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Questions and Answers
What is required for the formation of a visible precipitate in antigen-antibody reactions?
What is required for the formation of a visible precipitate in antigen-antibody reactions?
- Monovalent antibody and bivalent or polyvalent antigen
- Monovalent antibody and monovalent antigen
- Bivalent antibody and bivalent or polyvalent antigen (correct)
- Bivalent antibody and monovalent antigen
What are antibodies called when they aggregate soluble antigens?
What are antibodies called when they aggregate soluble antigens?
- Agglutinins
- Opsonins
- Precipitins (correct)
- Neutralizing antibodies
What is the purpose of centrifuging the tubes in a quantitative precipitation reaction?
What is the purpose of centrifuging the tubes in a quantitative precipitation reaction?
- To separate the antibody and antigen
- To pellet the precipitate (correct)
- To mix the antibody and antigen
- To measure the amount of antigen
What does a precipitin curve measure?
What does a precipitin curve measure?
What type of antigen is required for the formation of an Ag-Ab lattice?
What type of antigen is required for the formation of an Ag-Ab lattice?
Flashcards
Precipitins
Precipitins
Antibodies that cause soluble antigens to clump together.
Bivalent antibody
Bivalent antibody
An antibody that binds to two antigen sites at the same time, promoting cross-linking and precipitation.
Bivalent or Polyvalent Antigen
Bivalent or Polyvalent Antigen
An antigen that can bind to multiple antibody molecules at the same time, forming a large, insoluble complex.
Centrifugation
Centrifugation
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Precipitin curve
Precipitin curve
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Study Notes
Antigen-Antibody Reactions
- A visible precipitate forms when antigen-antibody complexes aggregate to a size that can be seen, requiring a sufficient concentration of both antigen and antibody.
- Antibodies that aggregate soluble antigens are called precipitins.
Quantitative Precipitation Reaction
- Centrifuging the tubes in a quantitative precipitation reaction separates the precipitate from the supernatant, allowing for measurement of the precipitate.
Precipitin Curve
- A precipitin curve measures the amount of precipitate formed as a function of antigen concentration, providing a quantitative representation of the antigen-antibody reaction.
Antigen Requirements
- For the formation of an Ag-Ab lattice, a multivalent antigen (having multiple epitopes) is required, allowing for the cross-linking of antibodies and subsequent precipitation.
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