Bacteriology: Corynebacterium and Bacillus Species
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Questions and Answers

Which organism is associated with producing the diphtheria toxin?

  • Corynebacterium diphtheriae (correct)
  • Klebs Loeffler’s Bacillus
  • Staphylococcus aureus
  • Bacillus cereus

What is the primary mode of transmission for the organism responsible for diphtheria?

  • Airborne transmission
  • Foodborne transmission
  • Person to person exposure (correct)
  • Vector-borne transmission

Which of the following characteristics describes Bacillus cereus?

  • Causes fried rice poisoning. (correct)
  • Commonly found in the human nasopharynx.
  • Produces beta-lactamase.
  • Is strictly anaerobic.

What is a notable resistance feature of Bacillus cereus spores?

<p>Resist pasteurization. (B)</p> Signup and view all the answers

Which of the following symptoms is nonspecific for infections caused by Corynebacterium diphtheriae?

<p>Low-grade fever (A)</p> Signup and view all the answers

Which Corynebacterium species is most commonly associated with prosthetic joint infections?

<p>C. jeikeium (D)</p> Signup and view all the answers

What type of infection is C. urealyticum primarily associated with?

<p>Urinary tract infection (D)</p> Signup and view all the answers

Which Corynebacterium species is known to produce a diphtheria-like toxin?

<p>C. ulcerans (A)</p> Signup and view all the answers

Which test is associated with the identification of Corynebacterium species?

<p>Methylene blue stain (D)</p> Signup and view all the answers

What is a common consequence of C. pseudotuberculosis infection in humans?

<p>Lymphadenitis (B)</p> Signup and view all the answers

Which Corynebacterium species is part of the normal flora of the skin?

<p>C. xerosis (B)</p> Signup and view all the answers

C. jeikeium is primarily associated with which type of infections?

<p>Hospital-acquired infections (D)</p> Signup and view all the answers

C. ulcerans is most commonly associated with infections in which animal?

<p>Cattle (A)</p> Signup and view all the answers

What are common symptoms associated with respiratory diphtheria?

<p>Respiratory obstruction and cough (A)</p> Signup and view all the answers

Which two drugs are typically used in the treatment of infections caused by diphtheria?

<p>Penicillin and Erythromycin (B)</p> Signup and view all the answers

Which of the following accurately describes the cutaneous form of diphtheria?

<p>Involves nonhealing skin ulcers (A)</p> Signup and view all the answers

What is the primary site of infection for acute diphtheria?

<p>Tonsils and pharynx (B)</p> Signup and view all the answers

How long is the typical incubation period for the diarrhea-causing form of food poisoning mentioned?

<p>8-16 hours (A)</p> Signup and view all the answers

Which of the following forms of food poisoning typically results in more vomiting?

<p>Emetic food poisoning (D)</p> Signup and view all the answers

What characteristic is used in the laboratory diagnosis of diphtheria?

<p>Presence of Babes-Ernst granules (B)</p> Signup and view all the answers

Which type of complications are considered less common in the context of food poisoning?

<p>Systemic complications (D)</p> Signup and view all the answers

What is the Gram stain result typically seen in diphtheria infections?

<p>Gram-positive pleomorphic bacilli (C)</p> Signup and view all the answers

What is the most serious potential outcome associated with untreated respiratory diphtheria?

<p>Cardiac failure (C)</p> Signup and view all the answers

What is the appearance of Nocardia species on Sabouraud's Dextrose Agar?

<p>Bumpy, velvety, yellow-orange colonies (D)</p> Signup and view all the answers

Which type of infection is primarily caused by Nocardia brasiliensis?

<p>Chronic granulomatous infection of the skin (B)</p> Signup and view all the answers

What characteristic allows Nocardia to be identified under the microscope?

<p>Filamentous branching and partially acid-fast properties (B)</p> Signup and view all the answers

Which culture conditions are necessary for growing Nocardia species?

<p>Common nonselective medium at 22C to 37C (C)</p> Signup and view all the answers

What is the treatment of choice for Nocardia infections due to its resistance profile?

<p>Sulfonamides (B)</p> Signup and view all the answers

Which of the following is a common clinical manifestation of Nocardia asteroides complex?

<p>Chronic, confluent bronchopneumonia (C)</p> Signup and view all the answers

Which of the following statements about the Gram stain characteristics of Nocardia is true?

<p>Nocardia can be mistaken for Gram-positive cocci (A)</p> Signup and view all the answers

What is a significant culturing challenge for Nocardia species?

