Podcast
Questions and Answers
Why is moist heat considered effective in killing microbes in an autoclave?
Why is moist heat considered effective in killing microbes in an autoclave?
- Inhibits microbial reproduction through low temperature exposure
- Causes coagulation of proteins leading to irreversible loss of function and activity of microbes (correct)
- Destroys cell membranes through dehydration
- Triggers mutation in microbial DNA leading to cell death
How does high pressure contribute to the process of sterilization in an autoclave?
How does high pressure contribute to the process of sterilization in an autoclave?
- Reduces the need for high temperature by increasing humidity
- Decreases the boiling point of water for faster sterilization
- Promotes chemical reactions to neutralize microbes
- Increases the boiling point of water for achieving higher temperature (correct)
What effect does high pressure have on the penetration of heat into materials inside the autoclave?
What effect does high pressure have on the penetration of heat into materials inside the autoclave?
- Facilitates rapid penetration of heat into deeper parts of the material (correct)
- Prevents penetration of heat into materials
- Causes uneven distribution of heat within the materials
- Slows down the penetration of heat due to increased resistance
What is the primary mechanism by which moist heat kills microbes effectively?
What is the primary mechanism by which moist heat kills microbes effectively?
Describe how laminar flow biological safety cabinets provide protection for workers and prevent contamination in laboratory settings.
Describe how laminar flow biological safety cabinets provide protection for workers and prevent contamination in laboratory settings.
What is the principle on which the autoclave works?
What is the principle on which the autoclave works?
Explain the purpose and function of high-efficiency particulate air (HEPA) filters in laminar flow biological safety cabinets.
Explain the purpose and function of high-efficiency particulate air (HEPA) filters in laminar flow biological safety cabinets.
Explain the significance of using surgical masks and cotton plugs on culture vessels in laboratory settings. How do they contribute to maintaining a sterile environment?
Explain the significance of using surgical masks and cotton plugs on culture vessels in laboratory settings. How do they contribute to maintaining a sterile environment?
In what other industries and settings are laminar flow biological safety cabinets commonly employed, and for what purposes?
In what other industries and settings are laminar flow biological safety cabinets commonly employed, and for what purposes?
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