Affinity Chromatography Quiz

Use of high pressure to separate biomolecules from a mixture

Utilization of magnetic fields to isolate biomolecules

Dependence on differences in molecular weight for separation

Specific binding interactions between the analyte of interest and a stationary phase with an immobilized ligand (correct)

Covalent bonding between molecules

Hydrophobic interactions between molecules

Ionic interactions between molecules

Antigen and antibody, enzyme and substrate, receptor and ligand, or protein and nucleic acid binding interactions (correct)

Ability to separate biomolecules based on color differences

Fast separation of biomolecules

Low cost compared to other separation techniques

High selectivity and resolution of separation compared to other chromatographic methods (correct)

<p>A solid, insoluble matrix chemically modified to introduce a binding partner or ligand</p> Signup and view all the answers

<p>Specific binding interactions between the analyte of interest and an immobilized ligand</p> Signup and view all the answers