<p>They require extended incubation periods. (C)</p> Signup and view all the answers

Which feature is true for Bacillus species?

<p>They can be motile. (D)</p> Signup and view all the answers

What is the causative agent of cutaneous anthrax?

<p>Bacillus anthracis (D)</p> Signup and view all the answers

Which statement about Corynebacterium diphtheriae is accurate?

<p>It appears as a club-shaped organism. (C)</p> Signup and view all the answers

What laboratory method can be used to identify Bacillus anthracis?

<p>Direct Fluorescent Antibody Assay (B)</p> Signup and view all the answers

Which symptom is most commonly associated with inhalational anthrax?

<p>Severe respiratory distress (D)</p> Signup and view all the answers

What type of bacteria are classified as catalase positive, non-branched, and non-spore forming?

<p>Corynebacterium (C)</p> Signup and view all the answers

Which virulence factor does Bacillus anthracis possess?

<p>Glutamic acid capsule (C)</p> Signup and view all the answers

What causes injectional anthrax?

<p>Direct injection of spores into tissues (B)</p> Signup and view all the answers

What type of colony morphology is typically observed with Bacillus anthracis?

<p>Non-hemolytic, gray, and raised colonies (D)</p> Signup and view all the answers

What is a common transmission route for gastrointestinal anthrax?

<p>Ingestion of spores (C)</p> Signup and view all the answers

Which of the following correctly describes the Gram stain characteristics of Bacillus species?

<p>Gram-positive bacilli with square ends (C)</p> Signup and view all the answers

Which species is characterized by a 'medusa head' colony morphology?

<p>Bacillus anthracis (D)</p> Signup and view all the answers

What type of bacteria is Nocardia classified as?

<p>Catalase positive filamentous bacteria (A)</p> Signup and view all the answers

What is the appearance of Corynebacterium under microscopy?

<p>Pleomorphic with club shapes (A)</p> Signup and view all the answers

What is the main virulence factor associated with Listeria monocytogenes?

<p>Listeriolysin O (D)</p> Signup and view all the answers

What specific symptom may a pregnant woman experience due to Listeriosis?

<p>Flu-like illness (C)</p> Signup and view all the answers

Which laboratory method is primarily used to confirm the toxigenicity of Corynebacterium diphtheriae?

<p>Elek Test (D)</p> Signup and view all the answers

What is a key characteristic of Erysipelothrix rhusiopathiae?

<p>Catalase negative (A)</p> Signup and view all the answers

Which clinical infection is associated with Erysipelothrix rhusiopathiae?

<p>Localized skin infection (B)</p> Signup and view all the answers

In which condition might you find a beta-hemolytic pattern in bacterial culture?

<p>Group B Streptococcus infection (A)</p> Signup and view all the answers

Which laboratory culture medium is used for isolating Erysipelothrix rhusiopathiae?

<p>BAP and CAP (A)</p> Signup and view all the answers

What is the method of transmission for Listeria monocytogenes?

<p>Ingestion of contaminated food (C)</p> Signup and view all the answers

What distinguishes Actinomycetes from other bacterial groups?

<p>Branching or partially acid-fast features (D)</p> Signup and view all the answers

Which bacterial species is associated with Whipple disease?

<p>Tropheryma whipplei (B)</p> Signup and view all the answers

What is the characteristic morphology of Listeria monocytogenes under the microscope?

<p>Coccobacilli in clusters (C)</p> Signup and view all the answers

What type of motility is observed in Listeria monocytogenes?

<p>Tumbling motility (D)</p> Signup and view all the answers

What clinical presentation is likely in a newborn infected with Listeria monocytogenes?

<p>Meningitis and sepsis (D)</p> Signup and view all the answers

What is the characteristic growth pattern of Erysipelothrix rhusiopathiae in culture?

<p>Inverted Christmas tree pattern (C)</p> Signup and view all the answers

Flashcards

Bacillus cereus

A bacteria commonly found in soil and water. It can cause food poisoning and opportunistic infections. Pasteurization does not kill its spores.

Corynebacterium diphtheriae

A bacteria responsible for the infectious disease diphtheria. It produces a toxin that can cause serious illness.

Diphtheria toxin

The toxin produced by Corynebacterium diphtheriae. It's a key virulence factor that causes the symptoms of diphtheria.

Bacillus cereus

A bacterial species that is known to cause food poisoning. It's often associated with fried rice, hence its common name - "Fried rice poisoning".

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Mode of Transmission (MOT)

The way a disease spreads from one person to another. For diphtheria, it's usually through respiratory droplets or direct contact with skin lesions.

