Bacteria Identification and Disinfection Quiz
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Questions and Answers

What is the main purpose of viewing microbes up close with light and electron microscopes?

  • To determine the exact number of microbes present on a surface
  • To remove all the microbes from a surface
  • To identify the types of microbes present on a surface (correct)
  • To clean and disinfect the surfaces

    • What is the role of a microbiologist in the detection of microscopic organisms?

  • Sterilizing medical devices
  • Culturing the microbes until they grow into a detectable form (correct)
  • Viewing microbes up close with microscopes
  • Cleaning and disinfecting surfaces

    • What is the first step in decontaminating the needle described in the text?

  • Cleaning it to remove any dirt, soil, and bacteria (correct)
  • Sterilizing the needle to kill all remaining microbes
  • Viewing the needle under high magnification
  • Putting it through a disinfection process

    • Why is it impossible to visually determine when all microbes have been removed during decontamination processes?

    <p>Due to the presence of bacteria sticking to the needle</p> Signup and view all the answers

    What is the significance of sterilization in the context of decontaminating medical devices?

    <p>To kill all remaining microbes on medical devices</p> Signup and view all the answers

    What is the primary reason for using a disinfection process on the needle?

    <p>To kill as many remaining microbes as possible</p> Signup and view all the answers

    What is the primary reason for performing disinfection or sterilization after the cleaning process?

    <p>To kill any microbes missed by the cleaning procedures</p> Signup and view all the answers

    Why is it important for specialists in Central Sterile Supply Department (CSSD) to know about microbiology and microbes?

    <p>To understand contamination risks and proper decontamination procedures</p> Signup and view all the answers

    What are the best studied microbes referred to as?

    <p>Pathogens</p> Signup and view all the answers

    Why is it becoming a serious problem when microbial infections can no longer be treated using antibiotics?

    <p>Because microbial infections are becoming more resistant to many or all available antibiotics</p> Signup and view all the answers

    What are the basic requirements for all microorganisms to grow and reproduce?

    <p>Food and moisture</p> Signup and view all the answers

    What is an example of a survival strategy used by bacteria under stressful conditions?

    <p>Altered metabolism</p> Signup and view all the answers

    Why is it important to clean reusable medical devices as soon as possible after use?

    <p>To avoid rapid multiplication of bacteria on the devices</p> Signup and view all the answers

    Why do some yeasts, like Candida, multiply as quickly as bacteria?

    <p>Because they have optimal growth conditions similar to human tissue</p> Signup and view all the answers

    What is the primary reason for understanding biofilms in the context of microbiology?

    <p>To realize that most microbes naturally grow as biofilms</p> Signup and view all the answers

    Why are enveloped viruses, such as Hepatitis B, HIV, and Herpes, more susceptible to protein, heat, and drying than non-enveloped viruses?

    <p>Because enveloped viruses have a lipid envelope</p> Signup and view all the answers

    What is the primary function of prions outside of host cells?

    <p>To remain biologically inert and persist for various lengths of time</p> Signup and view all the answers

    What is a characteristic feature of bacteria under optimal growth conditions?

    <p>They form spores and alter their metabolism</p> Signup and view all the answers

    What is the approximate rate at which the forearm sheds dead skin cells per hour?

    <p>1300 cells</p> Signup and view all the answers

    What is the primary purpose of using hygiene procedures and appropriate PPE in clean or sterile zones in the CSSD?

    <p>To prevent contamination of reusable medical devices</p> Signup and view all the answers

    What is the main reason for wearing two layers of clothing when entering critical areas?

    <p>To prevent contamination of the environment</p> Signup and view all the answers

    What is one of the requirements for PPE in the Decontamination Zone in CSSD?

    <p>Discarding used gloves before touching anything</p> Signup and view all the answers

    What is the purpose of wearing gloves in the Decontamination Zone?

    <p>To protect against contaminated reusable medical devices</p> Signup and view all the answers

    What is a requirement for workwear in the IAP or sterile storage zones in CSSD?

    <p>Changing scrubs daily or whenever they become visibly soiled</p> Signup and view all the answers

    What is the purpose of wearing dedicated non-slip enclosed footwear in CSSD?

    <p>Protecting against injury or contact with sharp objects</p> Signup and view all the answers

    Why should gloves be removed without contaminating hands in the Decontamination Zone?

    <p>To avoid contaminating surfaces and pens</p> Signup and view all the answers

    What is a requirement for wearing fluid-resistant masks with visor eye shields in the Decontamination Zone?

    <p>Avoiding touching mask or eye shield with hands while working</p> Signup and view all the answers

    What is recommended when using disposable shoe covers in the Decontamination Zone?

    <p>Replacing them if there is any sign of damage</p> Signup and view all the answers

    What is emphasized for workwear in the IAP or sterile storage zones in CSSD?

    <p>Washing hands before and after using gloves</p> Signup and view all the answers

    What should be done when gloves become moist or visibly soiled while working in the Decontamination Zone?

