Assessment of Parasitism in Biocontrol Agents
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Questions and Answers

What is the ideal Carbon-Nitrogen ratio (C:N) for the substrate in biocontrol agent production?

  • 50:1 to 70:1
  • 10:1 to 20:1
  • 40:1 to 60:1
  • 20:1 to 40:1 (correct)
  • Which of the following is not a factor considered to enhance mass production of biocontrol agents?

  • Supplementing other nutrient sources
  • The pH of the substrate
  • Adding chemical pesticides for competition (correct)
  • Providing favorable temperature during incubation
  • What is one of the constraints regarding the handling of biocontrol agents?

  • They can have an indefinite expiration date.
  • They need specific conditions for transportation and storage. (correct)
  • They are not affected by temperature during storage.
  • They require complex chemical treatments.
  • Which application method involves coating the roots of seedlings with an antagonist?

    <p>Seedling dip (A)</p> Signup and view all the answers

    What must be carefully examined and labeled due to the short-term nature of biocontrol agents?

    <p>Expiration date (A)</p> Signup and view all the answers

    What is the purpose of feeding trials in laboratory tests for biological control?

    <p>To determine if a predator or parasitoid will consume the pest (A)</p> Signup and view all the answers

    Which test assesses the development and survival rates of a biocontrol agent?

    <p>Life table studies (B)</p> Signup and view all the answers

    What is the main goal of host range testing in biological control assessments?

    <p>To ensure the biocontrol agent only targets the intended pest (B)</p> Signup and view all the answers

    Why are greenhouse tests important in biological control research?

    <p>They help assess if lab results are replicable in more realistic settings. (A)</p> Signup and view all the answers

    What combination of agents was effective against diseases in greenhouse tests?

    <p>T.harzianum AP-001 and Bacillus subtilis AP-01 (A)</p> Signup and view all the answers

    What characterizes field tests in biological control assessments?

    <p>They include multiple sites to test the agent's effectiveness. (C)</p> Signup and view all the answers

    After releasing a biocontrol agent, what is crucial to evaluate its impact?

    <p>Monitoring population densities over time (A)</p> Signup and view all the answers

    Which statement about Bacillus subtilis AP-01 is accurate based on the assessments?

    <p>Its combination with T.harzianum AP-001 showed superior results. (D)</p> Signup and view all the answers

    What factors determine the efficacy of a biocontrol agent?

    <p>Mechanism of action and plant pathogen sensitivity (B)</p> Signup and view all the answers

    Which test method assesses microbial inhibition capabilities in a laboratory setting?

    <p>Antagonism assay (D)</p> Signup and view all the answers

    What is the primary focus of dual culture tests in laboratory assessments?

    <p>Testing the antagonistic capability of a microorganism (D)</p> Signup and view all the answers

    Under what conditions should microbial behavior affecting biocontrol be studied?

    <p>Natural or mimicked natural conditions (A)</p> Signup and view all the answers

    Which method is NOT considered a laboratory test for establishing efficacy for biological control?

    <p>Field tests (A)</p> Signup and view all the answers

    What type of microorganism was evaluated against Fusarium sp. in dual culture tests?

    <p>Trichoderma spp. (D)</p> Signup and view all the answers

    What type of environmental factors can influence the efficacy of biocontrol agents?

    <p>Biotic and abiotic factors (B)</p> Signup and view all the answers

    What specific effect does Trichoderma spp. have on Fusarium fujikuroi?

    <p>Inhibits growth of Fusarium (A)</p> Signup and view all the answers

    What is the first step in the production sequence of biocontrol agents?

    <p>Discovery and identification of biocontrol agents (A)</p> Signup and view all the answers

    Which of the following is NOT a safety test required for biocontrol agents?

    <p>Cost effectiveness (C)</p> Signup and view all the answers

    During which step is genetic stability of biocontrol agents assessed?

    <p>Test genetic stability (A)</p> Signup and view all the answers

    What is necessary for the viable inocula production of biocontrol agents?

    <p>Inexpensive medium (A)</p> Signup and view all the answers

    Which of the following substrates was mentioned as suitable for mass production of antagonists?

    <p>Barley grains (C)</p> Signup and view all the answers

    What temperature range is optimal for the physical growth conditions during mass production?

    <p>24-29°C (C)</p> Signup and view all the answers

    Which step occurs after testing the stability and shelf-life of the biocontrol agent?

    <p>Return on investment analysis (B)</p> Signup and view all the answers

    What was the primary method used to manage rice blast disease in Cambodia?

    <p>Trichoderma (A)</p> Signup and view all the answers

    What is considered a primary requirement to ensure effective mass production of biocontrol agents?

    <p>Readily available substrates (D)</p> Signup and view all the answers

    What is the scientific name for the striped flea beetle studied in the field evaluations?

    <p>Phyllotreta striolata (D)</p> Signup and view all the answers

    Which crop was involved in the experiments conducted for the reduction of flea beetle populations?

