Advanced Molecular Biology Quiz
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Questions and Answers

What is the role of the sigma factor in prokaryotic transcription initiation?

  • It helps RNA polymerase recognize the promoter sequence (correct)
  • It aids in the binding of ribosomes to mRNA
  • It facilitates the splicing of pre-mRNA
  • It acts as a proofreading enzyme during transcription
  • During mRNA synthesis, where does the energy come from to create bonds between nucleotides?

  • The energy is provided by the binding of RNA polymerase to the template DNA strand
  • Nucleotide triphosphates provide the energy for bond formation (correct)
  • Phosphodiester bonds are formed using energy from the hydrolysis of ATP
  • The energy is derived from the hydrolysis of DNA
  • What enzyme is used in PCR and what enzymatic activity does it have?

  • DNA helicase, which unwinds the double helix during DNA replication
  • DNA polymerase I, which removes RNA primers and fills the gaps with DNA nucleotides
  • Taq polymerase, which synthesizes new DNA strands using existing ones as templates (correct)
  • DNA ligase, which joins Okazaki fragments during lagging strand synthesis
  • RNA polymerase, responsible for transcribing DNA into RNA
  • What do we mean when we say the genetic code is 'degenerate'?

    <p>Multiple codons can code for the same amino acid</p> Signup and view all the answers

    What is the importance of the 2’, 3’, and 5’ carbons of RNA (and/or DNA)?

    <p>They are involved in the formation of phosphodiester bonds</p> Signup and view all the answers

    What is the role of tRNA and how are tRNAs 'charged'?

    <p>tRNA is responsible for translating the genetic code into a specific sequence of amino acids; charging involves the attachment of an amino acid</p> Signup and view all the answers

    What is the difference between DNA, gene, codon, exon, and chromosome?

    <p>A gene is a segment of DNA that contains the instructions for making a specific protein; a codon is a sequence of nucleotides that specifies a particular amino acid; an exon is a non-coding region of DNA; a chromosome is a single-stranded DNA molecule</p> Signup and view all the answers

    Where in the eukaryotic cell do transcription, mRNA processing, and translation occur? What makes this different than prokaryotes?

    <p>Transcription occurs in the nucleus, while mRNA processing and translation occur in the cytoplasm; in prokaryotes, all these processes occur in the cytoplasm</p> Signup and view all the answers

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