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Corynebacterium

A type of bacteria commonly found on human skin, often associated with prosthetic joint infections.

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C. jeikeium

A specific species of Corynebacterium known to cause prosthetic valve endocarditis in adults.

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C. ulcerans

A species of Corynebacterium associated with infections in cattle, particularly mastitis (inflammation of the mammary gland).

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C. xerosis

A species of Corynebacterium that can cause opportunistic infections in humans.

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C. urealyticum

A species of Corynebacterium linked to urinary tract infections.

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C. pseudotuberculosis

A species of Corynebacterium associated with lymphadenitis (inflammation of lymph nodes) in humans, often following contact with infected livestock.

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C. diphtheriae

A species of Corynebacterium that can cause diphtheria-like infections in humans.

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Metachromatic stain

A method used to stain Corynebacterium bacteria, often using methylene blue dye.

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Diphtheria

A type of bacterial infection caused by Corynebacterium diphtheriae, characterized by the formation of a thick, gray membrane in the throat, which can obstruct breathing.

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Respiratory Diphtheria

A form of diphtheria affecting the tonsils and pharynx, often causing high fever and a thick membrane in the throat.

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Cutaneous Diphtheria

A form of diphtheria affecting the skin, characterized by non-healing ulcers, especially in diabetic patients.

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Botulism

A type of bacterial infection caused by Clostridium botulinum, characterized by muscle paralysis, often affecting the muscles involved in swallowing and breathing.

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Botulism Food Poisoning

A type of food poisoning caused by Clostridium botulinum, where the toxin enters the body through contaminated food.

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Anthrax

A type of bacterial infection caused by Bacillus anthracis, characterized by characteristic skin lesions, often resembling a painless black ulcer.

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Cutaneous Anthrax

A form of anthrax where the bacteria infect the skin, resulting in a painless black ulcer.

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Inhalation Anthrax

A form of anthrax where the bacteria enter the body through inhalation, causing severe respiratory problems.

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Gastrointestinal Anthrax

A form of anthrax where the bacteria infect the digestive system, often through consuming contaminated meat.

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Aerobic Growth

The ability of a bacterium to survive in the presence of oxygen.

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What are Bacillus species?

Gram-positive, spore-forming bacilli. These bacteria are aerobic or facultative anaerobes, meaning they can grow with or without oxygen. They are also catalase-positive, meaning they produce the enzyme catalase, which breaks down hydrogen peroxide. They are commonly found in the environment as contaminants.

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What is Bacillus anthracis?

A species of Bacillus known for its ability to form spores, causing various illnesses like anthrax. Anthrax is a serious disease that can infect humans through skin contact, inhalation, or ingestion. It's important to note that Bacillus anthracis can form spores that are very resistant to environmental factors and can survive for long periods.

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What is Cutaneous anthrax?

A type of anthrax that affects the skin. The infection usually occurs through direct contact with infected animal products like wool, hair, or hides, and it's also known as Woolsorter's disease or Ragpicker's disease.

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What is Inhalation anthrax?

A type of anthrax that enters the lungs by inhaling spores. This is the most fatal form of anthrax as the inhaled spores can quickly multiply in the lungs, leading to severe respiratory distress.

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What is Gastrointestinal anthrax?

A type of anthrax that affects the gastrointestinal tract. It is usually contracted by ingesting contaminated food containing spores. It is often associated with drug users who inject contaminated drugs.

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What is Injectional anthrax?

A type of anthrax that is caused by the direct injection of spores into tissues. This can occur through contaminated needles or from skin-popping.

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What is the composition of the capsule of Bacillus anthracis?

The capsule of Bacillus anthracis is composed of glutamic acid. It acts as a protective barrier preventing the immune system from effectively attacking the bacteria.

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What are the components of Bacillus anthracis exotoxin?

Bacillus anthracis produces a potent exotoxin with three major components: Protective Antigen (PA), Edema Factor (EF), and Lethal Factor (LF).

  • PA binds to receptors on host cells and facilitates the entry of EF and LF.
  • EF causes edema by raising levels of cyclic AMP (cAMP), leading to fluid accumulation.
  • LF inhibits protein synthesis and causes cell death.
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What are Corynebacterium species?

Gram-positive, non-spore-forming bacilli that are pleomorphic, meaning they appear in various shapes. They are also catalase-positive and commonly found in the environment, often as normal flora in the human body.

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What is Corynebacterium diphtheriae?

