10 Questions
In which method does the detector sense light scattered by the particles?
Nephelometry
Which type of assay involves the use of antibodies?
Immunoturbidimetry
In which method does the detector directly sense the decrease in light as the number of analyte particles increases?
Turbidimetry
How does the increase in the number of analyte particles affect the amount of light reaching the detector in nephelometry?
It increases
What is the purpose of using turbidimetric and nephelometric methods in measuring proteins?
To create aggregate particles that can reflect light in the visible or ultraviolet range
How does fluorescence work in terms of light absorption and emission?
It absorbs light of higher energy and emits light of lower energy
What is the function of a chemiluminescent molecule in immunological methods for measuring hormones and tumor markers?
To generate a chemiluminescent signal proportional to the amount of analyte in the sample
Why are fluorescent molecules incorporated as reagents to help detect analytes in clinical chemistry?
To help detect analytes by generating fluorescent light proportional to their concentration
What role do antibodies with a fluorescent compound attached play in immunological methods for measuring tumor markers?
They recognize and bind to the tumor marker
What is the main purpose of using selective antibodies in immunological methods for measuring proteins and analytes?
To recognize and bind to specific proteins or analytes
Test your knowledge on turbidimetry and nephelometry, which are based on the formation of insoluble particles interfering with the passage of light through a solution. Analytes react with added reagents to produce these particles, affecting the amount of light transmitted.
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