18 Questions
In ion-exchange chromatography, which molecules bind tightly to the resin?
Molecules possessing the opposite charge as the resin
What is the key factor that determines the elution order in liquid column chromatography?
Binding affinity of the analyte molecule to the stationary phase
Which chromatography technique primarily depends on the volatilization of samples?
Gas chromatography
What happens to unbound analytes in affinity chromatography?
They pass through in the mobile phase and elute out of the column
What type of support is used in size exclusion chromatography?
Solid support
Which application does NOT fall under the category of liquid chromatography?
Gas testing
Which chromatographic technique uses solid silica or alumina as the stationary phase and liquid as the mobile phase?
Thin layer chromatography (TLC)
In which chromatographic technique is size exclusion utilized as the basis of separation?
Size exclusion chromatography
Which of the following chromatographic techniques involves no interaction, physical or chemical, between the analyte and the stationary phase?
Size exclusion chromatography
Which chromatographic technique uses solid cellulose as the stationary phase and liquid as the mobile phase?
Paper chromatography
In which chromatographic technique is a glass column packed with a slurry of silica to separate components based on polarity?
Liquid column chromatography
Which of the following chromatographic techniques involves spotting compounds directly on a cellulose paper to separate based on polarity?
Paper chromatography
In paper chromatography, what acts as the stationary phase?
Cellulose
What is the purpose of the solvent in paper chromatography?
To separate the components of the analyte
How do components that adhere more strongly to the stationary phase travel in paper chromatography?
Less quickly
What is the purpose of the silica bed in liquid column chromatography?
To act as the stationary phase
What can analytical chromatography be used for?
Purifying compounds in milligram to gram scale
What phenomenon allows the solvent to rise up the paper strip in paper chromatography?
Capillary action
Test your knowledge on ion-exchange chromatography and affinity chromatography techniques. Learn how molecules interact with solid resins and immobilized molecules in liquid solutions.
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