EDTA Anticoagulant Preparation Quiz

What is the primary function of EDTA in laboratory settings?

What is the correct concentration of dipotassium EDTA recommended for anticoagulation?

How much EDTA solution is pipetted into each vail?

What temperature is recommended for drying the vails in the hot air oven?

What are the primary ions that EDTA binds to?

What is the weight of EDTA powder needed for making the solution?

Which of the following is NOT mentioned as a requirement for the preparation of the anticoagulant vail?

What is the resulting amount of EDTA powder per vail after pipetting 40 µl?

EDTA Anticoagulant Preparation

EDTA (Ethylenediaminetetraacetic acid) is used as anticoagulant in laboratories, especially hematology.
EDTA is a powerful calcium chelator (binding agent) that preserves cell morphology.
The recommended EDTA concentration is 1.5 (±) 0.25 mg/ml of blood.
A 10% w/v EDTA solution is prepared.
40 µl of the 10% EDTA solution is pipetted into each vial, containing 4 mg of dry EDTA powder.
This amount of EDTA is sufficient to prevent coagulation of a 2.5 ml blood sample.
The EDTA solution is heated in a hot air oven at 40°C for 3-4 hours to evaporate the water, leaving the EDTA powder at the bottom of the vial.
The vials are then closed with a lid and stored in a safe place, labeled for future use.

Procedure Summary

The procedure involves preparing a 10% (w/v) EDTA solution by dissolving 10g of EDTA powder in 100ml of distilled water.
40 µl of this solution is then pipetted into each vial, containing 4 mg of dry EDTA powder.
The vials are then heated in a hot air oven to evaporate the water, leaving the EDTA powder at the bottom of the vial.
The vials are then closed with a lid and stored in a safe place, labeled for future use.

Calculation

The concentration of EDTA used is 10 g/100 ml, which is equal to 100 g/L, 100 mg/ml, and 100 µg/µl.
40 µl of the solution contains 4000 µg of EDTA, which equates to 4 mg of EDTA powder per vial.

This quiz covers the preparation and use of EDTA as an anticoagulant in hematology laboratories. You will learn about the correct concentrations, procedures for preparation, and the significance of EDTA in preserving cell morphology. Test your knowledge on this essential laboratory technique!