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Advanced Molecular Techniques: Blotting Techniques

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Advanced Molecular Techniques: Blotting Techniques

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Questions and Answers

What is the purpose of DNA purification in Southern blotting?

To cut DNA into different sized pieces

To add a labeled probe for hybridization

To transfer DNA to a solid support

To isolate DNA from different tissues (correct)

Why is denaturation of DNA necessary in Southern blotting?

To transfer DNA to a solid support

To add a labeled probe for hybridization

To convert double-stranded DNA into single-stranded DNA (correct)

To cut DNA into different sized pieces

What is the function of gel electrophoresis in the Southern blotting process?

Add a labeled probe for hybridization

Sort the DNA pieces by size (correct)

Hydrolyze the bonds within the DNA strand

Promote cell lysis

Why is it important to add a labeled probe in Southern blotting?

To visualize and detect specific DNA sequences Signup and view all the answers

What is the significance of autoradiography in Southern blotting?

To expose X-ray film where probe is bound to the filter Signup and view all the answers

What role does washing play in the Southern blotting procedure?

To wash away excess probe and reduce background Signup and view all the answers

What is the purpose of Southern blotting?

To detect DNA Signup and view all the answers

Who is credited with inventing the Southern blotting technique?

Edwin Mellor Southern Signup and view all the answers

What is the main purpose of hybridization in Southern blotting?

Form double-stranded DNA between probe and target Signup and view all the answers

In Southern blotting, what material are DNA fragments transferred to?

Nitrocellulose filter Signup and view all the answers

What is the first step in performing Southern blotting?

Extract and purify DNA from cells Signup and view all the answers