4 Questions
What do restriction endonucleases do in a bacteria's restriction system?
Destroy the incoming DNA if it is recognized as foreign
What is the purpose of a vector in gene cloning?
To transport the gene into a host cell
What is the result of restriction endonucleases cleaving the incoming DNA?
Random synthesis of DNA fragments
What happens when the host cell divides after vector replication?
Copies of the recombinant DNA molecule are passed to the progeny
Study Notes
Restriction System in Bacteria
- Restriction endonucleases defend bacteria against foreign DNA by cutting it into smaller pieces, preventing its replication.
Gene Cloning
- A vector is a small, self-replicating DNA molecule that carries a foreign gene into a host cell, allowing it to replicate and express the gene.
- The purpose of a vector is to act as a carrier of the foreign gene, enabling its introduction and expression in a host cell.
Restriction Endonucleases' Action
- Restriction endonucleases cleave the incoming DNA at specific recognition sites, producing fragments with sticky ends.
Vector Replication and Host Cell Division
- After vector replication, the host cell divides, and the vector is passed to daughter cells, ensuring the continued presence of the foreign gene.
Test your knowledge of gene cloning in the context of recombinant DNA technology. Understand the steps involved in a gene cloning experiment and the role of vectors in transporting genes into host cells.
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