A species of Corynebacterium that causes diphtheria. It produces a toxin that can cause a severe respiratory infection characterized by a thick, grey membrane forming in the throat. Diphtheria is a serious disease that can be fatal if untreated.

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What is the typical appearance of Corynebacterium species?

Corynebacterium species are often described as 'diphtheroids' due to their club-shaped or coryneform appearance. This refers to their morphology, which includes club-shaped, and picket fence-like arrangements.

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What is Listeria monocytogenes?

A Gram-positive, facultative anaerobic, non-spore-forming bacterium. It is a common foodborne pathogen that can cause listeriosis, a serious infection that can affect pregnant women, newborns, and people with weakened immune systems.

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What is Erysipelothrix rhusiopathiae?

A Gram-positive, facultative anaerobic bacterium generally found in the environment. It is known for causing Erysipelas, a skin infection that results in a red, raised, and painful rash often found on the face or legs.

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What are Actinomycetes?

A group of filamentous bacteria that are generally aerobic or facultative anaerobic. They are also catalase-positive and can cause opportunistic infections in immunocompromised individuals. Some species are known for causing serious conditions like nocardiosis.

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What are Nocardia species?

A genus of Actinomycetes known for its ability to form branching mycelia (filaments). They are often associated with infections of the lungs, skin, and brain, particularly in individuals with weakened immune systems.

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Nocardia

A type of bacteria that is gram-positive, rod-shaped, and can form branching filaments. It is commonly found in soil, water, and humans.

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Nocardia asteroides complex

A species of Nocardia that can cause pulmonary infections, especially in immunocompromised individuals. It is known for its ability to survive in harsh environments.

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Pulmonary Nocardia Infection

A type of infection caused by Nocardia asteroides complex that primarily affects the lungs. It can be chronic and lead to severe lung damage.

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Nocardia brasiliensis

A species of Nocardia that is a common cause of cutaneous infections, particularly in individuals involved in outdoor activities. It can cause skin lesions, nodules, and abscesses.

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Actinomycotic mycetoma

A type of infection caused by Nocardia brasiliensis characterized by the formation of granules in the skin and subcutaneous tissues. It's often seen in individuals who work with soil or are exposed to the outdoors.

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Acid-fast staining

A laboratory technique used to identify bacteria by their ability to take up a stain, specifically Ziehl-Neelsen stain. This helps differentiate bacteria based on their cell wall structure.

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Sabouraud's Dextrose Agar (SDA) 10% CO2

A special type of media used to grow fungi. It provides the necessary nutrients and environment for fungal growth and identification. The presence of 10% CO2 helps facilitate fungal growth.

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Sulfonamides

A common antibiotic used to treat Nocardia infections. It works by inhibiting the growth of the bacteria, helping to control the infection.

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Psuedodiphtheriticum Colony type

A type of colony morphology observed in Corynebacterium diphtheriae, characterized by a club-shaped appearance and a tendency to arrange themselves in palisades (parallel rows).

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Elek Test

A test used to determine if Corynebacterium diphtheriae produces diphtheria toxin, which is responsible for the severity of the disease.

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Listeria monocytogenes

A gram-positive, facultative anaerobic bacterium that causes Listeriosis, an infection that can be serious in pregnant women, newborns, and immunocompromised individuals.

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Listeriolysin O

A virulence factor produced by Listeria monocytogenes that helps the bacteria spread within the host by breaking down cell membranes.

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Protein p60

A protein produced by Listeria monocytogenes that promotes inflammation and contributes to the virulence of the bacteria.

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Erysipelothrix rhusiopathiae

A gram-positive, non-spore-forming, facultative anaerobic bacterium that can cause infections in animals and humans, particularly those in the meat and poultry processing industry.

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Inverted Christmas Tree Colony Morphology

A characteristic feature of Erysipelothrix rhusiopathiae colonies on agar plates, resembling an inverted Christmas tree or umbrella shape.

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CAMP Test

A test used to differentiate between various bacterial species based on their ability to enhance the hemolytic activity of Staphylococcus aureus.

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Erysipeloid

A skin infection caused by Erysipelothrix rhusiopathiae, often affecting the hands and fingers.

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Actinomycetes

A group of bacteria known for their branching, filamentous growth patterns. They are found in various environments. They may be partially acid-fast.

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Tropheryma whipplei

A species of Actinomycetes responsible for causing Whipple's disease, a rare bacterial infection affecting the digestive and joint systems.

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Nocardia sp.

A genus of Actinomycetes that commonly cause infections in humans, particularly in immunocompromised individuals. They can cause lung infections and skin lesions.

