Antibiotic Selection in Bacterial Transformation

It indicates that they have taken up recombinant plasmids.

Because people living with diabetes required it to be administered artificially into their body.

It consists of two polypeptide chains known as the alpha and beta subunits.

<p>Because insulin consists of two polypeptide chains (alpha and beta subunits) that need to be produced separately.</p> Signup and view all the answers

<p>It is significantly cheaper and more effective at regulating blood glucose levels.</p> Signup and view all the answers

<p>The individual folding of the alpha and beta subunits, followed by their joining together by a disulphide bridge.</p> Signup and view all the answers

<p>To distinguish between transformed and untransformed bacteria, as only transformed bacteria with the antibiotic resistance gene will survive.</p> Signup and view all the answers

<p>A single transformation event where a bacterium has taken up a plasmid and formed a colony of identical daughter cells.</p> Signup and view all the answers

<p>To distinguish between recombinant and non-recombinant plasmids, as the reporter gene is interrupted by the gene of interest in recombinant plasmids.</p> Signup and view all the answers

<p>Because the reporter gene is continuous and fully expressed, producing a fluorescent protein that glows under UV light.</p> Signup and view all the answers

<p>Recombinant plasmids have the gene of interest inserted, interrupting the reporter gene, whereas non-recombinant plasmids do not have this insertion.</p> Signup and view all the answers

<p>To create a site for the insertion of the gene of interest, interrupting the reporter gene.</p> Signup and view all the answers