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Questions and Answers
Bacillus species are anaerobic, gram-negative rods.
Bacillus species are anaerobic, gram-negative rods.
False (B)
Most members of the Bacillus genus are saprophytic organisms.
Most members of the Bacillus genus are saprophytic organisms.
True (A)
What is the name of the insect pathogen found in soil, water, and air that can also cause disease in humans?
What is the name of the insect pathogen found in soil, water, and air that can also cause disease in humans?
B. Thuringiensis
What are the two forms of food poisoning caused by B cereus?
What are the two forms of food poisoning caused by B cereus?
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What is the incubation period for the diarrheal form of B cereus food poisoning?
What is the incubation period for the diarrheal form of B cereus food poisoning?
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B cereus is resistant to a variety of antimicrobial agents, including penicillins and cephalosporins.
B cereus is resistant to a variety of antimicrobial agents, including penicillins and cephalosporins.
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Anthrax is primarily a disease of herbivores.
Anthrax is primarily a disease of herbivores.
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Humans can become infected with anthrax by inhaling spores.
Humans can become infected with anthrax by inhaling spores.
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Anthrax toxins are made up of four proteins.
Anthrax toxins are made up of four proteins.
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Flashcards
Bacillus
Bacillus
A genus of bacteria that includes large, aerobic, gram-positive rods that occur in chains.
Virulence Plasmids
Virulence Plasmids
A virulence factor that allows certain Bacillus species to cause disease.
Bacillus subtilis
Bacillus subtilis
A Bacillus species that is commonly found in soil, water, and air.
Bacillus thuringiensis
Bacillus thuringiensis
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Bacillus cereus
Bacillus cereus
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Bacillus anthracis
Bacillus anthracis
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Anthrax Spores
Anthrax Spores
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Autoclaving
Autoclaving
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Anthrax
Anthrax
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Anthrax transmission
Anthrax transmission
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Portal of entry for anthrax
Portal of entry for anthrax
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Spore germination in anthrax
Spore germination in anthrax
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Gelatinous edema and congestion in anthrax
Gelatinous edema and congestion in anthrax
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Bacilli spread via lymphatics
Bacilli spread via lymphatics
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Non-virulent Bacillus anthracis
Non-virulent Bacillus anthracis
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Poly-α-d-glutamic acid capsule
Poly-α-d-glutamic acid capsule
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Anthrax toxins
Anthrax toxins
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Protective Antigen (PA)
Protective Antigen (PA)
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Edema Factor (EF)
Edema Factor (EF)
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Lethal Factor (LF)
Lethal Factor (LF)
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Active Immunity to Anthrax
Active Immunity to Anthrax
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Live attenuated Bacillus anthrax vaccine
Live attenuated Bacillus anthrax vaccine
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Spore suspension anthrax vaccine
Spore suspension anthrax vaccine
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PA from culture filtrates anthrax vaccine
PA from culture filtrates anthrax vaccine
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Recombinant PA (rPA) anthrax vaccine
Recombinant PA (rPA) anthrax vaccine
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Antibiotic treatment for anthrax
Antibiotic treatment for anthrax
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Ciprofloxacin
Ciprofloxacin
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Prophylaxis for Anthrax
Prophylaxis for Anthrax
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Emetic type food poisoning
Emetic type food poisoning
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Diarrheal type food poisoning
Diarrheal type food poisoning
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Emetic toxin
Emetic toxin
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Study Notes
Bacillus Species
- Bacillus includes large, aerobic, gram-positive rods, often in chains
- Members are closely related but differ in pathogenicity
- Some species are saprophytes found in soil, water, air, and vegetation (e.g., Bacillus subtilis)
- Pathogenic species have virulence plasmids
- Some cause disease in humans (e.g., B. cereus)
- B. cereus can cause food poisoning, producing toxins that lead to diarrhea or vomiting
- B. cereus and B. thuringiensis can cause disease in immunocompromised individuals
- B. anthracis is a significant pathogen, causing anthrax
- B. anthracis spores reside in the center of the bacilli; they're highly resistant to environmental factors (e.g., heat, chemicals), and can survive for years in dry earth
- Anthrax is primarily a disease of herbivores; other animals like rats are less susceptible
- Humans can be infected by contact with infected animals or their products
Bacillus anthracis
- Portal of entry in animals is the mouth and gastrointestinal tract
- Spores can easily be ingested with spiny or irritating vegetation
- Anthrax is acquired in humans through breaks in skin (cutaneous), mucous membranes (gastrointestinal), or by inhaling spores (inhalation)
- Spores germinate in the infected area and growth causes edema and congestion
- B. anthracis spreads via lymphatic system to the bloodstream, and then tissues
- Non-capsulated B. anthracis strains are not virulent
- The capsule of anthrax is made of poly-a-d-glutamic acid and prevents phagocytosis
- Anthrax toxin consists of three proteins: protective antigen (PA), edema factor (EF), and lethal factor (LF)
Anthrax Toxin
- PA binds to specific cell receptors
- After activation, PA forms a channel, allowing EF and LF to enter the cell
- EF acts as adenylate cyclase, leading to edema formation
- LF plus PA combine to form lethal toxin, a major virulence factor and cause of death
Anthrax resistance and Immunity
- Active immunity in susceptible animals can be developed via vaccination
- Vaccination using live attenuated bacilli, spore suspensions or PA from culture filtrates can induce immunity
Bacillus cereus
- Food poisoning due to Bacillus cereus occurs in two forms: emetic and diarrheal type
- Emetic type is associated with rice, milk, and pasta.
- Diarrheal type is associated with meat and/or sauces.
- Food borne toxin production rather than an infection
- Emetic form causes nausea, vomiting, abdominal cramps, within 1-5 hours post ingestion
- Diarrheal form causes diarrhea and related symptoms within 6-15 hours post ingestion
- B. cereus is resistant to certain antibiotics (e.g., penicillin and cephalosporins)
- Serious non-food borne infections are treated with vancomycin or clindamycin, with or without an aminoglycoside; and ciprofloxacin can be helpful for wound infections
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Description
Explore the characteristics and pathogenicity of Bacillus species in this quiz. Learn about saprophytes, pathogenic members like Bacillus anthracis, and their roles in human diseases. Test your knowledge on food poisoning and the nature of anthrax.