Alcohol-based Hand Rubbing Efficacy

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Questions and Answers

What is one reason alcohol-based hand rubs have replaced conventional hand-washing agents?

They require more time to use.

They produce fewer skin reactions. (correct)

They are not effective against bacteria.

They are less effective than soap and water.

Which of the following is an ingredient commonly found in an alcohol-based hand sanitizer?

4% chlorhexidine gluconate

10% povidone iodine

0.15% triclosan

65% ethanol (correct)

What is the recommended duration for rubbing the thumb into the hand-cleansing agent?

20 seconds

5 seconds

15 seconds

10 seconds (correct)

Which of these hand-cleaning agents is NOT mentioned as a standard option?

Regular soap without antibacterial properties Signup and view all the answers

What should you do after using the hand-cleansing agent?

Wash both hands with soap and water. Signup and view all the answers

What is the hottest part of the flame used for sterilizing inoculating instruments?

Inner cone Signup and view all the answers

What is the primary purpose of broths in microbial culture?

To grow large numbers of cells Signup and view all the answers

How should the inoculating loop be held for convenient tube cap handling?

Like a pencil Signup and view all the answers

Which media type is typically used for maintaining stock cultures?

Agar slants Signup and view all the answers

What is an example of an exception in transferring culture media?

A streak plate Signup and view all the answers

What is the first step in the aseptic transfer procedure?

Label the sterile agar slant Signup and view all the answers

How should the tube be held while flaming its lip?

At an angle with the agar surface facing upward Signup and view all the answers

What action should be taken after flaming the loop?

Touch the agar with the tip of the loop Signup and view all the answers

What is the purpose of moving the loop in a zigzag pattern on the agar surface?

To spread the inoculum evenly without cutting the agar Signup and view all the answers

At what point should the inoculated tube be returned to the test tube rack?

Once the transfer is complete Signup and view all the answers

Why is it important to let the loop get uniformly orange-hot?

It ensures the loop is clean for the transfer Signup and view all the answers

What should be done immediately after removing the culture tube's cap?

Flame the tube's lip Signup and view all the answers

Which step must be done carefully to avoid contaminating the culture?

Removing the cap with the little finger of your loop hand Signup and view all the answers

What is the final step after performing the inoculation?

Seal the inoculated culture with the cap Signup and view all the answers

What is the recommended temperature for incubating the inoculated culture?

At the assigned temperature specific to the organism Signup and view all the answers

Study Notes

Hand-Washing Agents

Alcohol-based hand rubs often replace soap due to higher effectiveness and convenience.
Types of hand sanitizers include:
- Betadine® scrub (10% povidone iodine)
- Alcohol-based sanitizer (65% ethyl alcohol)
- Benzalkonium chloride sanitizer (0.13%)
- Antibacterial soap (0.15% triclosan)
- Hibiclens® (4% chlorhexidine gluconate)

Aseptic Technique in Microbiology

Importance of using sterilized loops:
- Flamed to remove contaminants
- Used in transferring cultures between media
Growth mediums include:
- Broths for large quantities and identification
- Agar slants for refrigerator-storable cultures
Procedures involve careful handling of tubes, flaming lips, and touching loops to agar without cutting it.

Culture Transfer Methodology

For inoculating from a nutrient agar slant to a sterile agar slant:
- Label slant with essential information (name, date, medium, organism).
- Maintain sterility by flaming loop and tube mouths properly.
- Use zigzag motion while moving the loop over agar surface.
Growth can be assessed in broths qualitatively using turbidity scores, indicating levels from not turbid to very turbid.

Growth Observation and Analysis

Evaluate the source of growth and turbidity differences in cultures.
Personal experiences and observations enhance understanding and troubleshooting of microbiological techniques.

Streak Plate Isolation

Distinction between mixed cultures (multiple species) and pure cultures (single species).
Purpose is to isolate individual cells to form distinct colonies on agar surfaces, facilitating the study of specific microorganisms.

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Description

This quiz explores the advantages of alcohol-based hand rubs over conventional hand-washing techniques. It discusses their effectiveness, time efficiency, and impact on skin reactions. Gain insights into proper hand hygiene practices.