18 Questions
What method is best used for detecting, confirming, and characterizing monoclonal gammopathies?
Immunofixation electrophoresis
Which technique involves the application of antiserum directly to the surface of a gel after electrophoresis is performed?
Immunofixation electrophoresis
What method enhances the semiquantitation of antigens?
Immunoelectrophoresis
Which process involves forming large, visible aggregates if a specific antibody is present?
Agglutination
What is a disadvantage of agglutination reactions?
Requires complicated equipment
Which test is considered the best adaptation of Immunofixation electrophoresis?
Western Blot test
Which technique is an excellent screening test to differentiate serum proteins and detect abnormalities in myelomas and lymphomas?
Immunoelectrophoresis
Which technique involves a two-step double-diffusion process?
Immunoelectrophoresis
What is the primary advantage of agglutination reactions?
Can be easily performed with no complicated equipment
In Immunofixation electrophoresis (IFE), what is the main difference compared to Immunoelectrophoresis (IEP)?
Antiserum is not applied directly to the gel surface
What characterizes the relationship between antigen and antibody in agglutination reactions?
They specifically interact to form large, visible aggregates
What is a key distinguishing feature of the Western Blot test when compared to other techniques?
It is an adaptation of Immunofixation electrophoresis
What is a disadvantage of Immunoelectrophoresis (IEP) when compared to other techniques?
It is less sensitive in detecting monoclonal gammopathies
Which process involves applying antiserum directly to the surface of a gel post-electrophoresis?
Immunofixation electrophoresis (IFE)
What effect does the presence of a specific antibody have in agglutination reactions?
Leads to the formation of large, visible aggregates
What makes Western Blot test well-suited for detecting monoclonal gammopathies?
It is considered the best adaptation for this purpose.
In what way does agglutination differ from other immunological tests?
It can be easily performed without complicated equipment.
What is a notable limitation of Immunoelectrophoresis (IEP) compared to other methods?
It lacks sensitivity in detecting certain disorders.
Test your knowledge on the types of agglutination reactions commonly used in laboratory settings, including direct agglutination, passive agglutination, reverse passive agglutination, and more. Learn about the process of sensitization, lattice formation, and various applications of agglutination reactions.
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