    <p>Remove them immediately and discard them appropriately</p> Signup and view all the answers

    What is the main purpose of Standard Precautions in healthcare?

    <p>To minimize the risk of infection regardless of the perceived infectious risk</p> Signup and view all the answers

    Why is it important to be familiar with maintenance of personal hygiene appropriate to the unit's zone in the CSSD?

    <p>To protect reusable medical devices from contamination by personnel</p> Signup and view all the answers

    What is the rationale for applying Standard Precautions in the decontamination zone of the CSSD?

    <p>To handle reusable medical devices safely after decontamination</p> Signup and view all the answers

    What is the main concern addressed by the Chain of Infection concept?

    <p>Transmission of microbial infections and means to prevent it</p> Signup and view all the answers

    Why are hospital patients particularly susceptible to infection?

    <p>Due to being in poor health and having compromised immune systems</p> Signup and view all the answers

    What is an important measure to minimize the reservoir of infection in a hospital environment?

    <p>Thoroughly cleaning and disinfecting the environment and equipment</p> Signup and view all the answers

    Why do hospital staff constantly try to minimize the reservoir of infection in healthcare environments?

    <p>To reduce the spread of infectious microbes around the environment</p> Signup and view all the answers

    What are some examples of infections that have developed resistance in recent times?

    <p>&quot;Methicillin Resistant Staphylococcus aureus (MRSA), multi-drug resistant Tubercle bacillus (MDR TB)&quot;</p> Signup and view all the answers

    What is a key reason for trying to break the chain of infection from various sources or reservoirs in healthcare settings?

    <p>&quot;To prevent infected reusable medical devices from being used on patients&quot;</p> Signup and view all the answers

    Why do healthcare professionals apply prompt treatment measures?

    <p>&quot;To prevent infections from spreading within the healthcare facility&quot;</p> Signup and view all the answers

    What is the main function of the slime matrix secreted by microbes in a biofilm?

    <p>To provide a protective covering for the microbes</p> Signup and view all the answers

    In what way do biofilms make pathogens more difficult to eradicate?

    <p>By providing a protective environment for pathogens to multiply</p> Signup and view all the answers

    Why is it best practice to avoid delays before starting decontamination of used reusable medical devices?

    <p>To reduce the risk of microbial spread to processed devices and staff</p> Signup and view all the answers

    Which type of microorganisms have life-cycle stages that are encysted (within a tough protective capsule)?

    <p>Protozoa</p> Signup and view all the answers

    What characteristic makes fungi resistant to disinfectants?

    <p>Thick cell walls and spores</p> Signup and view all the answers

    How are yeasts identified in the laboratory?

    <p>By culture in the laboratory to form colonies and examining cell shapes under the microscope</p> Signup and view all the answers

    What characteristic distinguishes bacteria from other microorganisms?

    <p>Presence of a nucleus and multiplication by budding</p> Signup and view all the answers

    What is the main challenge in removing bacteria from uneven surfaces during cleaning?

    <p>Resistance of bacteria to heat and chemicals</p> Signup and view all the answers

    What is the main purpose of regular microbiological testing in the CSSD?

    <p>To monitor the efficacy of cleaning and decontamination protocols</p> Signup and view all the answers

    What is the role of neutral detergent and cleaning enzymes when used on reusable medical devices?

    <p>To prevent biofilm formation on the devices</p> Signup and view all the answers

    What is the main method used for identification of fungal pathogens?

    <p>Growth in the laboratory to form colonies</p> Signup and view all the answers

    What is the main reason why spores often resist disinfectants?

    <p>They have a protective outer layer</p> Signup and view all the answers

    Which method is most commonly used to identify bacteria in the laboratory?

    <p>Microscopy of gram stained smears</p> Signup and view all the answers

    How are viruses detected by damage they cause to healthy human or animal cells in culture?

    <p>By forming plaques or areas of destruction</p> Signup and view all the answers

    What is the main reason why prions are difficult to remove from reusable medical devices?

    <p>They are very small and adhere strongly to surfaces</p> Signup and view all the answers

    What is the main function of the commensal microbes found in the human body?

    <p>To stimulate the immune system and carry out beneficial functions</p> Signup and view all the answers

    Which method is usually used to detect prions on reusable medical devices?

    <p>Specific antibodies linked to dyes</p> Signup and view all the answers

    What is the most common method for identifying viruses in the laboratory?

    <p>Tissue culture methods</p> Signup and view all the answers

    What differentiates prions from viruses and bacteria?

    <p>They are proteinaceous units causing TSEs</p> Signup and view all the answers

    What makes microbes difficult to eliminate in clinical environments?

    <p>The potential beneficial functions for humans</p> Signup and view all the answers

    What is the main reason why viruses can be difficult to remove during cleaning?

    <p>They can be resistant to disinfectants and heat</p> Signup and view all the answers

    What is the most usual way that prions are detected?

    <p>By histopathology of infected tissue using specific antibodies linked to dyes</p> Signup and view all the answers

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