5 Questions
What is the principle behind affinity chromatography?
Specific binding interactions between the analyte of interest and a stationary phase with an immobilized ligand
What type of interactions are frequently exploited for isolation of various biomolecules in affinity chromatography?
Antigen and antibody, enzyme and substrate, receptor and ligand, or protein and nucleic acid binding interactions
What is the advantage of affinity chromatography?
High selectivity and resolution of separation compared to other chromatographic methods
In a typical affinity chromatography experiment, what is the ligand attached to?
A solid, insoluble matrix chemically modified to introduce a binding partner or ligand
What does affinity chromatography rely on for the separation of biomolecules?
Specific binding interactions between the analyte of interest and an immobilized ligand
Test your knowledge of affinity chromatography with this quiz! Explore the principles and applications of this method, including specific binding interactions such as antigen-antibody, enzyme-substrate, receptor-ligand, and protein-nucleic acid interactions.
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