    <p>Chinese white cabbages (B)</p> Signup and view all the answers

    What was the focus of the 10-year study conducted in Nigeria mentioned in the assessment?

    <p>Aflatoxin mitigation (D)</p> Signup and view all the answers

    What type of organism is Trichoderma classified as?

    <p>Fungi (B)</p> Signup and view all the answers

    Which of the following methods is NOT mentioned as a biological control approach?

    <p>Releasing ladybugs (C)</p> Signup and view all the answers

    What was used to measure the effectiveness of biological control against the striped flea beetle?

    <p>Reduction rates of adult populations (A)</p> Signup and view all the answers

    What was the purpose of experimenting with resistant varieties in rice blast management?

    <p>To control disease incidence (C)</p> Signup and view all the answers

    Flashcards

    Feeding trials

    Tests to see if a predator or parasitoid will eat a pest under controlled conditions.

    No-choice test

    A feeding trial where the predator/parasitoid is only offered the pest.

    Choice test

    A feeding trial where the predator/parasitoid is offered the pest and other options.

    Life table studies

    Studies to determine the growth, reproduction, and survival of a biocontrol agent.

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    Host range testing

    Identifying if a biocontrol agent only targets the pest or attacks other organisms.

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    Greenhouse tests

    Semi-field tests with controlled environmental parameters.

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    Field tests

    Replicated experiments in real-world conditions to assess the effectiveness of a biocontrol agent.

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    Monitoring (in field tests)

    Tracking the pest and biocontrol agent population levels post-release.

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    Biocontrol agent efficacy

    A biocontrol agent's effectiveness depends on its mechanism of action, application methods, the target pathogens' sensitivity and plant susceptibility, and environmental conditions.

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    Antagonism assay

    A lab test evaluating the ability of one microorganism to suppress another's growth using antimicrobial compounds.

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    Dual Culture test

    A lab test for measuring antagonistic activity by culturing two microorganisms together.

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    Laboratory tests (biocontrol)

    Initial tests conducted in controlled lab environments to assess biocontrol agent efficacy.

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    Greenhouse tests (biocontrol)

    Intermediate tests that conduct assessments of biocontrol agents in a controlled environment (greenhouses) similar to real-world conditions.

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    Field tests (biocontrol)

    Biocontrol agent effectiveness tests carried out in natural settings to assess efficacy under wider conditions and interactions.

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    Farm tests (biocontrol)

    Tests evaluating biocontrol agent performance in field conditions, specifically relevant to farm applications of the biocontrol agent.

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    Trichoderma sp.

    A type of fungi used in controlling plant pathogens, such as Fusarium sp.

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    Rice blast disease control

    Field tests in Cambodia investigated the effectiveness of Trichoderma in reducing rice blast disease incidence in a susceptible rice variety.

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    Phyllotreta striolata control

    Field experiments assessed the effectiveness of entomopathogenic nematodes in reducing adult populations of the striped flea beetle in cabbage fields.

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    Aflatoxin mitigation

    Farm tests in Nigeria evaluated the effectiveness of biological control methods for reducing aflatoxin levels in crops.

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    Trichoderma

    A fungus used to control plant diseases.

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    Entomopathogenic nematodes

    Nematodes that kill insects.

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    Crop Protection

    Agricultural strategies focused on preventing crop damage.

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    Biological Control

    Using living organisms to control pests and diseases.

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    BCA Substrate C:N ratio

    The ideal carbon-to-nitrogen ratio for a substrate used to grow biocontrol agents (BCAs) is between 20:1 and 40:1.

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    BCA Substrate pH

    The optimal pH range for a substrate supporting the growth of BCAs is 5.5 to 6.5.

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    Incubation for BCAs

    Incubation is the period where BCAs are grown in a controlled environment to multiply before they are applied to plants or soil.

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    BCA Application Methods

    Biocontrol agents (BCAs) can be applied in various ways to plants or soil, including seed treatments, seedling dips, foliar sprays, and soil applications.

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    Biopriming

    Seed biopriming involves treating seeds with biocontrol agents (BCAs) to enhance seed germination and plant health.

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    Biocontrol agent production

    The process of making large quantities of beneficial organisms to control pests.

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    Spores or propagules

    The reproductive units of many biocontrol agents, like bacteria or fungi, used for mass production.

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    Viable inoculum

    A biocontrol agent preparation containing living and active organisms ready for application.

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    Inexpensive media

    Affordable materials used to grow and multiply biocontrol agents.

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    Mass production substrates

    Materials used to grow large amounts of biocontrol agents like wheat, straw, or grain bran.

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    Factors for enhanced production

    Conditions like temperature and moisture that optimize the growth of biocontrol agents.

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    Genetic stability

    The ability of a biocontrol agent to maintain its effectiveness over time.

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    Shelf-life

    The length of time a biocontrol agent remains effective when stored.

