Analytical Chemistry Techniques Quiz

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Which technique is NOT covered in the recommended text for analysis of biotechnologically-produced drugs?

Ion Exchange Chromatography (correct)

Size Exclusion Chromatography

Protein sequencing using the Edman reaction

Binding assays

What is the therapeutic action of glucagon?

Reversal of insulin-induced hypoglycaemia (correct)

Treatment of short stature due to hormone deficiency

Diabetes

Stimulation of immune system to develop B-lymphocyte memory cells

From where is human growth hormone synthesized?

Porcine thyroid

Salmon

Human pituitary gland (correct)

Bovine pancreas

What is the source of insulin in biotechnologically-produced drugs?

Bovine pancreas Signup and view all the answers

Which of the following is NOT a characteristic of proteinaceous drugs?

Treatment of short stature due to hormone deficiency Signup and view all the answers

What is the purpose of immunoglobulins in biotechnologically-produced drugs?

Offer short-term resistance to pathogens Signup and view all the answers

What is the primary factor that determines the rate of movement for anions in Capillary Electrophoresis?

EOF - ion mobility Signup and view all the answers

How does increasing the buffer strength affect the electro-osmotic flow in Capillary Electrophoresis?

Decreases EOF Signup and view all the answers

What happens to the electro-osmotic flow when anionic surfactants are introduced in Capillary Electrophoresis?

FLO increases Signup and view all the answers

Which globulin is present in higher concentrations in the cerebrospinal fluid of a patient with multiple sclerosis approaching the malignant phase?

b2-globulin Signup and view all the answers

What is the primary factor that affects the migration time in Capillary Electrophoresis?

Electro-osmotic flow Signup and view all the answers

Which NSAID was identified as peak 5 in the CE separation based on ionic radius using a buffer of 0.075 M glycine adjusted to pH 9.1 with triethanolamine?

Aceclofenac Signup and view all the answers

What is the principal of migration in capillary electrophoresis and SDSPAGE?

Migration occurs based on the charge-to-mass ratio of the proteins Signup and view all the answers

Which of the following is a potential limitation of capillary electrophoresis compared to HPLC?

Less robustness Signup and view all the answers

What is the main difference between SDSPAGE and isoelectric focusing?

SDSPAGE uses SDS, while isoelectric focusing does not Signup and view all the answers

Which of the following is a real-world pharmacopoeial analysis that uses electrophoresis?

All of the above Signup and view all the answers

What is the main advantage of capillary electrophoresis over HPLC?

Potentially many times more efficient separation power Signup and view all the answers

Which of the following is a common application of size exclusion chromatography (SEC)?

Separation of proteins based on their molecular size Signup and view all the answers

Which one of these drugs was NOT produced using CHO cells?

Remicade (infliximab) Signup and view all the answers

Which of the following is NOT an indication of Avastin (bevacizumab)?

Metastatic breast cancer Signup and view all the answers

Which statement is true about the electrophoretic mobility (me) of an ion in capillary electrophoresis?

me is proportional to the charge on the molecule and inversely proportional to the radius of the ion Signup and view all the answers

Which statement is FALSE about the separation mechanism in capillary electrophoresis?

The greater the charge on the ion, the lower its mobility Signup and view all the answers

Which of the following is NOT an application of capillary electrophoresis?

Effective for analysing drugs/metabolites in food samples Signup and view all the answers

Which statement is FALSE about the separation achieved in capillary electrophoresis?

The degree of ionisation has no bearing on the shape of the analyte in solution Signup and view all the answers

What is a downside of binding assays such as ELISA and RIA?

They can suffer from a lack of specificity Signup and view all the answers

Which technique uses a radiolabelled version of the antigen?

RIA Signup and view all the answers

Which of the following is an example of a real-world pharmacopoeial analysis using ELISA?

Identification of hepatitis B surface antigen in factor VIII Signup and view all the answers

Which of the following is an example of a real-world pharmacopoeial analysis using RIA?

Identification of hepatitis B surface antigen in urokinase Signup and view all the answers

What is the concept behind RIA?

Using a radiolabelled version of the antigen, there is a competition between labelled and unlabelled antigen, resulting in a proportional displacement of the radiolabelled version Signup and view all the answers

What is the Edman reaction?

A technique for sequencing proteins using stepwise degradation of the N-terminal amino acid Signup and view all the answers