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Questions and Answers
What determines the molecular sieving properties of an agarose gel?
What determines the molecular sieving properties of an agarose gel?
- The size of the pre-cast wells in the gel
- The application of current
- The presence of seaweed genera Gelidium and Gracilaria
- The concentration of agarose (correct)
Which technique is used to separate DNA fragments smaller than 100 bp?
Which technique is used to separate DNA fragments smaller than 100 bp?
- Restriction enzyme analysis
- Polyacrylamide gel electrophoresis (correct)
- Pulse-field gel electrophoresis
- Agarose gel electrophoresis
What is the main function of the microscopic pores in an agarose gel during electrophoresis?
What is the main function of the microscopic pores in an agarose gel during electrophoresis?
- Generate current
- Facilitate DNA loading
- Act as a molecular sieve (correct)
- Provide structural support
What type of biomolecules can be separated using agarose gel electrophoresis?
What type of biomolecules can be separated using agarose gel electrophoresis?
Where is agarose isolated from to make the separation medium for electrophoresis?
Where is agarose isolated from to make the separation medium for electrophoresis?
Flashcards
Agarose Gel Sieving
Agarose Gel Sieving
Agarose gel molecular sieving is determined by its concentration.
Separate Small DNA Fragments
Separate Small DNA Fragments
Polyacrylamide gel electrophoresis is used for separating DNA fragments smaller than 100 bp.
Pores in Agarose Gel
Pores in Agarose Gel
Microscopic pores act as a molecular sieve, separating molecules by size.
Agarose Uses
Agarose Uses
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Source of Agarose
Source of Agarose
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Study Notes
Agarose Gel Electrophoresis
- The molecular sieving properties of an agarose gel are determined by the pore size of the agarose matrix, which is influenced by the concentration of agarose in the gel.
Separation of DNA Fragments
- DNA fragments smaller than 100 bp are separated using polyacrylamide gel electrophoresis (PAGE).
Function of Microscopic Pores
- The microscopic pores in an agarose gel during electrophoresis act as a sieve, allowing smaller molecules to pass through while larger molecules are retained.
Biomolecules Separation
- Agarose gel electrophoresis is used to separate various biomolecules, including DNA, RNA, and proteins.
Source of Agarose
- Agarose is isolated from red algae, specifically from the cell walls of red algae, to make the separation medium for electrophoresis.
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