

<ol> <li>Crystal Violet, 2. Gram's Iodine, 3. 95% Ethyl Alcohol, 4. Safranin</li> </ol> Signup and view all the answers

<p>Do not over-decolorize; add reagent drop by drop until the alcohol runs most clear, showing only a blue tinge.</p> Signup and view all the answers

<p>To get a closer look at the bacterial cells for classification</p> Signup and view all the answers

<p>It is an essential tool for classification and differentiation of microorganisms.</p> Signup and view all the answers