9 Questions
Why is moist heat considered effective in killing microbes in an autoclave?
Causes coagulation of proteins leading to irreversible loss of function and activity of microbes
How does high pressure contribute to the process of sterilization in an autoclave?
Increases the boiling point of water for achieving higher temperature
What effect does high pressure have on the penetration of heat into materials inside the autoclave?
Facilitates rapid penetration of heat into deeper parts of the material
What is the primary mechanism by which moist heat kills microbes effectively?
Degradation of nucleic acids and denaturation of enzymes
Describe how laminar flow biological safety cabinets provide protection for workers and prevent contamination in laboratory settings.
Laminar flow biological safety cabinets force air through HEPA filters and project a vertical curtain of sterile air across the cabinet opening, protecting workers from microorganisms and preventing contamination of the room during the handling of microorganisms and sterile work within the cabinet.
What is the principle on which the autoclave works?
Moist heat sterilization using steam under pressure
Explain the purpose and function of high-efficiency particulate air (HEPA) filters in laminar flow biological safety cabinets.
HEPA filters in laminar flow biological safety cabinets remove 99.97% of 0.3 μm particles, allowing for the creation of a sterile working environment by filtering out microorganisms and particulate matter from the air.
Explain the significance of using surgical masks and cotton plugs on culture vessels in laboratory settings. How do they contribute to maintaining a sterile environment?
Surgical masks and cotton plugs allow air to enter culture vessels while keeping microorganisms out, contributing to the maintenance of a sterile environment during culturing processes.
In what other industries and settings are laminar flow biological safety cabinets commonly employed, and for what purposes?
Laminar flow biological safety cabinets are also employed in research labs and industries, such as the pharmaceutical industry, when a sterile working surface is needed for conducting assays, preparing media, and examining tissue cultures.
Learn about the working principle of autoclaves, which use steam under pressure for moist heat sterilization. Discover how high pressure increases the boiling point of water to achieve effective sterilization temperatures.
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