Recombinant DNA Technology: Gene Cloning
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Recombinant DNA Technology: Gene Cloning

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Questions and Answers

What do restriction endonucleases do in a bacteria's restriction system?

Destroy the incoming DNA if it is recognized as foreign

What is the purpose of a vector in gene cloning?

To transport the gene into a host cell

What is the result of restriction endonucleases cleaving the incoming DNA?

Random synthesis of DNA fragments

What happens when the host cell divides after vector replication?

<p>Copies of the recombinant DNA molecule are passed to the progeny</p> Signup and view all the answers

Study Notes

Restriction System in Bacteria

  • Restriction endonucleases defend bacteria against foreign DNA by cutting it into smaller pieces, preventing its replication.

Gene Cloning

  • A vector is a small, self-replicating DNA molecule that carries a foreign gene into a host cell, allowing it to replicate and express the gene.
  • The purpose of a vector is to act as a carrier of the foreign gene, enabling its introduction and expression in a host cell.

Restriction Endonucleases' Action

  • Restriction endonucleases cleave the incoming DNA at specific recognition sites, producing fragments with sticky ends.

Vector Replication and Host Cell Division

  • After vector replication, the host cell divides, and the vector is passed to daughter cells, ensuring the continued presence of the foreign gene.

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Description

Test your knowledge of gene cloning in the context of recombinant DNA technology. Understand the steps involved in a gene cloning experiment and the role of vectors in transporting genes into host cells.

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