Podcast
Questions and Answers
⭐️What is the primary type of microbial community associated with apical periodontitis?
⭐️What is the primary type of microbial community associated with apical periodontitis?
⭐️ Which bacterial morphotypes are commonly found in the root canal microbiota during primary infections?
⭐️ Which bacterial morphotypes are commonly found in the root canal microbiota during primary infections?
Which part of the tooth is LEAST likely to be penetrated by bacterial cells in apical periodontitis?
Which part of the tooth is LEAST likely to be penetrated by bacterial cells in apical periodontitis?
⭐️What percentage of teeth showing apical periodontitis lesions is likely to have dentinal tubule infection?
⭐️What percentage of teeth showing apical periodontitis lesions is likely to have dentinal tubule infection?
Signup and view all the answers
What is a likely source of nutrients for bacterial cells within dentinal tubules during infection?
What is a likely source of nutrients for bacterial cells within dentinal tubules during infection?
Signup and view all the answers
⭐️What is true about bacteria within Dentin tubules?
⭐️What is true about bacteria within Dentin tubules?
Signup and view all the answers
Which of the following bacterial genera did NOT show penetration into dentinal tubules according to in vitro studies?
Which of the following bacterial genera did NOT show penetration into dentinal tubules according to in vitro studies?
Signup and view all the answers
⭐️What is the most likely cause for nutrient availability for bacterial cells within dentinal tubules?
⭐️What is the most likely cause for nutrient availability for bacterial cells within dentinal tubules?
Signup and view all the answers
Which bacterial morphotype is not commonly associated with primary infections of apical periodontitis?
Which bacterial morphotype is not commonly associated with primary infections of apical periodontitis?
Signup and view all the answers
⭐️What is the depth that bacterial cells can reach into dentinal tubules during an infection?
⭐️What is the depth that bacterial cells can reach into dentinal tubules during an infection?
Signup and view all the answers
Which technique was used to observe the occurrence of specific bacteria inside dentinal tubules?
Which technique was used to observe the occurrence of specific bacteria inside dentinal tubules?
Signup and view all the answers
⭐️Which of the following bacteria has been identified as a common isolate at varying depths within the root dentin?
⭐️Which of the following bacteria has been identified as a common isolate at varying depths within the root dentin?
Signup and view all the answers
Study Notes
Apical Periodontitis and Biofilms
- Apical periodontitis, similar to caries and periodontal diseases, is a biofilm-related disease.
- The root canal microbiome in primary infections is diverse with cocci, rods, filaments, and spirilla (spirochetes) as dominant morphotypes.
- Fungi are occasionally found in root canals.
- The majority of root canal bacteria form multispecies biofilms attached to the dentinal walls.
- Biofilms can also be found in lateral canals, apical ramifications, and isthmuses connecting main canals.
- Bacterial cells within biofilms often penetrate dentinal tubules.
- Dentinal tubule infection is present in a significant majority (70-80%) of teeth with apical periodontitis lesions.
- Bacterial cells can penetrate up to 300 micrometers deep into the dentinal tubules.
- Dividing bacterial cells within tubules indicate active growth and nutrient acquisition from degraded odontoblastic processes, denatured collagen, dead bacterial cells, and fluids entering the tubules.
Endodontic Microbiota Distribution
- Endodontic infections, like caries and periodontal diseases, are biofilm-related.
- The main root canal microbiota consists of a variety of bacterial morphotypes, including cocci, rods, filaments, and spirilla (spirochetes).
- Fungal cells are occasionally found in the root canal system.
- Most bacteria colonize the root canal system in sessile multispecies biofilms attached to the dentinal walls.
- Biofilms can also clog lateral canals, apical ramifications, and isthmuses connecting main canals.
- Bacterial cells in the endodontic biofilm structure often penetrate dentinal tubules.
- Dentinal tubule infection is common in teeth with apical periodontitis lesions (70-80%).
- Bacterial cells can penetrate dentinal tubules up to 300 µm deep.
- Dividing bacterial cells within tubules indicate they are alive and likely obtain nutrients from degrading odontoblastic processes, denatured collagen, dead bacteria, and intracanal fluids.
- Several putative endodontic pathogens have been shown to penetrate dentinal tubules in vitro, including Porphyromonas endodontalis, Porphyromonas gingivalis, Fusobacterium nucleatum, Actinomyces israelii, Propionibacterium acnes, Enterococcus faecalis, Candida albicans, and streptococci.
- A clinical study identified bacteria in root dentin, with the most common isolates being Prevotella, Porphyromonas, Fusobacterium, Veillonella, Peptostreptococcus, Eubacterium, Actinomyces, lactobacilli, and streptococci.
- Immunohistologic analysis found F. nucleatum, Pseudoramibacter alactolyticus, Eubacterium nodatum, Lactobacillus casei, and Parvimonas micra inside dentinal tubules from extracted teeth with apical periodontitis.
- Planktonic bacteria in the main root canal are more easily accessed and eliminated during endodontic treatment compared to bacteria within biofilms, isthmuses, recesses, lateral canals, and dentinal tubules.
- Bacteria in these difficult-to-reach locations may require specialized therapeutic strategies for eradication.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
