Chromatography Techniques Quiz

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Questions and Answers

In ion-exchange chromatography, which molecules bind tightly to the resin?

  • Molecules with a positive charge
  • Molecules with the same charge as the resin
  • Molecules with a neutral charge
  • Molecules possessing the opposite charge as the resin (correct)

What is the key factor that determines the elution order in liquid column chromatography?

  • Molecular weight of the analyte
  • Binding affinity of the analyte molecule to the stationary phase (correct)
  • Charge of the analyte
  • Boiling point of the analyte

Which chromatography technique primarily depends on the volatilization of samples?

  • Paper chromatography
  • Gas chromatography (correct)
  • Size exclusion chromatography
  • Thin layer chromatography (TLC)

What happens to unbound analytes in affinity chromatography?

<p>They pass through in the mobile phase and elute out of the column (B)</p> Signup and view all the answers

What type of support is used in size exclusion chromatography?

<p>Solid support (B)</p> Signup and view all the answers

Which application does NOT fall under the category of liquid chromatography?

<p>Gas testing (A)</p> Signup and view all the answers

Which chromatographic technique uses solid silica or alumina as the stationary phase and liquid as the mobile phase?

<p>Thin layer chromatography (TLC) (B)</p> Signup and view all the answers

In which chromatographic technique is size exclusion utilized as the basis of separation?

<p>Size exclusion chromatography (B)</p> Signup and view all the answers

Which of the following chromatographic techniques involves no interaction, physical or chemical, between the analyte and the stationary phase?

<p>Size exclusion chromatography (A)</p> Signup and view all the answers

Which chromatographic technique uses solid cellulose as the stationary phase and liquid as the mobile phase?

<p>Paper chromatography (B)</p> Signup and view all the answers

In which chromatographic technique is a glass column packed with a slurry of silica to separate components based on polarity?

<p>Liquid column chromatography (A)</p> Signup and view all the answers

Which of the following chromatographic techniques involves spotting compounds directly on a cellulose paper to separate based on polarity?

<p>Paper chromatography (C)</p> Signup and view all the answers

In paper chromatography, what acts as the stationary phase?

<p>Cellulose (D)</p> Signup and view all the answers

What is the purpose of the solvent in paper chromatography?

<p>To separate the components of the analyte (C)</p> Signup and view all the answers

How do components that adhere more strongly to the stationary phase travel in paper chromatography?

<p>Less quickly (B)</p> Signup and view all the answers

What is the purpose of the silica bed in liquid column chromatography?

<p>To act as the stationary phase (D)</p> Signup and view all the answers

What can analytical chromatography be used for?

<p>Purifying compounds in milligram to gram scale (C)</p> Signup and view all the answers

What phenomenon allows the solvent to rise up the paper strip in paper chromatography?

<p>Capillary action (D)</p> Signup and view all the answers

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