Analytical Chemistry Techniques Quiz

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Questions and Answers

Which technique is NOT covered in the recommended text for analysis of biotechnologically-produced drugs?

  • Ion Exchange Chromatography (correct)
  • Size Exclusion Chromatography
  • Protein sequencing using the Edman reaction
  • Binding assays

What is the therapeutic action of glucagon?

  • Reversal of insulin-induced hypoglycaemia (correct)
  • Treatment of short stature due to hormone deficiency
  • Diabetes
  • Stimulation of immune system to develop B-lymphocyte memory cells

From where is human growth hormone synthesized?

  • Porcine thyroid
  • Salmon
  • Human pituitary gland (correct)
  • Bovine pancreas

What is the source of insulin in biotechnologically-produced drugs?

<p>Bovine pancreas (C)</p> Signup and view all the answers

Which of the following is NOT a characteristic of proteinaceous drugs?

<p>Treatment of short stature due to hormone deficiency (A)</p> Signup and view all the answers

What is the purpose of immunoglobulins in biotechnologically-produced drugs?

<p>Offer short-term resistance to pathogens (C)</p> Signup and view all the answers

What is the primary factor that determines the rate of movement for anions in Capillary Electrophoresis?

<p>EOF - ion mobility (B)</p> Signup and view all the answers

How does increasing the buffer strength affect the electro-osmotic flow in Capillary Electrophoresis?

<p>Decreases EOF (A)</p> Signup and view all the answers

What happens to the electro-osmotic flow when anionic surfactants are introduced in Capillary Electrophoresis?

<p>FLO increases (B)</p> Signup and view all the answers

Which globulin is present in higher concentrations in the cerebrospinal fluid of a patient with multiple sclerosis approaching the malignant phase?

<p>b2-globulin (D)</p> Signup and view all the answers

What is the primary factor that affects the migration time in Capillary Electrophoresis?

<p>Electro-osmotic flow (C)</p> Signup and view all the answers

Which NSAID was identified as peak 5 in the CE separation based on ionic radius using a buffer of 0.075 M glycine adjusted to pH 9.1 with triethanolamine?

<p>Aceclofenac (B)</p> Signup and view all the answers

What is the principal of migration in capillary electrophoresis and SDSPAGE?

<p>Migration occurs based on the charge-to-mass ratio of the proteins (A)</p> Signup and view all the answers

Which of the following is a potential limitation of capillary electrophoresis compared to HPLC?

<p>Less robustness (D)</p> Signup and view all the answers

What is the main difference between SDSPAGE and isoelectric focusing?

<p>SDSPAGE uses SDS, while isoelectric focusing does not (C)</p> Signup and view all the answers

Which of the following is a real-world pharmacopoeial analysis that uses electrophoresis?

<p>All of the above (D)</p> Signup and view all the answers

What is the main advantage of capillary electrophoresis over HPLC?

<p>Potentially many times more efficient separation power (A)</p> Signup and view all the answers

Which of the following is a common application of size exclusion chromatography (SEC)?

<p>Separation of proteins based on their molecular size (C)</p> Signup and view all the answers

Which one of these drugs was NOT produced using CHO cells?

<p>Remicade (infliximab) (D)</p> Signup and view all the answers

Which of the following is NOT an indication of Avastin (bevacizumab)?

<p>Metastatic breast cancer (A)</p> Signup and view all the answers

Which statement is true about the electrophoretic mobility (me) of an ion in capillary electrophoresis?

<p>me is proportional to the charge on the molecule and inversely proportional to the radius of the ion (C)</p> Signup and view all the answers

Which statement is FALSE about the separation mechanism in capillary electrophoresis?

<p>The greater the charge on the ion, the lower its mobility (D)</p> Signup and view all the answers

Which of the following is NOT an application of capillary electrophoresis?

<p>Effective for analysing drugs/metabolites in food samples (B)</p> Signup and view all the answers

Which statement is FALSE about the separation achieved in capillary electrophoresis?

<p>The degree of ionisation has no bearing on the shape of the analyte in solution (A)</p> Signup and view all the answers

What is a downside of binding assays such as ELISA and RIA?

<p>They can suffer from a lack of specificity (A)</p> Signup and view all the answers

Which technique uses a radiolabelled version of the antigen?

<p>RIA (A)</p> Signup and view all the answers

Which of the following is an example of a real-world pharmacopoeial analysis using ELISA?

<p>Identification of hepatitis B surface antigen in factor VIII (C)</p> Signup and view all the answers

Which of the following is an example of a real-world pharmacopoeial analysis using RIA?

<p>Identification of hepatitis B surface antigen in urokinase (B)</p> Signup and view all the answers

What is the concept behind RIA?

<p>Using a radiolabelled version of the antigen, there is a competition between labelled and unlabelled antigen, resulting in a proportional displacement of the radiolabelled version (C)</p> Signup and view all the answers

What is the Edman reaction?

<p>A technique for sequencing proteins using stepwise degradation of the N-terminal amino acid (A)</p> Signup and view all the answers

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