Podcast
Questions and Answers
What is the minimum temperature setting required for effective autoclaving?
What is the minimum temperature setting required for effective autoclaving?
- 121°C (correct)
- 150°C
- 104°C
- 135°C
Which of the following steps must be performed after the sterilization period is complete?
Which of the following steps must be performed after the sterilization period is complete?
- Ensure the pressure reads 0 before opening the door. (correct)
- Remove containers without gloves.
- Immediately open the autoclave door.
- Load additional media while the autoclave is still running.
What is the recommended duration for setting the autoclave time?
What is the recommended duration for setting the autoclave time?
- 5 minutes
- 20 minutes or longer
- 10 minutes
- 15 minutes or longer (correct)
What type of exhaust rate is suggested during the autoclave process?
What type of exhaust rate is suggested during the autoclave process?
Which action should be avoided while operating the autoclave?
Which action should be avoided while operating the autoclave?
Flashcards
Autoclaving
Autoclaving
The process of using high pressure steam to sterilize materials, killing bacteria and other microorganisms.
Sterilization Time
Sterilization Time
The time it takes for the autoclave to reach 121°C and maintain that temperature for sterilization.
Autoclaving Temperature
Autoclaving Temperature
The temperature at which most microorganisms are killed during autoclaving.
Slow Rate of Exhaust
Slow Rate of Exhaust
Signup and view all the flashcards
Heat-proof Gloves
Heat-proof Gloves
Signup and view all the flashcards
Study Notes
Autoclave Procedure
- Autoclaving sterilizes culture media.
- Load the autoclave with prepared culture media.
- Secure the autoclave door.
- Set the autoclave time for 15 minutes (or longer).
- Select slow exhaust.
- Set the autoclave temperature to 121°C.
- Initiate autoclaving by pressing the start button or turning the knob.
- After sterilization, allow pressure to reach 0.
- Carefully open the door and remove the containers using heat-resistant gloves.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
This quiz covers the essential steps for the autoclaving process, used to sterilize culture media. It includes details on loading, securing, and operating the autoclave, as well as safety measures for handling sterilized containers. Master the art of autoclaving and ensure successful lab practices.
