Autoclave and Sterilization Principles
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Questions and Answers

Why is moist heat considered effective in killing microbes in an autoclave?

  • Inhibits microbial reproduction through low temperature exposure
  • Causes coagulation of proteins leading to irreversible loss of function and activity of microbes (correct)
  • Destroys cell membranes through dehydration
  • Triggers mutation in microbial DNA leading to cell death

    • How does high pressure contribute to the process of sterilization in an autoclave?

  • Reduces the need for high temperature by increasing humidity
  • Decreases the boiling point of water for faster sterilization
  • Promotes chemical reactions to neutralize microbes
  • Increases the boiling point of water for achieving higher temperature (correct)

    • What effect does high pressure have on the penetration of heat into materials inside the autoclave?

  • Facilitates rapid penetration of heat into deeper parts of the material (correct)
  • Prevents penetration of heat into materials
  • Causes uneven distribution of heat within the materials
  • Slows down the penetration of heat due to increased resistance

    • What is the primary mechanism by which moist heat kills microbes effectively?

    <p>Degradation of nucleic acids and denaturation of enzymes</p> Signup and view all the answers

    Describe how laminar flow biological safety cabinets provide protection for workers and prevent contamination in laboratory settings.

    <p>Laminar flow biological safety cabinets force air through HEPA filters and project a vertical curtain of sterile air across the cabinet opening, protecting workers from microorganisms and preventing contamination of the room during the handling of microorganisms and sterile work within the cabinet.</p> Signup and view all the answers

    What is the principle on which the autoclave works?

    <p>Moist heat sterilization using steam under pressure</p> Signup and view all the answers

    Explain the purpose and function of high-efficiency particulate air (HEPA) filters in laminar flow biological safety cabinets.

    <p>HEPA filters in laminar flow biological safety cabinets remove 99.97% of 0.3 μm particles, allowing for the creation of a sterile working environment by filtering out microorganisms and particulate matter from the air.</p> Signup and view all the answers

    Explain the significance of using surgical masks and cotton plugs on culture vessels in laboratory settings. How do they contribute to maintaining a sterile environment?

    <p>Surgical masks and cotton plugs allow air to enter culture vessels while keeping microorganisms out, contributing to the maintenance of a sterile environment during culturing processes.</p> Signup and view all the answers

    In what other industries and settings are laminar flow biological safety cabinets commonly employed, and for what purposes?

    <p>Laminar flow biological safety cabinets are also employed in research labs and industries, such as the pharmaceutical industry, when a sterile working surface is needed for conducting assays, preparing media, and examining tissue cultures.</p> Signup and view all the answers

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