5 Questions
What determines the molecular sieving properties of an agarose gel?
The concentration of agarose
Which technique is used to separate DNA fragments smaller than 100 bp?
Polyacrylamide gel electrophoresis
What is the main function of the microscopic pores in an agarose gel during electrophoresis?
Act as a molecular sieve
What type of biomolecules can be separated using agarose gel electrophoresis?
RNA, DNA, and proteins
Where is agarose isolated from to make the separation medium for electrophoresis?
Seaweed genera Gelidium and Gracilaria
Study Notes
Agarose Gel Electrophoresis
- The molecular sieving properties of an agarose gel are determined by the pore size of the agarose matrix, which is influenced by the concentration of agarose in the gel.
Separation of DNA Fragments
- DNA fragments smaller than 100 bp are separated using polyacrylamide gel electrophoresis (PAGE).
Function of Microscopic Pores
- The microscopic pores in an agarose gel during electrophoresis act as a sieve, allowing smaller molecules to pass through while larger molecules are retained.
Biomolecules Separation
- Agarose gel electrophoresis is used to separate various biomolecules, including DNA, RNA, and proteins.
Source of Agarose
- Agarose is isolated from red algae, specifically from the cell walls of red algae, to make the separation medium for electrophoresis.
Test your knowledge of agarose gel electrophoresis, a common technique used to separate DNA fragments based on charge, size, and shape. Explore its applications and resolving power in DNA analysis.
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