Bacterial DNA and Gene Cloning Quiz

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Questions and Answers

Which of these is a double-stranded, self-replicating, circular DNA molecule present in bacteria and widely used as a gene cloning vector?

  • Plasmid (correct)
  • Phagemid
  • Genome
  • Cosmid

Which enzymes are called 'molecular scissors' and are essential for making internal cuts in a DNA molecule or vector at specific sites?

  • Polymerase
  • All of these
  • Restriction proteins
  • Restriction enzymes (correct)

What do we call the new molecule after the process of ligation, where the vector plasmid is joined with the gene of interest?

  • All of these
  • Chimeric DNA
  • Gene of interest
  • Recombinant DNA (correct)

During the transfer of the recombinant plasmid into a host cell, commonly a bacterium, the recombinant bacterium will undergo cloning. Which of the following processes is involved in gene cloning?

<p>Replication (A)</p> Signup and view all the answers

Bacterial cells can be used as host organisms to express genes and make protein products such as insulin and growth hormones. Which of the following processes are involved in this?

<p>Transcription and translation (D)</p> Signup and view all the answers

In plant genetic engineering, which of the following acts as a vector?

<p>Agrobacterium tumefaciens (A)</p> Signup and view all the answers

Which of these is a self-replicating, circular DNA molecule present in bacteria and widely used as a gene cloning vector?

<p>Plasmid (A)</p> Signup and view all the answers

Which enzymes are essential for making internal cuts in a DNA molecule or vector at specific sites, and are called 'molecular scissors'?

<p>Restriction enzymes (C)</p> Signup and view all the answers

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