Podcast
Questions and Answers
What is the purpose of using antiglycolytic agents in the laboratory?
What is the purpose of using antiglycolytic agents in the laboratory?
Which of the following chemicals is commonly used as an anticoagulant in blood collection tubes?
Which of the following chemicals is commonly used as an anticoagulant in blood collection tubes?
Why are color additives included in some blood collection tubes?
Why are color additives included in some blood collection tubes?
How do heparin-coated tubes prevent clotting of blood samples?
How do heparin-coated tubes prevent clotting of blood samples?
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In the context of blood collection, what is the role of citrate as an additive?
In the context of blood collection, what is the role of citrate as an additive?
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Which of the following best describes the function of EDTA as an anticoagulant?
Which of the following best describes the function of EDTA as an anticoagulant?
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What is the purpose of using heparin in blood collection tubes?
What is the purpose of using heparin in blood collection tubes?
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Why is it important to ensure accurate labeling of tubes containing EDTA?
Why is it important to ensure accurate labeling of tubes containing EDTA?
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What role does EDTA play when added to blood samples?
What role does EDTA play when added to blood samples?
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Why are heparin-coated tubes preferred for certain blood tests?
Why are heparin-coated tubes preferred for certain blood tests?
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Study Notes
Blood Collection Tubes
- Versene: disodium salt of EDTA, prevents clotting by chelating calcium phosphate, and stabilizes pH
- Sequestrene: di-potassium salt of EDTA, has better stability than versene, and prevents clotting by chelating calcium
- K2 EDTA: dipotassium salt of EDTA, used to prevent coagulation by binding calcium
- K3 EDTA: tripotassium salt of EDTA, used to prevent coagulation by binding calcium
- Citrate: prevents clotting by binding calcium, and is used in blood transfusions to preserve RBCs
- EDTA: chelates calcium, preventing clotting
- Sodium Fluoride: antiglycolytic agent, prevents glycolysis
Additives and Anticoagulants
- Anticoagulants: prevent clotting by chelating or precipitating calcium
- EDTA: chelates calcium, preventing clotting
- Citrate: binds calcium, preventing clotting
- Oxalate: precipitates calcium, preventing clotting
- Heparin: prevents clotting by inhibiting thrombin formation
- Potassium Oxalate: most widely used anticoagulant, precipitates calcium
- Sodium Polyanethol Sulfonate (SPS): prevents coagulation by binding calcium
Phlebotomy Equipment
- Evacuated Tube System (ETS): closed system for collecting blood
- Multisample needles: allow multiple tubes to be collected with one needle stick
- Hypodermic needles: used for blood collection
- Winged infusion set (butterfly needles): used for blood collection
- Tourniquet: used to restrict blood flow to the vein
- Gloves and glove liners: used for personal protection
- Antiseptics: substances used to prevent sepsis and inhibit microorganisms
- Disinfectants: chemical solutions used to remove or kill microorganisms on surfaces
Laboratory Safety and Personal Hygiene
- Biological hazards: potentially harmful microorganisms
- Chemical hazards: chemicals that can cause harm
- Flammables: substances that can ignite and cause a fire
- Pyrophoric: substances that ignite spontaneously in air
- Self-Reactive: substances that can ignite or explode
- Organic peroxides: substances that can ignite or explode
- Irritant: substances that can cause skin or eye irritation
- Skin sensitizer: substances that can cause skin irritation
- Acute toxicity: substances that can cause harm
- Narcotic effects: substances that can cause drowsiness or disorientation
Chemical Spill Management and Waste Disposal
- Chemical spill management: procedures for cleaning up chemical spills
- Chemical waste disposal: procedures for disposing of chemical waste
- Material Safety Data Sheets (MSDS): documents that provide information on chemical hazards
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Description
Explore the differences between Versene and Sequestrene, two types of EDTA and citrate salts used in blood transfusion. Learn how they prevent clotting, stabilize pH, and preserve blood cells for coagulation tests.
