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Questions and Answers
What happens when too little EDTA is used during blood collection?
What is a consequence of using excess EDTA in blood samples?
Which factor is NOT affected by excess EDTA in a blood sample?
What precaution should be taken during the preparation of anticoagulants?
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What is one advantage of using EDTA as an anticoagulant?
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What is the primary role of EDTA in laboratory settings?
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Which form of EDTA is specifically recommended for use as an anticoagulant?
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What is the concentration of EDTA used per ml of blood in the preparation?
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How is the EDTA solution prepared before storing it in vails?
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What is the total mass of EDTA powder used per vail after preparation?
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What role does heating serve during the preparation of the EDTA solution?
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At what temperature are the vails containing EDTA solution stored in the hot air oven?
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What measurement technique ensures accurate weighing of EDTA powder?
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Study Notes
Anticoagulant Vail Preparation (EDTA)
- Aim: To prepare EDTA vails for the laboratory to prevent blood clotting.
- EDTA (ethylenediaminetetraacetic acid) is a chelating agent that binds to calcium ions, which are essential for blood clotting.
- EDTA is commonly used as an anticoagulant in hematology laboratories, preserving cell morphology.
- A 10% w/v EDTA solution is prepared using 10 mg of EDTA powder and 100 ml of distilled water.
- 40 µl of the EDTA solution is pipetted into each clean vail, containing 4 mg of dry chemical.
- The vails with EDTA solution are placed in a hot air oven at 40 degrees Celsius for 3-4 hours to dry, ensuring the EDTA powder remains at the bottom of the vail.
- Once dried, the vails are closed with a lid and stored in a safe place with labeling.
- Each vail can hold 2.5 ml of blood.
Precautions
- Inadequate EDTA: May lead to blood coagulation.
- Excessive EDTA: May cause cell shrinkage, morphological changes, and artificially high platelet counts.
- Incorrect Order of Draw: Following the correct order of draw for blood collection tubes is crucial to prevent contamination and ensure accurate test results.
Understanding Anticoagulants
- Mechanism of Action: Anticoagulants work by preventing the formation of blood clots by interfering with various clotting factors.
- EDTA is a chelating agent that binds calcium ions, essential for blood clotting, preventing clot formation.
- Concentration of Anticoagulants: The concentration of EDTA is crucial for its effectiveness. Too little EDTA may not prevent clotting, while too much EDTA may affect blood cell morphology and analysis.
- Order of Draw: A specific order of draw is used for venipuncture to minimize the risk of contamination between tubes containing different anticoagulants and additives.
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Description
This quiz covers the preparation of EDTA vails used in laboratories to prevent blood clotting. It explains the role of EDTA as a chelating agent, the preparation process, and precautions to take during the procedure. Understanding these concepts is essential for ensuring accurate results in hematology.
