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Questions and Answers
What do restriction endonucleases do in a bacteria's restriction system?
What is the purpose of a vector in gene cloning?
What is the result of restriction endonucleases cleaving the incoming DNA?
What happens when the host cell divides after vector replication?
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Study Notes
Restriction System in Bacteria
- Restriction endonucleases defend bacteria against foreign DNA by cutting it into smaller pieces, preventing its replication.
Gene Cloning
- A vector is a small, self-replicating DNA molecule that carries a foreign gene into a host cell, allowing it to replicate and express the gene.
- The purpose of a vector is to act as a carrier of the foreign gene, enabling its introduction and expression in a host cell.
Restriction Endonucleases' Action
- Restriction endonucleases cleave the incoming DNA at specific recognition sites, producing fragments with sticky ends.
Vector Replication and Host Cell Division
- After vector replication, the host cell divides, and the vector is passed to daughter cells, ensuring the continued presence of the foreign gene.
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Description
Test your knowledge of gene cloning in the context of recombinant DNA technology. Understand the steps involved in a gene cloning experiment and the role of vectors in transporting genes into host cells.