Analytical Chemistry Techniques Quiz
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Questions and Answers

What electrochemical assay measures current at fixed potential?

  • Anodic stripping voltammetry
  • Amperometry (correct)
  • Analysis with ISEs
  • Coulometry

What does reverse-phase liquid chromatography refer to?

  • Size used to separate solutes instead of charge
  • Distribution between two liquid phases
  • A nonpolar mobile phase and polar stationary phase
  • A polar mobile phase and nonpolar stationary phase (correct)

What is the false statement concerning the principles of point-of-care testing devices?

  • Biosensors have enabled miniaturization particularly amendable for point-of-care testing
  • Devices do not require quality control testing (correct)
  • Onboard microcomputers control instrument functions and data reduction
  • They use principles that are identical to laboratory-based instrumentation

What does tandem mass spectrometers refer to?

<p>Two mass spectrometers placed in series with each other (B)</p> Signup and view all the answers

Which of the following refers to the movement of buffer ions and solvent relative to the fixed support?

<p>Electroendosmosis (A)</p> Signup and view all the answers

What is the most sensitive detector for spectrophotometry?

<p>Photomultiplier (A)</p> Signup and view all the answers

Which condition is not associated with elevated plasma uric acid concentration?

<p>Renal disease (C)</p> Signup and view all the answers

In zero-order kinetics, what does it mean when the reaction is performed in zero-order kinetics?

<p>The rate of the reaction is independent of the substrate concentration (A)</p> Signup and view all the answers

What happens to enzyme reaction rates with increasing temperatures until they reach denaturation?

<p>40–60°C (C)</p> Signup and view all the answers

Which scenario exemplifies using enzymes as reagents in the clinical laboratory?

<p>The hexokinase glucose method (A)</p> Signup and view all the answers

Why may the activity of enzymes in serum be determined rather than concentration?

<p>The amount of enzyme is too high to measure (A)</p> Signup and view all the answers

When are the isoenzymes LD-4 and LD-5 elevated?

<p>Liver disease (B)</p> Signup and view all the answers

Based on the flow cytometry results of DI = 2.5 and % cells in S phase = 29%, what can be concluded?

<p>These results are consistent with a mostly diploid population (A)</p> Signup and view all the answers

In immunofixation protein electrophoresis of a patient with common multiple myeloma, what pattern would be expected?

<p>Monoclonal bands of the IgG type (B)</p> Signup and view all the answers

What is the nucleic acid technique where RNA is converted to cDNA and then amplified called?

<p>RT-PCR (A)</p> Signup and view all the answers

A serum protein electrophoresis showing decreased albumin and increased α1- and α2-globulins with normal γ-globulins is characteristic of which condition?

<p>Cirrhosis (C)</p> Signup and view all the answers

Which of the following proteins is NOT part of the acute-phase reactant proteins?

<p>Transferrin (A)</p> Signup and view all the answers

Which enzyme is MOST diagnostic of Duchenne-type muscular dystrophy?

<p>Total CK level that is 50 to 100 times the ULN (B)</p> Signup and view all the answers

What does the saccharogenic method for amylase determinations measure?

<p>The amount of starch present (B)</p> Signup and view all the answers

What causes false increases in serum enzyme measurements due to preanalytical errors?

<p>The patient smoked three cigarettes just prior to blood collection. (D)</p> Signup and view all the answers

Which condition commonly presents with elevation of serum amylase and lipase?

<p>Acute pancreatitis (C)</p> Signup and view all the answers

Which hormone promotes gluconeogenesis?

<p>Hydrocortisone (D)</p> Signup and view all the answers

Tissue enzymes in serum may be used to detect what condition?

<p>Tissue necrosis or damage (B)</p> Signup and view all the answers

What does glucose oxidase oxidize glucose to?

<p>H2O2 (A)</p> Signup and view all the answers

Which enzyme catalyzes the formation of Glucose-6-phosphate from glucose and ATP?

<p>Glucose-6-phosphate (A)</p> Signup and view all the answers

What is the preferred specimen for glucose analysis?

<p>Fluoride oxalate plasma (A)</p> Signup and view all the answers

Which hormone is the hyperglycemic factor produced by the pancreas?

<p>Glucagon (D)</p> Signup and view all the answers

What principle are polarographic methods of glucose assay based on?

<p>Rate of oxygen depletion measured (A)</p> Signup and view all the answers

Which enzyme is most specific for 𝗉d-glucose?

<p>Hexokinase (B)</p> Signup and view all the answers

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