Analytical Chemistry Techniques Quiz

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What electrochemical assay measures current at fixed potential?

Anodic stripping voltammetry

Amperometry (correct)

Analysis with ISEs

Coulometry

What does reverse-phase liquid chromatography refer to?

Size used to separate solutes instead of charge

Distribution between two liquid phases

A nonpolar mobile phase and polar stationary phase

A polar mobile phase and nonpolar stationary phase (correct)

What is the false statement concerning the principles of point-of-care testing devices?

Biosensors have enabled miniaturization particularly amendable for point-of-care testing

Devices do not require quality control testing (correct)

Onboard microcomputers control instrument functions and data reduction

They use principles that are identical to laboratory-based instrumentation

What does tandem mass spectrometers refer to?

Two mass spectrometers placed in series with each other Signup and view all the answers

Which of the following refers to the movement of buffer ions and solvent relative to the fixed support?

Electroendosmosis Signup and view all the answers

What is the most sensitive detector for spectrophotometry?

Photomultiplier Signup and view all the answers

Which condition is not associated with elevated plasma uric acid concentration?

Renal disease Signup and view all the answers

In zero-order kinetics, what does it mean when the reaction is performed in zero-order kinetics?

The rate of the reaction is independent of the substrate concentration Signup and view all the answers

What happens to enzyme reaction rates with increasing temperatures until they reach denaturation?

40–60°C Signup and view all the answers

Which scenario exemplifies using enzymes as reagents in the clinical laboratory?

The hexokinase glucose method Signup and view all the answers

Why may the activity of enzymes in serum be determined rather than concentration?

The amount of enzyme is too high to measure Signup and view all the answers

When are the isoenzymes LD-4 and LD-5 elevated?

Liver disease Signup and view all the answers

Based on the flow cytometry results of DI = 2.5 and % cells in S phase = 29%, what can be concluded?

These results are consistent with a mostly diploid population Signup and view all the answers

In immunofixation protein electrophoresis of a patient with common multiple myeloma, what pattern would be expected?

Monoclonal bands of the IgG type Signup and view all the answers

What is the nucleic acid technique where RNA is converted to cDNA and then amplified called?

RT-PCR Signup and view all the answers

A serum protein electrophoresis showing decreased albumin and increased α1- and α2-globulins with normal γ-globulins is characteristic of which condition?

Cirrhosis Signup and view all the answers

Which of the following proteins is NOT part of the acute-phase reactant proteins?

Transferrin Signup and view all the answers

Which enzyme is MOST diagnostic of Duchenne-type muscular dystrophy?

Total CK level that is 50 to 100 times the ULN Signup and view all the answers

What does the saccharogenic method for amylase determinations measure?

The amount of starch present Signup and view all the answers

What causes false increases in serum enzyme measurements due to preanalytical errors?

The patient smoked three cigarettes just prior to blood collection. Signup and view all the answers

Which condition commonly presents with elevation of serum amylase and lipase?

Acute pancreatitis Signup and view all the answers

Which hormone promotes gluconeogenesis?

Hydrocortisone Signup and view all the answers

Tissue enzymes in serum may be used to detect what condition?

Tissue necrosis or damage Signup and view all the answers

What does glucose oxidase oxidize glucose to?

H2O2 Signup and view all the answers

Analytical Chemistry Techniques Quiz

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Questions and Answers

What electrochemical assay measures current at fixed potential?

What does reverse-phase liquid chromatography refer to?

What is the false statement concerning the principles of point-of-care testing devices?

What does tandem mass spectrometers refer to?

Which of the following refers to the movement of buffer ions and solvent relative to the fixed support?

What is the most sensitive detector for spectrophotometry?

Which condition is not associated with elevated plasma uric acid concentration?

In zero-order kinetics, what does it mean when the reaction is performed in zero-order kinetics?

What happens to enzyme reaction rates with increasing temperatures until they reach denaturation?

Which scenario exemplifies using enzymes as reagents in the clinical laboratory?

Why may the activity of enzymes in serum be determined rather than concentration?

When are the isoenzymes LD-4 and LD-5 elevated?

Based on the flow cytometry results of DI = 2.5 and % cells in S phase = 29%, what can be concluded?

In immunofixation protein electrophoresis of a patient with common multiple myeloma, what pattern would be expected?

What is the nucleic acid technique where RNA is converted to cDNA and then amplified called?

A serum protein electrophoresis showing decreased albumin and increased α1- and α2-globulins with normal γ-globulins is characteristic of which condition?

Which of the following proteins is NOT part of the acute-phase reactant proteins?

Which enzyme is MOST diagnostic of Duchenne-type muscular dystrophy?

What does the saccharogenic method for amylase determinations measure?

What causes false increases in serum enzyme measurements due to preanalytical errors?

Which condition commonly presents with elevation of serum amylase and lipase?

Which hormone promotes gluconeogenesis?

Tissue enzymes in serum may be used to detect what condition?

What does glucose oxidase oxidize glucose to?

Which enzyme catalyzes the formation of Glucose-6-phosphate from glucose and ATP?

What is the preferred specimen for glucose analysis?

Which hormone is the hyperglycemic factor produced by the pancreas?

What principle are polarographic methods of glucose assay based on?

Which enzyme is most specific for 𝗉d-glucose?

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