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What is the principle behind affinity chromatography?
What is the principle behind affinity chromatography?
- Use of high pressure to separate biomolecules from a mixture
- Utilization of magnetic fields to isolate biomolecules
- Dependence on differences in molecular weight for separation
- Specific binding interactions between the analyte of interest and a stationary phase with an immobilized ligand (correct)
What type of interactions are frequently exploited for isolation of various biomolecules in affinity chromatography?
What type of interactions are frequently exploited for isolation of various biomolecules in affinity chromatography?
- Covalent bonding between molecules
- Hydrophobic interactions between molecules
- Ionic interactions between molecules
- Antigen and antibody, enzyme and substrate, receptor and ligand, or protein and nucleic acid binding interactions (correct)
What is the advantage of affinity chromatography?
What is the advantage of affinity chromatography?
- Ability to separate biomolecules based on color differences
- Fast separation of biomolecules
- Low cost compared to other separation techniques
- High selectivity and resolution of separation compared to other chromatographic methods (correct)
In a typical affinity chromatography experiment, what is the ligand attached to?
In a typical affinity chromatography experiment, what is the ligand attached to?
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What does affinity chromatography rely on for the separation of biomolecules?
What does affinity chromatography rely on for the separation of biomolecules?
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