AP-HIS-511 IHC ISH Ventana Benchmark Ultra Troubleshooting

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Questions and Answers

What should be done to ensure that specimens adhere to the slide during IHC/ISH?

Use uncharged slides

Use charged slides (correct)

Use a microscope

Use a slide warmer

True or false: Placement of tissue on charged slides can help resolve the "double dipping" issue.

True

What are two common problems that can arise when performing IHC/ISH?

Common problems that can arise when performing IHC/ISH include specimen not adhering to the slide and the antibody/probe dispenser being empty.

The specimen must ____ to the slide in order to be analyzed.

adhere Signup and view all the answers

What should be done if the antibody/probe dispenser on an immunohistochemistry instrument is empty?

Refill the dispenser Signup and view all the answers

Placement of tissue on ____ slides may cause problems.

uncharged Signup and view all the answers

True or false: The control tissue may stain weakly when the antibody/probe dispenser on an immunohistochemistry instrument is empty.

False Signup and view all the answers

How can the issue of “double dipping” be resolved?

The issue of “double dipping” can be resolved by using charged slides. Signup and view all the answers

To resolve the "double dipping" issue, use ____ slides.

charged Signup and view all the answers

True or false: The back-up software should be backed up daily.

False Signup and view all the answers

What could be the cause of a tissue not staining even though the antibody/probe dispenser is full?

The cause of a tissue not staining even though the antibody/probe dispenser is full could be that the instrument part that depresses the dispenser so the antibody/probe dispenses is broken. Signup and view all the answers

What could be the cause of a tissue staining weakly?

The control tissue has lost its antigenicity Signup and view all the answers

What should be done to prevent double dipping?

Use charged slides Signup and view all the answers

True or false: The instrument part that depresses the dispenser so the antibody/probe dispenses may be broken.

True Signup and view all the answers

The control tissue has lost its ____, and the tissue may stain weakly.

antigenicity Signup and view all the answers

What might be the cause of a tissue staining weakly?

The cause of a tissue staining weakly might be that the control tissue has lost its antigenicity. Signup and view all the answers

The instrument should be manually ____ and the back-up software should be weekly backed up.

restarted Signup and view all the answers

What should be done to ensure that the instrument is running properly?

To ensure that the instrument is running properly, it should be manually restarted and the back-up software should be weekly backed up. Signup and view all the answers

True or false: Specimen must adhere to the slide in order for it to be analyzed.

True Signup and view all the answers

What should be done regularly to ensure the instrument functions properly?

Backup the software Signup and view all the answers

Study Notes

The dictation portion of this standard operating procedure (SOP) is applicable to all staff that gross tissue specimens (staff pathologists, resident pathologists and pathologist assistants). The transcription portion of this SOP is applicable to all personnel assigned to the Department of Pathology and Area Laboratory Services (DPALS).
The dictation portion of this SOP is designed to provide an efficient means of dictating surgical reports to furnish accurate and timely transcription of material.
The transcription section provides dictating capability to the Department of Pathology and Area Laboratory Services (DPALS).
All staff must read and adhere to the stated guidelines and procedures as applicable. Personnel assigned will perform daily tasks utilizing the step-by-step instructions delineated in sections VI thru VIII of this SOP.
The responsibility for dictating tissue specimens falls on the staff pathologist. The resident pathologist/staff pathologist for whom the specimen is being dictated is identified in the first batch of dictations. After dictating and sending the last batch of the day to the transcription queue for transcription, the staff pathologist presses the “stop” button to close out for the day.
The transcription section provides dictating capability to the Department of Pathology and Area Laboratory Services (DPALS). The dictation portion of this SOP is applicable to all staff that gross tissue specimens. The transcription section is also applicable to all personnel assigned to the Department of Pathology and Area Laboratory Services (DPALS). All staff must read and adhere to the stated guidelines and procedures as applicable. Personnel assigned will perform daily tasks utilizing the step-by-step instructions delineated in sections VI thru VIII of this SOP.
The patient is Jane Doe, age 30, with last name of Doe and first name of Jane.
The specimen is a white soft tissue fragment that is 0.4 x 0.4 x 0.4 cm in size.
The specimen was received in formalin and is labeled with Doe, Jane, 30/1234.
The gross pathologist is ____________ and the resident pathologist is ____________.
The primary pathologist is ____________.
The gross pathologist is completing the final diagnosis.
The final diagnosis is tissue, site, procedure.
To amend a case, first log on to CoPath and select <E> (Specimen Data Entry/Edit).
Then select <AM> (Amendment) from the Specimen Edit Option menu.
Enter the accession number of the case, and <Return>.
The pathologist has signed out of the case on the specified date. If you want to amend the case, the pathologist will need to be contacted and given the reason for the amendment.
The pathologist will provide a reason for the amendment in the Amendment Option menu.
The pathologist is reviewing a consult that was submitted with the specimen.
The pathologist will enter the diagnosis and comment into the free text field.
The pathologist will enter the pathologist's last name and the pathologist on-call when addendums are entered for pathologists who are on leave/TDY.
The pathologist will enter the report from the hardcopy consult into the transcription software.
The pathologist will enter the spell check report.
The pathologist will print one copy of the report.

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