Analytical Chemistry Techniques Quiz
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Questions and Answers

What electrochemical assay measures current at fixed potential?

  • Anodic stripping voltammetry
  • Amperometry (correct)
  • Analysis with ISEs
  • Coulometry

    • What does reverse-phase liquid chromatography refer to?

  • Size used to separate solutes instead of charge
  • Distribution between two liquid phases
  • A nonpolar mobile phase and polar stationary phase
  • A polar mobile phase and nonpolar stationary phase (correct)

    • What is the false statement concerning the principles of point-of-care testing devices?

  • Biosensors have enabled miniaturization particularly amendable for point-of-care testing
  • Devices do not require quality control testing (correct)
  • Onboard microcomputers control instrument functions and data reduction
  • They use principles that are identical to laboratory-based instrumentation

    • What does tandem mass spectrometers refer to?

    <p>Two mass spectrometers placed in series with each other</p> Signup and view all the answers

    Which of the following refers to the movement of buffer ions and solvent relative to the fixed support?

    <p>Electroendosmosis</p> Signup and view all the answers

    What is the most sensitive detector for spectrophotometry?

    <p>Photomultiplier</p> Signup and view all the answers

    Which condition is not associated with elevated plasma uric acid concentration?

    <p>Renal disease</p> Signup and view all the answers

    In zero-order kinetics, what does it mean when the reaction is performed in zero-order kinetics?

    <p>The rate of the reaction is independent of the substrate concentration</p> Signup and view all the answers

    What happens to enzyme reaction rates with increasing temperatures until they reach denaturation?

    <p>40–60°C</p> Signup and view all the answers

    Which scenario exemplifies using enzymes as reagents in the clinical laboratory?

    <p>The hexokinase glucose method</p> Signup and view all the answers

    Why may the activity of enzymes in serum be determined rather than concentration?

    <p>The amount of enzyme is too high to measure</p> Signup and view all the answers

    When are the isoenzymes LD-4 and LD-5 elevated?

    <p>Liver disease</p> Signup and view all the answers

    Based on the flow cytometry results of DI = 2.5 and % cells in S phase = 29%, what can be concluded?

    <p>These results are consistent with a mostly diploid population</p> Signup and view all the answers

    In immunofixation protein electrophoresis of a patient with common multiple myeloma, what pattern would be expected?

    <p>Monoclonal bands of the IgG type</p> Signup and view all the answers

    What is the nucleic acid technique where RNA is converted to cDNA and then amplified called?

    <p>RT-PCR</p> Signup and view all the answers

    A serum protein electrophoresis showing decreased albumin and increased α1- and α2-globulins with normal γ-globulins is characteristic of which condition?

    <p>Cirrhosis</p> Signup and view all the answers

    Which of the following proteins is NOT part of the acute-phase reactant proteins?

    <p>Transferrin</p> Signup and view all the answers

    Which enzyme is MOST diagnostic of Duchenne-type muscular dystrophy?

    <p>Total CK level that is 50 to 100 times the ULN</p> Signup and view all the answers

    What does the saccharogenic method for amylase determinations measure?

    <p>The amount of starch present</p> Signup and view all the answers

    What causes false increases in serum enzyme measurements due to preanalytical errors?

    <p>The patient smoked three cigarettes just prior to blood collection.</p> Signup and view all the answers

    Which condition commonly presents with elevation of serum amylase and lipase?

    <p>Acute pancreatitis</p> Signup and view all the answers

    Which hormone promotes gluconeogenesis?

    <p>Hydrocortisone</p> Signup and view all the answers

    Tissue enzymes in serum may be used to detect what condition?

    <p>Tissue necrosis or damage</p> Signup and view all the answers

    What does glucose oxidase oxidize glucose to?

    <p>H2O2</p> Signup and view all the answers

    Which enzyme catalyzes the formation of Glucose-6-phosphate from glucose and ATP?

    <p>Glucose-6-phosphate</p> Signup and view all the answers

    What is the preferred specimen for glucose analysis?

    <p>Fluoride oxalate plasma</p> Signup and view all the answers

    Which hormone is the hyperglycemic factor produced by the pancreas?

    <p>Glucagon</p> Signup and view all the answers

    What principle are polarographic methods of glucose assay based on?

    <p>Rate of oxygen depletion measured</p> Signup and view all the answers

    Which enzyme is most specific for 𝗉d-glucose?

    <p>Hexokinase</p> Signup and view all the answers

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