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    Study Notes

    Assessment of Parasitism

    • Microbial behavior impacting biocontrol should be studied under natural or mimicking conditions.
    • Biocontrol agent effectiveness depends on:
      • Mechanism of action (conditioning, dosage, application methods)
      • Target pathogen sensitivity
      • Host (cultivar type, physical properties)
      • Environmental conditions (biotic and abiotic factors, chemical residues, nutrient availability, temperature, moisture).

    Assessment Methods for Establishing Efficacy for Biological Control

    • Laboratory tests
    • Greenhouse tests
    • Field tests
    • Farm tests

    Laboratory Tests

    • Antagonism assay: Evaluates one microorganism's ability to inhibit another's growth, through antimicrobial compound production.

    • Dual Culture tests: Assessing antagonistic capability of Trichoderma isolates against F. fujikuroi.

    • Analyzing suppression and inhibition effects (Trichoderma spp. against F. fujikuroi), measured after seven days incubation, at a temperature of 28±2°C.

    • Evaluating the antagonistic activity of Bacillus subtilis HK-CSM-1 on the mycelial growth of Colletotrichum panacicola.

    • Feeding trials: Assess if a predator or parasitoid consumes a pest under controlled conditions.

      • No-choice test
      • Choice test
    • Life table studies: Determine development, reproduction, and survival rates of a biocontrol agent.

      • Includes data on egg, larva, pupa, and adult stages, with mortality factors like predation, parasitization, and freezing.
    • Host range testing: Determine if potential biocontrol agents target only the pest without harming beneficial or non-target species.

    Greenhouse Tests

    • Semi-field conditions, controlled environment more realistic than lab.

    • Select and evaluate the best potential biocontrol agents.

    • Greenhouse evaluation of Bacillus subtilis AP-01 and Trichoderma harzianum AP-001 for tobacco diseases.

      • Combining these agents creates an effective treatment similar to chemical treatments for both bacterial wilt and damping-off disease.
      • This combination similarly controls frogeye leaf spot, yielding comparable results to chemical control.
    • Assessment of Trathala flavoorbitalis as an egg-plant fruit and shoot borer (FSB) parasitoid.

      • High efficiency in greenhouse and microplot tests, with 90% and 70% parasitism rates, respectively.
      • Population increases with suitable conditions, with about three-fold increase in one-year.

    Field Tests

    • Replicated experiments, using multiple sites or plots with biocontrol agents, compared to untreated or differently treated plots.

    • Monitor pest and biocontrol agent population densities over time to gauge impact.

    • Managing rice blast in Cambodian rice fields uses Trichoderma harzianum.

      • Shows it controls blast incidence effectively in susceptible varieties.
    • Field evaluation of entomopathogenic nematodes to control Phyllotreta striolata.

      • Reports reduction rates of adult populations of Phyllotreta striolata after treatment.

    Farm Tests

    • "Ground-truthing" and assessing the effectiveness of biocontrol agents for mitigating aflatoxin in Nigerian farming fields, over a 10-year period.

    Mass Production

    • Substrates: Wheat, chopped straw (with acid mineral solution), grain bran, wheat bran, barley grains, or sand and corn meal.
    • Advantages of Substrates: Readily available, high stored energy, structure to the final product.
    • Physical Growth Conditions needed: Temperature (24-29°C), Moisture (55-65%), Carbon-Nitrogen ratio (20:1 to 40:1), Substrate pH (5.5-6.5) and other required nutrients.

    Application/Field Release

    • Introduction of antagonists intensifies microbial interaction, controlling disease-causing organisms.
    • Seed treatment, seedling dip, seed-biopriming, foliar spray application and soil application/direct application methods.
    • Coating seedling roots with an antagonist is a viable method, suppressing certain diseases.

    Constraints

    • Biocontrol agents need to effectively compete with chemical pesticides.
    • Ensuring high product potency and purity during delivery to users.
    • Handling, transportation and storage requirements must be well-defined for biocontrol agents.
    • Managing short-term expiration dates for biocontrol agents is critical.

    Steps/Sequence for Production of Biocontrol Agents

    • Discovery and identification of agents
    • Efficacy Test
    • Safety Tests (user, environmental & non-target organisms)
    • Testing genetic stability of the agents
    • Evaluating potential for mass production
    • Formulation
    • Testing stability and shelf-life
    • Market potential testing
    • Evaluation and analysis of profitability/Return on investment (ROI)
    • Field trials
    • Patenting the agent
    • Commercialization and distribution to end-users.

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    Related Documents

    Assessment Of Parasitism PDF

    Description

    This quiz explores the various aspects of parasitism and its implications for biological control. It covers the mechanisms of action, factors affecting biocontrol agent effectiveness, and methods of assessment in laboratory, greenhouse, field, and farm settings. Test your knowledge on microbial behavior and interactions with pathogens.

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