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Nontoxigenic Corynebacterium

A general term used to refer to different species of Corynebacterium that do not produce diphtheria toxin. These species are typically harmless or opportunistic.

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Gravis Colony Morphology

A type of colony morphology observed in Corynebacterium diphtheriae, characterized by a circular shape, smooth surface, and a white or gray color.

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Mitis Colony Morphology

A type of colony morphology observed in Corynebacterium diphtheriae, characterized by smaller, less prominent colonies compared to the gravis type.

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Study Notes

Aerobic Gram-Positive Bacilli

  • Bacillus:

    • Gram-positive: A type of bacteria characterized by its cell wall structure retaining the crystal violet stain in a Gram stain.
    • Aerobic: Requiring oxygen for growth.
    • Spore-forming: Able to produce resilient spores, resistant to adverse conditions.
    • Examples: Bacillus anthracis (anthrax), Bacillus cereus (food poisoning).
    • Methods of Diagnosis: Blood, lung tissue, cerebrospinal fluid (CSF) specimens. Nonhemolytic colonies with gray color and raised structure are visible. Microscopically, the spores appear at the center of the cell, square-ended. Gram staining yields a positive result.
  • Corynebacterium:

    • Gram-positive: A type of bacteria retaining the crystal violet stain in Gram staining.
    • Non-spore forming: Doesn't produce spores.
    • Catalase-positive: Produces the enzyme catalase.
    • Examples: Corynebacterium diphtheriae (diphtheria).
    • Clinical presentation: A sore throat, low-grade fever, malaise. If severe, respiratory distress can arise. Leading to potential complications such as respiratory obstruction and damage to heart, kidneys, and peripheral nerves.
    • Other strains: Appear in varied shapes, including club-shaped and Chinese character patterns.
  • Listeria monocytogenes:

    • Gram-positive: Bacteria appearing as coccobacilli or in chains. Retain the crystal violet stain in Gram staining.
    • Catalase-positive: The presence of catalase enzyme is determined.
    • Non-spore forming: Doesn't produce resilient spores.
    • Motile: Exhibit motility.
    • Clinical infection: Most typically occurs by ingestion of contaminated meat or poultry. Leads to Listeriosis.
    • Susceptibility: Pregnant, immunocompromised individuals, and neonates at increased risk.
    • Symptoms: Flu-like illness, spontaneous abortion, stillbirth in pregnant individuals, meningitis, sepsis in neonates, and central nervous system infection among immunocompromised patients
  • Erysipelothrix rhusiopathiae:

    • Gram-positive: The cell wall structure retains the crystal violet stain in a Gram stain.
    • Non-spore forming: Doesn't produce spores.
    • Catalase-negative: Absence of catalase activity (the enzyme catalase)
    • Clinical presentation: Characterized by localized skin infections. These infections are associated with cuts, scratches, and skin abrasions, in scenarios such as handling meat, poultry, and fish.
    • Diagnosis: Tissue biopsy or aspirate of skin lesions. Appearance of thin, V-shaped gram-positive bacilli are evident in microscopic examination. Growing cultures on blood agar plates or nutrient broth are useful, along with observing the presence of catalase negative cultures and checking for the absence of nitrate and VP reactions.
  • Actinomycetes/Nocardia:

    • Gram-positive: The cell wall structure retains the crystal violet stain in a Gram stain, though some strains demonstrate variable staining qualities.
    • Branching: Characterized by a branching filamentous structure.
    • Partially acid-fast: Exhibit a partial ability to retain a red stain when treated with acid, differing from completely acid-fast bacteria like Mycobacterium.
    • Clinical presentation: Associated with pulmonary and cutaneous infections; may lead to chronic infections in lungs, and skin that are often chronic and granulomatous.
    • Identification: Utilizing nonselective growth mediums at temperatures ranging from 22C to 37C. They are commonly observed in soil and water, causing infections in humans.

Food Poisoning

  • Bacillus cereus: A possible cause of food poisoning and a common cause of diarrhea or vomiting.

Additional Information

  • String of pearls: A characteristic morphology of Corynebacterium species.
  • Medusa head colonies: A descriptive visual for Bacillus anthracis cultures.
  • Catalase test: An important diagnostic tool confirming the presence of the catalase enzyme in bacteria.

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Test your knowledge on the characteristics and infections associated with Corynebacterium and Bacillus species. This quiz covers topics including diphtheria toxin production, transmission modes, and specific species-related infections. Perfect for students and professionals in microbiology and infectious diseases.

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