Podcast
Questions and Answers
What is the role of the sigma factor in prokaryotic transcription initiation?
What is the role of the sigma factor in prokaryotic transcription initiation?
- It facilitates translation initiation
- It aids in mRNA processing
- It helps RNA polymerase recognize the promoter region (correct)
- It assists in DNA replication
During mRNA synthesis, where does the energy come from to create bonds between nucleotides?
During mRNA synthesis, where does the energy come from to create bonds between nucleotides?
- GTP hydrolysis
- Hydrolysis of pyrophosphate from incoming nucleotides (correct)
- ATP hydrolysis
- Energy from tRNA molecules
What is the importance of the 2’, 3’, and 5’ carbons of RNA (and/or DNA)?
What is the importance of the 2’, 3’, and 5’ carbons of RNA (and/or DNA)?
- They determine the nucleotide sequence
- They are responsible for mRNA stability
- They are involved in forming phosphodiester bonds (correct)
- They regulate transcription initiation
What do we mean when we say the genetic code is “universal”?
What do we mean when we say the genetic code is “universal”?
What is the role of tRNA? How are tRNAs “charged”?
What is the role of tRNA? How are tRNAs “charged”?
What enzyme is used in PCR? What enzymatic activity does it have?
What enzyme is used in PCR? What enzymatic activity does it have?
If the template strand is used as template to produce RNA, why do we call the nontemplate strand the “coding strand”?
If the template strand is used as template to produce RNA, why do we call the nontemplate strand the “coding strand”?
What components of the replication fork are not present in PCR, and why are they not needed?
What components of the replication fork are not present in PCR, and why are they not needed?
What is the genetic code “degenerate”?
What is the genetic code “degenerate”?
Where in the eukaryotic cell do transcription, mRNA processing, and translation occur? What makes this different than prokaryotes?
Where in the eukaryotic cell do transcription, mRNA processing, and translation occur? What makes this different than prokaryotes?
